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Techniques and Protocols in Molecular Biology
- 1st Edition - May 1, 2025
- Author: Buddhi Prakash Jain
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 3 - 1 4 1 6 0 - 7
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 4 1 6 1 - 4
Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different… Read more
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Request a sales quoteTechniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique, including isolation techniques for DNA and RNA, gene cloning, PCR, DNA sequencing, ELISA and cell culture. These protocols all includes a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves.
This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work.
- Provides step-by-step instructions to guide researchers in carrying out a range of molecular biology techniques
- Includes details of instrumentation, reagents and buffers necessary for each experiment
- Features diagrams to help illustrate the procedures and techniques covered
Researchers and lab technicians in the field of molecular biology, genomics and cell biology, Postgraduate and doctoral students in molecular biology and related areas
1. Isolation of Genomic DNA: From animal cells, tissues, plant cell, and bacteria
2. Isolation of Plasmid
3. Isolation of RNA
4. PCR (Polymerase Chain reaction)
5. Real-time (Quantitative) PCR
6. Electrophoresis
7. Capillary electrophoresis
8. Purification of Nucleic acid
9. Southern blotting
10. Northern blotting
11. ELISA (Enzyme Linked Immuno Sorbent Assay)
12. EMSA (Electrophoretic Mobility Shift Assay)
13. Gene Cloning
14. End labelling of DNA
15. DNA sequencing
16. RNA sequencing
17. Microarray
18. Generation of Genomic and cDNA library.
19. Western Blotting
20. Cell Culture
21. Fluorescent activated cell sorting (FACS)
22. DNA microarray
23. DNA foot printing
24. Chromatin Immunoprecipitation
25. Basics of Bioinformatics
26. Isolation of Proteins
27. Protein purification
28. Fluorescence In situ Hybridization
2. Isolation of Plasmid
3. Isolation of RNA
4. PCR (Polymerase Chain reaction)
5. Real-time (Quantitative) PCR
6. Electrophoresis
7. Capillary electrophoresis
8. Purification of Nucleic acid
9. Southern blotting
10. Northern blotting
11. ELISA (Enzyme Linked Immuno Sorbent Assay)
12. EMSA (Electrophoretic Mobility Shift Assay)
13. Gene Cloning
14. End labelling of DNA
15. DNA sequencing
16. RNA sequencing
17. Microarray
18. Generation of Genomic and cDNA library.
19. Western Blotting
20. Cell Culture
21. Fluorescent activated cell sorting (FACS)
22. DNA microarray
23. DNA foot printing
24. Chromatin Immunoprecipitation
25. Basics of Bioinformatics
26. Isolation of Proteins
27. Protein purification
28. Fluorescence In situ Hybridization
- No. of pages: 250
- Language: English
- Edition: 1
- Published: May 1, 2025
- Imprint: Academic Press
- Paperback ISBN: 9780443141607
- eBook ISBN: 9780443141614
BP
Buddhi Prakash Jain
Dr. Buddhi Prakash Jain is an Assistant Professor at the Department of Zoology, Mahatma Gandhi Central University, Motihari, Bihar, India. He completed his doctoral studies at the School of Life Sciences, Jawaharlal Nehru University (JNU) New Delhi, India. His research interests include gene expression regulation and endoplasmic reticulum stress in cancer. He is the recipient of several awards including a gold medal in Master Programme (M.Sc.), EMBO travel award, DST (Department of Science and Technology) Govt of India, Young Scientist travel award, and has published several papers in reputed international journals and a number of books and book chapters.
Affiliations and expertise
Assistant Professor, Department of Zoology, School of Life Sciences, Mahatma Gandhi Central University, Motihari, Bihar, India