Techniques and Protocols in Molecular Biology
- 1st Edition - September 1, 2025
- Imprint: Academic Press
- Author: Buddhi Prakash Jain
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 3 - 1 4 1 6 0 - 7
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 4 1 6 1 - 4
Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different… Read more
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Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique including isolation techniques for DNA and RNA, gene cloning, PCR, ELISA, and cell culture. These protocols include a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves.
This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work.
• Provides step-by-step instructions to guide researchers in carrying out a range of molecular biology techniques
• Includes details of instrumentation, reagents, and buffers necessary for each experiment
• Features diagrams to help illustrate the procedures and techniques covered
• Includes details of instrumentation, reagents, and buffers necessary for each experiment
• Features diagrams to help illustrate the procedures and techniques covered
Researchers and lab technicians in the field of molecular biology, genomics and cell biology, Postgraduate and doctoral students in molecular biology and related areas
1. Isolation of Genomic DNA: From animal cells, tissues, plant cell, and bacteria
2. Isolation of RNA
3. PCR (Polymerase Chain reaction)
4. Real-time (Quantitative) PCR
5. Electrophoresis
6. 3' RACE
7. 5'RACE
8. Isolation of Plasmid
9. Competent cell preparation
10. Gene Cloning
11. End labelling of DNA
12. SDS and NATIVE PAGE
13. Western Blotting
14. EMSA (Electrophoretic Mobility Shift Assay)
15. Silver staining
16. Cell Culture
17. MTT assay
18. Lactate Dehydrogenase assay
19. Luciferace assay
20. Co-IP
21. Protein purification
22. Sub cellular fractionation
23. DNA foot printing
24. Chromatin Immunoprecipitation
25. Fluorescent activated cell sorting (FACS)
26. Basics of Bioinformatics
27. GST Pulldown assay
28. Fluorescence In situ Hybridization
29. Yeast Two Hybrid Assay
30. ELISA (Enzyme Linked Immuno Sorbent Assay)
2. Isolation of RNA
3. PCR (Polymerase Chain reaction)
4. Real-time (Quantitative) PCR
5. Electrophoresis
6. 3' RACE
7. 5'RACE
8. Isolation of Plasmid
9. Competent cell preparation
10. Gene Cloning
11. End labelling of DNA
12. SDS and NATIVE PAGE
13. Western Blotting
14. EMSA (Electrophoretic Mobility Shift Assay)
15. Silver staining
16. Cell Culture
17. MTT assay
18. Lactate Dehydrogenase assay
19. Luciferace assay
20. Co-IP
21. Protein purification
22. Sub cellular fractionation
23. DNA foot printing
24. Chromatin Immunoprecipitation
25. Fluorescent activated cell sorting (FACS)
26. Basics of Bioinformatics
27. GST Pulldown assay
28. Fluorescence In situ Hybridization
29. Yeast Two Hybrid Assay
30. ELISA (Enzyme Linked Immuno Sorbent Assay)
- Edition: 1
- Published: September 1, 2025
- Imprint: Academic Press
- Language: English
BP
Buddhi Prakash Jain
Dr. Buddhi Prakash Jain is an Assistant Professor at the Department of Zoology, Mahatma Gandhi Central University, Motihari, Bihar, India. He completed his doctoral studies at the School of Life Sciences, Jawaharlal Nehru University (JNU) New Delhi, India. His research interests include gene expression regulation and endoplasmic reticulum stress in cancer. He is the recipient of several awards including a gold medal in Master Programme (M.Sc.), EMBO travel award, DST (Department of Science and Technology) Govt of India, Young Scientist travel award, and has published several papers in reputed international journals and a number of books and book chapters.
Affiliations and expertise
Assistant Professor, Department of Zoology, School of Life Sciences, Mahatma Ghandi Central University, Motihari, Bihar, India