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The Chlamydomonas Sourcebook

Volume 2: Organellar and Metabolic Processes

  • 3rd Edition - February 15, 2023
  • Latest edition
  • Editors: Arthur Grossman, Francis-André Wollman
  • Language: English

Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydo… Read more

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Description

Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new knowledge and resources for the Chlamydomonas community. It details the tremendous progress recently made with respect to imaging the ultrastructure of cells, dissecting acclimation and biosynthetic responses, and elucidating molecular processes underlying the biology of organelles. In particular, this volume includes exciting new developments in the use of imaging technologies for examining supramolecular organization of the chloroplast, defining mechanisms of branched electron transfer pathways in photosynthesis, dissecting the organization of pyrenoids and CO2 concentration mechanisms, presenting the intricacies associated with acclimation to environmental conditions and providing new insights into dark metabolism and the network of fermentative metabolism.

This book thus presents the latest advances in both the research and uses of new experimental approaches and technologies, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, aspects of human disease, acclimation to environmental change, and the biogenesis of cellular complexes.

Key features

  • Describes molecular techniques, analysis of the recently sequenced genome, reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism
  • Provides methods for Chlamydomonas research and best practices for their applications; this includes methods for cell culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media, classical genetic manipulation, and new approaches for gene transfer and editing technologies
  • Assists researchers with common laboratory problems such as contamination

Readership

Researchers in plant science who study photosynthesis; crop scientists; fertility researchers; mammalian vision researchers; biochemistry researchers; plant physiologists; plant and molecular biologists and even human disease; University and Medical libraries. The book will also be of extreme value to both postdoctoral and graduate students

Table of contents

1. The Comparative, Biochemistry, Genetics and Evolution of Starch Metabolism in Chlamydomonas reinhardtii

2. Chlamydomonas Glycerolipid Metabolism

3. Nitrogen Metabolism in Chlamydomonas

4. Phosphorus and Sulfur Uptake, Assimilation, and Deprivation Responses

5. Trace metal nutrition and response to deficiency

6. Sensory photoreceptors in Chlamydomonas

7. RubisCo and Carbon assimilation

8. Photoproduction of reducing power and the Calvin-Benson Cycle

9. Metabolic Networks during Dark Anoxia

10. Hydrogenases and hydrogen production

11. The mitochondrion: from genome to proteome

12. The chloroplast in a changing environment: from genome to proteome

13. Control of organellar gene expression by nucleus-encoded proteins

14. Translation and Protein Synthesis in the chloroplast

15. Photosynthesis: Light Harvesting

16. Photosystem I and II

17. Chloroplast ATP synthase and the cytochrome b6f complex

18. The multiple routes of photosynthetic electron transfer in Chlamydomonas reinhardtii

19. Assembly of Photosynthetic proteins

20. Molecular chaperones and proteases

21. Tetrapyrrole biosynthesis and signaling (chlorophyll heme and bilins)

22. Carotenoids in Chlamydomonas

23. Supramolecular organization of chloroplast membranes

24. State transitions

25. Photoprotection

Product details

  • Edition: 3
  • Latest edition
  • Published: February 22, 2023
  • Language: English

About the editors

AG

Arthur Grossman

Arthur R. Grossman has been a staff scientist at the Carnegie Institution for Science since 1982 and is a courtesy professor at Stanford University. He received both the Gilbert Morgan Smith Medal (2009) from the National Academy of Sciences and the Darbaker Prize (2002) from the Botanical Society of America for his work on microalga. In an NSF-supported project and collaborations with the Joint Genome Institute of the Department of Energy (DOE), he spearheaded the initial Chlamydomonas genome project that led to the complete Chlamydomonas genome sequence, its initial annotation, and the use of the information to promote genome-wide transcriptome analyses; Chlamydomonas remains a powerful molecular-genomic model system and a flagship alga of the DOE. Grossman’s focus is on how photosynthetic organisms perceive and respond to their environment, with an emphasis on light and nutrient conditions.
Affiliations and expertise
Staff scientist, Carnegie Institution for Science Courtesy professor, Stanford University.

FW

Francis-André Wollman

Francis-André Wollman is an emeritus research director at the Centre National de la Recherche Scientifique (CNRS), a member of the French Academy of Sciences, a member of EMBO, and has received the Silver Medal from the CNRS. In the mid-1970s, he joined the photosynthesis laboratory of Pierre Joliot at the Institut de Biologie Physico-Chimique (IBPC) in Paris and in the late 1990s, he became the director of this laboratory before being appointed director of IBPC in 2007. His research has focused on the biogenesis, regulation, and evolution of oxygenic photosynthesis. Throughout his career, he has used the genetics of Chlamydomonas reinhardtii for biophysical, biochemical, and structural studies to provide a dynamic view of photosynthesis as being highly responsive to an ever-changing environment through its bioenergetic integration and metabolic flexibility.
Affiliations and expertise
Emeritus research director, Centre National de la Recherche Scientifique (CNRS).

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