Structural and Resonance Techniques in Biological Research

List of Contributors

Series Introduction

Preface


1. Nuclear Magnetic Resonance

I. Introduction

II. Introduction to Nuclear Magnetic Resonance

III. Theory

IV. Experimental Considerations

V. Applications

VI. Concluding Remarks

Appendix

References


2. Electron Spin Resonance

I. Introduction

II. Principles of Electron Spin Resonance

III. Establishing and Detecting Electron Spin Resonance

IV. Applications of Direct (Continuous-Wave) ESR

V. Resonance as a Perturbation

VI. An ESR Perspective

Appendix A. ESR Hardware, Glassware, and Supplies

Appendix B. ESR Software

Appendix C. Calibration of ESR Spectra

Bibliography

References


3. Mössbauer Spectroscopy

I. Introduction

II. Principles of Mössbauer Spectroscopy

III. Experimental Techniques

IV. Applications to Isolated Biomolecules

V. Applications to Molecular Systems

VI. Applications to Tissue

VII. Applications to Whole or Part Organisms: Uptake and Metabolism

VIII. Measurement of Vibration and Movement

IX. Conclusions

References


4. X-Ray Absorption Spectroscopy

I. Introduction

II. Theory

III. Experimental Considerations

IV. EXAFS as a Structural Technique in Biology

V. Conclusion

References


5. Macromolecular Crystallography

I. Introduction

II. Crystallization of Macromolecules

III. X-Ray Diffraction from Crystals

IV. Phase Determination

V. Heavy-Atom Derivatives

VI. The Electron-Density Map

VII. New Directions

Appendix A. The Basic Mathematics of Crystallography

Appendix B. A Specific Strategy for Protein Crystallization

Appendix C. A Crystallographic Paper Primer

Appendix D. Suggestions for Further Reading

References


6. Small-Angle X-Ray Scattering and Diffraction

I. Introduction

II. X-Ray Scattering and Diffraction

III. X-Ray Technology

IV. Biological Applications

V. Summary

References

Index