Targeted Genome Engineering via CRISPR/Cas9 in Plants

1st Edition - July 18, 2024
Imprint: Academic Press
Editor: Alvina Gul
Language: English
Paperback ISBN:
9 7 8 - 0 - 4 4 3 - 2 6 6 1 4 - 0
eBook ISBN:
9 7 8 - 0 - 4 4 3 - 2 6 6 1 5 - 7

Targeted Genome Engineering via CRISPR/Cas9 in Plants provides in-depth insights into the use of the emerging “CRISPR/Cas9” technology for precise genome editing. This tech… Read more

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Targeted Genome Engineering via CRISPR/Cas9 in Plants provides in-depth insights into the use of the emerging “CRISPR/Cas9” technology for precise genome editing. This technology has revolutionized plant science research particularly for crop improvement owing to its simplicity and efficiency. The book provides a wide range of CRISPR/Cas9 gene editing techniques for a variety of plants. Chapters include the latest applications of CRISPR/Cas9 system in connection with abiotic stresses, biotic stresses, biofortification, yield improvement, disease modelling and prognosis and molecular diagnosis. Targeted Genome Engineering via CRISPR/Cas9 in Plants also evaluates various regulatory and ethical aspects that must be considered when implementing the CRISPR/Cas9 approach.

This book is a valuable resource for professionals and researchers, as it provides effective CRISPR/Cas9-based strategies for sustainable agriculture and treatment of various diseases.

