Steroids in the Laboratory and Clinical Practice
- 1st Edition - August 3, 2023
- Latest edition
- Author: John William Honour
- Language: English
Steroids in the Laboratory and Clinical Practice covers both basic chemistry and therapeutic application of steroids in a single source. The comprehensive reference addres… Read more
Steroids in the Laboratory and Clinical Practice covers both basic chemistry and therapeutic application of steroids in a single source. The comprehensive reference addresses the specificity of steroid determinations to clarify confusion arising from the laboratory results. The book covers important advancements in the field and is a valuable addition in the literature addressing all existing knowledge gaps. This is a must have reference for pathologists, laboratorians, endocrinologists, analytical/clinical chemists and biochemists.
- Addresses the normal production of steroids and concentrations found in biological fluids and tissues
- Presents the changes in steroid concentrations at life events as reference points for clinical investigations
- Reviews the genetic disorders of steroids in relation to specific enzyme changes and clinical presentation
Researchers, practitioners, endocrinologists, pathologists/laboratorians, analytical chemists, biochemists and chemical pathologists
Part 1 steroid biochemistry and endocrinology
1.1. Chemistry
1.2. Sources of steroids
1.3. Biosynthesis
1.4. Regulation
1.5. Steroids in blood and tissues
1.6. Recognition of steroids
1.7. Physiological effects
1.8. Breakdown and clearance
PART 2 Laboratory investigations
2.1. Samples
2.2 Sample preparation
2.3 Purification of extracts
2.4 Purification of steroids
2.5 Concentrations of steroids in blood
2.6. Concentrations of steroids in urine, other biological fluids and tissues
2.7 Confirmatory tests
Part 3. Clinical investigations
3.1 Defects of cortisol
3.1.1 Hypercortisolism
3.1.2 Hypocortisolism
3.2. Defects of aldosterone
3.2.1. Hyperaldosteronism
3.2.2. Hypoaldosteronism
3.3. Defects of gonadal steroids
3.3.1. Hypergonadism
3.3.2. Hypogonadism
- Edition: 1
- Latest edition
- Published: August 3, 2023
- Language: English
JH
John William Honour
Dr. Honour has 45 years of experience in analyzing steroids mainly by gas chromatography and mass spectrometry and applying these results to the investigation of patients with endocrine disorders. His work has included the diagnosis of inherited disorders of blood pressure, sex determination, infertility, osteoporosis, and salt balance in the body. He has long had an interest in the ability of bacteria to change steroids in the body and has used bacteria in test-tubes to achieve some changes in steroid structure which could not be easily achieved with chemicals. Several research projects have examined the safety of inhaled steroids with respect to effects on the hypothalamic-pituitary-adrenal axis.
Dr. Honour is an Honorary Senior Research Associate at the Institute for Women's Health at University College London and formerly a Consultant Clinical Scientist in Clinical Biochemistry at UCLH and Head of the specialist service for Steroid Endocrinology. The steroid laboratory maintains strong links with the clinical endocrinologists at UCL and the Hospital for Children at Great Ormond Street. Research in the unit covered the validation and use of mass spectrometry in steroid analysis for disorders of sex determination, adrenal diseases, polycystic ovary syndrome, safety of corticosteroids in treatment of asthma, links between birthweight and cardiovascular risk, and the enterohepatic circulation of steroids.
Dr. Honour has more than 170 refereed publications and 19 book chapters covering a range of analytical, genetic and clinical aspects of steroids. He presented 175 papers at scientific conferences. He was scientific adviser to UKNEQAS for immunoassays, steroid accuracy and pediatric investigations. He served on two working parties for the European Society for Pediatric Endocrinology with the Lawson Wilkins Pediatric Society that produced guidelines on congenital adrenal hyperplasia and newborn screening. An EQA scheme for urinary steroids was operated from UCL globally with the collaboration of the Dutch Foundation for Quality in Clinical Chemistry (SKML). Dr Honour has maintained an interest in the detection of abuse of anabolic steroids in sport and has acted as an expert witness in cases of drug abuse in sport. He is an Associate Editor of the Annals of Clinical Biochemistry.
Affiliations and expertise
Senior Research Associate, Institute for Women’s Health, University College London, UKRead Steroids in the Laboratory and Clinical Practice on ScienceDirect