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Robotic Cell Manipulation

1st Edition - May 31, 2022

Author: Dong Sun

Paperback ISBN:

9 7 8 - 0 - 3 2 3 - 8 5 2 5 9 - 3

eBook ISBN:

9 7 8 - 0 - 3 2 3 - 8 5 2 6 0 - 9

Robotic Cell Manipulation introduces up-to-date research to realize this new theme of medical robotics. The book is organized in three levels: operation tools (e.g., optical… Read more

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Robotic Cell Manipulation introduces up-to-date research to realize this new theme of medical robotics. The book is organized in three levels: operation tools (e.g., optical tweezers, microneedles, dielectrophoresis, electromagnetic devices, and microfluidic chips), manipulation types (e.g., microinjection, transportation, rotation fusion, adhesion, separation, etc.), and potential medical applications (e.g., micro-surgery, biopsy, gene editing, cancer treatment, cell-cell interactions, etc.). The technology involves different fields such as robotics, automation, imaging, microfluidics, mechanics, materials, biology and medical sciences. The book provides systematic knowledge on the subject, covering a wide range of basic concepts, theories, methodology, experiments, case studies and potential medical applications.

It will enable readers to promptly conduct a systematic review of research and become an essential reference for many new and experienced researchers entering this unique field.

Cover image
Title page
Table of Contents
Copyright
Dedication
Preface
Acknowledgments
1. Introduction
1.1. Overview of robot-facilitated cell manipulation
1.2. Outline of the book
1.3. Conclusions
2. Cell manipulation tools
2.1. Introduction
2.2. Microneedle
2.3. Optical tweezers
2.4. Electrokinetics
2.5. Magnetic manipulator
2.6. Atomic force microscopy
2.7. Imaging for intracellular manipulation
2.8. Conclusions
3. Robotic cell injection
3.1. Introduction
3.2. Robot-assisted cell microinjection system with microneedles
3.3. Hybrid position and force control for automated batch injection of cells
3.4. Universal piezo-driven ultrasonic cell microinjection
3.5. Automated microinjection for small human cells
3.6. Automated high-productivity microinjection for adherent cells
3.7. Single-cell transfection through precise microinjection with quantitatively controlled injection volumes
3.8. Characterization of mechanical properties of cells through microinjection
3.9. Conclusions
4. Cell stretching and compression
4.1. Introduction
4.2. Cell stretching with optical tweezers
4.3. Probing cell biophysical behavior based on actin cytoskeleton modeling of cells and stretching manipulation with optical tweezers
4.4. Cell stretching with dielectrophoresis technology
4.5. Cell compression under mechanical confinement
4.6. Magnet-based cell deformation for intracellular delivery
4.7. Conclusions
5. Cell transport with optical tweezers
5.1. Introduction
5.2. Basic theory and methods
5.3. Motion and path planning for automatic cell transportation
5.4. Unified motion control design
5.5. Multiple cell transportation for cell pairing
5.6. Cell transportation for multiprocessing automation tasks
5.7. Conclusions
6. Cell rotation
6.1. Introduction
6.2. Automated in-plane rotation of cells using a robot tweezers manipulation system
6.3. Automated out-of-plane rotation of cells using a robot tweezers manipulation system
6.4. Conclusions
7. Three-dimensional image reconstruction and intracellular surgery
7.1. Introduction
7.2. 3D image reconstruction
7.3. Robot-assisted intracellular delivery with 3D image reconstruction information
7.4. Conclusions
8. Cell sorting and separation
8.1. Introduction
8.2. Cell sorting using combined optical tweezers and microfluidic chip technology
8.3. Cell isolation and deposition
8.4. A simplified sheathless cell separation approach
8.5. Conclusions
9. Cell stimulation and migration control
9.1. Introduction
9.2. Dynamic model of chemoattractant-induced cell migration
9.3. Cell migration control using a stimulus-induced robotic manipulation system
9.4. Electrical stimulation based on calcium spike patterns of MSCs to improve osteogenic differentiation
9.5. Conclusions
10. Cell patterning
10.1. Introduction
10.2. Cell patterning with robotically controlled optical tweezers
10.3. Cell patterning using a dielectrophoresis-based multilayer scaffold structure
10.4. Cell patterning using a gravitational sedimentation-based microfluidic approach
10.5. Conclusions
11. Cell adhesion
11.1. Introduction
11.2. Manipulating cell adhesion with optical tweezers
11.3. Adhesion-mediated cell–cell interaction
11.4. A case study of cell adhesion characterization
11.5. Conclusions
12. Cell fusion
12.1. Introduction
12.2. Laser-induced fusion with optical tweezers
12.3. Cell fusion with combined optical tweezers and microwell array technology
12.4. A case study of transforming liver cancer cells into tumor initiating-like cells by cell fusion
12.5. Conclusions
13. Cell navigation and delivery in vivo
13.1. Introduction
13.2. In vivo navigation of single cells with an optical tweezers-based manipulator
13.3. Magnetic microrobot for carrying and delivering cells in vivo
13.4. Precise delivery of stem cells for cancer therapy using magnet-driven and image-guided degradable microrobots
13.5. Conclusions
14. Organelle biopsy and gene editing of single cells
14.1. Introduction
14.2. Automated organelle biopsy of single cells using microneedles
14.3. Gene-delivery approaches for MSC function improvement
14.4. Automated optical tweezers manipulation for mitochondrial transfer
14.5. Conclusions
15. Summary
15.1. Operation tools for cell manipulations
15.2. Cell manipulation types
15.3. Potential medical applications
Index