Cover image
Title page
Table of Contents
Copyright
Contributors
Preface
Chapter 1 History and discovery of genome engineering
Abstract
1 Introduction
2 Genome editing technologies
3 Historical perspectives
4 Pharmacogenetics and pharmacogenomics
5 Application of genome engineering
6 Conclusion and future perspective of genome engineering
References
Chapter 2 A new insight into genome editing: CRIPR-Cas system in bacteria from evolution to application
Abstract
1 Introduction
2 Techniques of genome editing
3 Evolutionary significance of CRISPR-Cas system
4 Classification of CRISPR-Cas system
5 Functional diversity of CRISPR-Cas system
6 Mechanism of CRISPR-Cas9-mediated editing
7 Applications in infectious disease treatment
8 Drawbacks of CRISPR-Cas system
9 Conclusion
References
Chapter 3 CRISPR-Cas9-mediated genome editing in fungi: Current scenario and future implications in agriculture, health, and industry
Abstract
1 Introduction
2 CRISPR-Cas9 in the fungi and its implication in the field of agriculture
3 Medical applications of CRISPR-Cas9 in fungi
4 Industrial applications of CRISPR-Cas9 in fungi
5 Conclusion
References
Chapter 4 High-throughput genome engineering: Use of CRISPR/Cas9 in Arabidopsis thaliana modification
Abstract
1 Introduction
2 Clustered regularly interspaced short palindromic repeats
3 Arabidopsis thaliana: A model organism for research
4 Conclusion
References
Chapter 5 Genome engineering in banana
Abstract
1 Introduction
2 Banana cultivars
3 Conventional propagation methods in banana
4 Methods for genetic engineering in banana
5 Applications of genome engineering in banana
6 Nano-biotechnology for banana plants
7 Conclusion and future prospects
References
Chapter 6 Genetic engineering in tomato
Abstract
1 Introduction
2 Genetically modified Tomato
3 Abiotic stress resistance
4 Genetic engineering (GE) applications for improved stress tolerance
5 Genetic modification of specific genes in tomato
6 Biotic resistance
7 Improved aroma and flavor
8 Edible vaccines from tomato
9 Conclusion
References
Chapter 7 Genetic engineering techniques in potato
Abstract
1 Introduction
2 Potato breeding and genomic selection
3 Improving potato (Solanum tuberosum L.) through genome editing
4 Genome editing: A paradigm in potato breeding
5 Next-generation phenotyping of potato
6 Perspectives
References
Chapter 8 Genomic engineering in peanut
Abstract
1 Introduction
2 Peanut
3 Genomic engineering in peanut
4 Stresses
5 Methods of peanut transformation
6 Development of agronomic traits
7 Segregation ratio
8 Biofortification and peanut genes
9 GM peanut holds for global security and safety
References
Chapter 9 Genome engineering in soybean
Abstract
1 Introduction
2 Genome engineering in soybean
3 Genome editing of soybean using ZFNs and TALENs
4 Genome editing of soybean using CRISPR/Cas9 technology
5 Improvement in soybean through genome editing
6 Soybean improvement against abiotic stress
7 Soybean improvement against biotic stress
8 Soybean improvement for agronomic traits
9 Future prospects of soybean genome editing
10 Conclusion
References
Chapter 10 Genome editing techniques and mechanism of canola editing
Abstract
1 Canola: Origin and distribution
2 An introduction to genetic engineering
3 Genome editing techniques
4 Cas9: An RNA-guided nuclease for genome editing
5 CRISPR-Cas9-based genome editing
6 Comparison of CRISPR-Cas9 with TALENs and ZFNs
7 Applications of CRISPR-Cas9 in plant biology and biotechnology
8 Model crop plants
9 Genetic engineering in canola
10 Conclusion
References
Chapter 11 Genome engineering in wheat against drought stress
Abstract
1 Introduction
2 The wheat genome
3 Effect of drought on yield grain
4 Yield stability under drought stress
5 Technologies for drought tolerance in wheat
6 Comparison of gene transformation techniques regarding wheat
7 Role of transcriptional regulators in drought conditions
8 Site-specific nucleases: Overview
9 CRISPR/Cas system
10 Conclusion and future prospectives
References
Chapter 12 Genome engineering in maize using CRISPR/CAS9 system
Abstract
1 Introduction
2 Genome engineering in maize
3 Genome engineering in maize by CRISPR/CAS9 system
4 Conclusion
References
Chapter 13 Genome engineering in barley
Abstract
1 Introduction
2 An extensive study of barley as an agricultural crop
3 Cas endonuclease technology in barley genetic engineering
4 Study of the function of HvPAPhy-a gene by using CRISPR/Cas9
5 Phenotypic variations introduced by Cas-endonuclease technology
6 Defense mechanisms in genetically engineered barley
7 Transgenic barley with improved agricultural traits
8 Functional genomic approaches in barley
9 Genetics and its role in barley
10 Conclusions
References
Chapter 14 Genome engineering in rice
Abstract
1 Introduction
2 Biotic stress tolerance
3 Abiotic stress tolerance
4 Yield improvement by genome editing
5 Conclusion
References
Chapter 15 Genome engineering in sorghum
Abstract
1 Introduction
2 Basic genetic approaches for yield improvement in sorghum
3 Modern genetic tools for genome engineering in sorghum
4 Applications of genome engineering in sorghum
5 Conclusion
References
Chapter 16 Genome engineering in cotton (Gossypium hirsutum)
Abstract
1 Introduction
2 The new era of genome editing
3 Mechanism of gene editing via the CRISPR/Cas9 systems
4 Applications of genome engineering in cotton
5 Conclusions
References
Chapter 17 Genome engineering in sugarcane
Abstract
1 Introduction
2 Yield improvement in sugarcane
3 Genome engineering in sugarcane
4 Applications of genome engineering in sugarcane
5 Challenges
6 Conclusion
References
Chapter 18 Genome modifications in cassava
Abstract
1 Introduction
2 Various biotechnology methods or tools in improving the production of cassava crop
3 Disease-resistant cassava using genome editing technologies
4 Cassava’s starch modifications
5 Biofortification of cassava for better nutrition
6 Improving storage, root production, and postharvest storage
7 Future perspective
References
Chapter 19 Genome engineering in tobacco
Abstract
1 Gene silencing: Promising approaches to better understand and control tobacco pathogens
2 Zinc finger nuclease
3 TALENs: The breakthrough of the year 2011
4 Basic mechanism of HR
5 Basic mechanism of CRISPR/Cas9
6 Homologous recombination in tobacco via CRISPR/Cas9
7 Viruses as vectors for genome engineering in tobacco
8 Virus-based vectors for multiplexed gene editing
9 Nicotine resistance in tobacco
10 CRISPR/Cas gRNA-mediated genome editing
11 Low nicotine gene edited tobacco
12 Knockout of NtFAD2 by CRISPR/Cas9 to design high oleic acid in tobacco plant
13 Cas9 system for tobacco breeding
14 CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum
15 Cas9-induced mutagenesis of NtPDS and NtPDR6 in tobacco protoplast
16 Editing of the Cyp82E4-nnd in tobacco plants
17 CRISPR/Cpf1 gene editing in plants
18 Targeted mutagenesis in tobacco
19 Nanotechnology for genetic engineering
20 Conclusion
References
Chapter 20 Industrial applications of genetic engineering
Abstract
1 Introduction to genetic engineering and industries
2 Recombinant DNA technology and industrial enzymes
3 Genetic engineering applications in healthcare and pharma industries
4 Genetic engineering applications in agriculture industry
5 Genetic engineering applications in food industry
6 Genetic engineering applications in fermentation industry
7 Genetic engineering applications in biofuel industry
8 Genetic engineering applications in chemical and textile industry
9 Conclusion
References
Chapter 21 The economics of genetically modified crop (GMO) adoption
Abstract
1 Introduction
2 The worldwide need for genetically modified crops: Global food insecurity
3 Cost and risks for developing and adopting genetic modification of crops
4 Effects of genetically modified crops on production costs and income
5 Markets for genetically modified crops and consumers’ willingness to pay
6 Overall economic impact of genetically modified crops: The secluded impact
7 Concluding remarks
References
Chapter 22 Genome engineering and bioethics
Abstract
1 Introduction
2 Major threats
3 Secular perspective
4 Religious perspective
5 Biodiversity
6 Revival of extinct species
7 End of nominal equality in life
8 Cultural impact
9 Bioethical issues and genome-editing techniques
10 Conclusion
References
Index

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Targeted Genome Engineering via CRISPR/Cas9 in Plants

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Alvina Gul

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