The Chlamydomonas Sourcebook
Volume 3: Cell Motility and Behavior
- 3rd Edition - February 15, 2023
- Imprint: Academic Press
- Editor: Susan Dutcher
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 8 2 2 5 0 8 - 0
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 2 5 0 9 - 7
The Chlamydomonas Sourcebook, 3rd Edition Cell Motility and Behavior (Volume 3) The gold-standard reference introducing this multidisciplinary science, fully revised and u… Read more
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Cell Motility and Behavior (Volume 3)
The gold-standard reference introducing this multidisciplinary science, fully revised and updated with the latest discoveries
Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the third volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Cell Motility and Behavior has been fully revised and updated to include the wealth of new resources for the Chlamydomonas community. Reflecting the significant advancement in the understanding of the role of basal bodies and cilia play in human diseases, this volume employs quantitative proteomics and mass spectroscopy as well as cryo EM tomography and single particle cryo EM. Other topics such as current insights on mitosis and cytokinesis, ciliary assembly and motility, intraflagellar transport, and more help build an understanding of human diseases of the cilium.
Cell Motility and Behavior presents the latest in research and best practices, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists.
- Provides an essential reference to a model species for the study of mechanisms of motility in free living cells
- Includes methods for Chlamydomonas motility research
- Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process
Researchers in plant science who study photosynthesis; crop scientists; fertility researchers; mammalian vision researchers; biochemistry researchers; plant physiologists; plant and molecular biologists and even human disease; University and Medical libraries. The book will also be of extreme value to both postdoctoral and graduate students
- Cover Image
- Title page
- Table of Contents
- Copyright
- List of contributors
- Preface to the Third Edition of The Chlamydomonas Sourcebook
- IntroductiontoVolume3
- Chapter 1. Landmark contributions of Chlamydomonas to understanding cilia
- Abstract
- 1.1 Landmark contributions of Chlamydomonas
- 1.2 Dikaryons
- 1.3 Tip assembly and ciliary dynamics
- 1.4 Chlamydomonas mutants: screens, genes, and axonemes
- 1.5 Intraflagellar transport (IFT): combining genetics, biochemistry, and live imaging
- 1.6 The basal bodies
- 1.7 The transition zone
- 1.8 Advances in electron microscopy to understand ciliary structures
- 1.9 Signaling
- 1.10 Key questions and directions for the future
- References
- Chapter 2. Basal bodies
- Abstract
- 2.1 Introduction
- 2.2 Basal bodies architecture
- 2.3 Assembly of basal bodies
- 2.4 Function of basal bodies
- 2.5 Basal bodies components: identification, localization, and cellular phenotypes
- 2.6 Conclusion
- References
- Chapter 3. The ciliary transition zone
- Abstract
- 3.1 Introduction
- 3.2 Defining the transition zone
- 3.3 Structures of the transition zone
- 3.4 The transition zone and the cell cycle
- 3.5 Composition of the transition zone
- 3.6 Function of the transition zone
- 3.7 Conclusion
- References
- Chapter 4. Axonemal dyneins: genetics, structure, and motor activity
- Abstract
- 4.1 Introduction
- 4.2 Arrangement of dyneins in the axoneme
- 4.3 Dynein genetics
- 4.4 Dynein heavy chains
- 4.5 Composition and physical properties of axonemal dyneins
- 4.6 Axonemal dyneins as motor proteins
- 4.7 Conclusions and prospects
- References
- Chapter 5. Axonemal dynein preassembly
- Abstract
- 5.1 Introduction
- 5.2 Cytoplasmic preassembly of axonemal dynein complexes
- 5.3 Postassembly maturation of axonemal dyneins
- 5.4 IFT-associated transport of preassembled/matured dyneins
- 5.5 Final docking of IFT-transported dyneins onto doublet microtubules
- 5.6 Dynein preassembly in other organisms
- 5.7 Conclusions and Prospects
- Acknowledgements
- References
- Chapter 6. Ciliary radial spokes
- Abstract
- 6.1 Introduction
- 6.2 Genetic dissection of radial spokes
- 6.3 Biochemical and Structural characterization of radial spokes
- 6.4 Assembly of radial spokes
- 6.5 Radial spoke function
- 6.6 Future directions
- References
- Chapter 7. Chlamydomonas ciliary central apparatus
- Abstract
- 7.1 Introduction
- 7.2 Structure of the central apparatus
- 7.3 The central apparatus proteome
- 7.4 Putting the pieces together—location of proteins within the central apparatus
- 7.5 Central apparatus regulation of ciliary motility
- 7.6 Central apparatus assembly
- 7.7 Contributions of Chlamydomonas central apparatus studies to understanding human disease
- 7.8 Future directions
- References
- Chapter 8. I1/f dynein and the nexin–dynein regulatory complex form two hubs to control dynein activity and ciliary beating
- Abstract
- 8.1 Introduction
- 8.2 The central apparatus and radial spokes as regulators of dynein activity
- 8.3 Mutations that alter outer dynein arms activity
- 8.4 Mutations that alter I1//f dynein activity
- 8.5 DRC mutations that alter the assembly of single-headed inner dynein arms
- 8.6 Identification and localization of dynein regulatory complex subunits within the nexin link
- 8.7 Characterization of other subcomplexes in the axoneme that interact with the N-DRC
- 8.8 Function of the nexin-dynein regulatory complex link and future directions
- References
- Chapter 9. Ciliary asymmetries and their role in ciliary motility
- Abstract
- 9.1 Introduction
- 9.2 Cis versus trans asymmetries
- 9.3 Proximal versus distal asymmetry
- 9.4 Proximal and radial asymmetries
- 9.5 Radial asymmetries
- 9.6 Conclusions and future directions
- References
- Chapter 10. The Chlamydomonas ciliary membrane and its dynamic properties
- Abstract
- 10.1 The cilium as a membrane-bounded organelle
- 10.2 Structure of the ciliary membrane
- 10.3 Components of the ciliary membrane
- 10.4 Assembly, disassembly, and turnover of the ciliary membrane
- 10.5 Functions of the ciliary membrane
- 10.6 Summary
- References
- Chapter 11. Physics and mechanics of ciliary beating
- Abstract
- 11.1 Motivation and background
- 11.2 Fundamental principles and concepts
- 11.3 Mechanics of waveform generation
- 11.4 Synchronization and collective dynamics of cilia
- 11.5 Environmental feedback and control of whole-cell movement
- 11.6 Conclusions and outlook
- References
- Chapter 12. Intraflagellar transport
- Abstract
- 12.1 Introduction to intraflagellar transport
- 12.2 The intraflagellar transport machinery
- 12.3 The intraflagellar transport cycle
- 12.4 Intraflagellar transport-dependent protein transport
- 12.5 Other roles of intraflagellar transport
- 12.6 Summary
- References
- Chapter 13. Genetic control of cilia length in Chlamydomonas
- Abstract
- 13.1 Introduction
- 13.2 Is ciliary length actively controlled?
- 13.3 Genetic analysis of ciliary length control
- 13.4 Is length regulated by controlling assembly, disassembly, or both?
- 13.5 Possible biochemical evidence for length sensing: aurora kinase
- 13.6 Conclusion
- References
- Chapter 14. Ciliary disassembly
- Abstract
- 14.1 Introduction
- 14.2 Ciliary disassembly under physiological conditions
- 14.3 Ciliary disassembly under non-physiological conditions
- 14.4 The fate of ciliary proteins during ciliary disassembly
- 14.5 Post-TraNslational modification of ciliary proteins during disassembly
- 14.6 Depolymerization of axonemal microtubules
- 14.7 Protein transport and intraflagellar transport during ciliary disassembly
- 14.8 Signaling and protein kinases that are involved in ciliary disassembly
- 14.9 Conclusions and perspectives
- Abbreviations
- References
- Chapter 15. Deciliation
- Abstract
- 15.1 Introduction
- 15.2 Deciliation: key tool of ciliary research
- 15.3 Why do cells deciliate?
- 15.4 Signaling pathways Implicated in deciliation
- 15.5 The mechanism of ciliary severing
- 15.6 Deciliation and the cell cycle
- 15.7 Summary and thoughts for future directions
- References
- Chapter 16. The eyespot and behavioral light responses
- Abstract
- 16.1 Introduction
- 16.2 Methods for behavioral analysis
- 16.3 The directional antenna
- 16.4 Signal generation, propagation and ciliary responses
- 16.5 Homeostatic regulation of phototactic sensitivity
- References
- Chapter 17. Ciliary adhesion and cilium-generated signaling during fertilization in Chlamydomonas
- Abstract
- 17.1 Introduction
- 17.2 Ciliary adhesion
- 17.3 Cilium-generated signaling
- 17.4 Evolutionary perspectives
- References
- Chapter 18. The cellular cytoskeleton
- Abstract
- 18.1 Introduction: Why study the cytoskeleton in Chlamydomonas?
- 18.2 Microtubules
- 18.3 Actin
- 18.4 Septin
- 18.5 Perspectives
- Acknowlegdments
- References
- Further reading
- Index
- Edition: 3
- Published: February 15, 2023
- Imprint: Academic Press
- No. of pages: 470
- Language: English
- Hardback ISBN: 9780128225080
- eBook ISBN: 9780128225097
SD
Susan Dutcher
Susan K. Dutcher is a professor and the former director of the McDonnell Genome Institute at Washington University School of Medicine. She earned her PhD at the University of Washington in Seattle, where her thesis work focused on the role of cell division cycle genes in karyogamy in S. cerevisiae with Dr. Leland Hartwell. In 1980, she began work on Chlamydomonas basal bodies and cilia at Rockefeller University. She was a faculty member at the University of Colorado, Boulder from 1983 to 1999. Her lab investigates the assembly and function of basal bodies/centrioles and cilia using genetics, biochemistry, microscopy, and computational biology in Chlamydomonas as well as human tissue culture cells. Key questions addressed in her research are how these microtubule-based structures are assembled, how they are regulated with respect to the cell cycle, and how they function and influence cellular biology, development, and human health. Stemming from the lab’s comparative genomics work in 2004, the discovery of many cilia and centriole-based diseases has illustrated the incredible breath of roles that these organelles play in human health. She is a fellow of the American Academy of Arts and Sciences, the American Society of Cell Biology, and the American Association for the Advancement of Science. She has had key collaborations with numerous scientists.
Affiliations and expertise
Professor, McDonnell Genome Institute, Washington University School of Medicine, USARead The Chlamydomonas Sourcebook on ScienceDirect