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Revealing Uncharted Biology With Single Cell Multiplex Proteomic Technologies
Applications, Experimental Design, Methods, and Protocols
1st Edition - June 1, 2024
Editor: Wendy Fantl
Paperback ISBN:
9 7 8 - 0 - 1 2 - 8 2 2 2 1 3 - 3
eBook ISBN:
9 7 8 - 0 - 1 2 - 8 2 2 2 1 4 - 0
Revealing Unchartered Biology with Single Intact Cells: Case Studies explores the path to research success, key projects, the role of techniques, the selection process, other… Read more
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Revealing Unchartered Biology with Single Intact Cells: Case Studies explores the path to research success, key projects, the role of techniques, the selection process, other alternatives considered, what other paths have led to dead ends, detailed protocols followed, and how the analysis of generated data allowed researchers to visualize unchartered biology. Focusing on the research journey that led to the publication of each article, the book's editors interviewed the researchers on the use of the Multiplex Single Cell technique and how it helped hone in on the biological quest. These methods can be expanded to a wide variety of research objectives.In conclusion to each chapter, the authors critically review their process and provide suggestions of improvement or alternate techniques that could be employed. This book is the ideal reference for researchers new to the world of single-cell multiplex techniques. The discussion on failures encountered along the research path provides insights on how to avoid repeating the same errors.
- Provides insights into the path to success of key research articles based on Multiplex Single-Cell analysis techniques results
- Contains detailed method information
- Discusses strengths and limitations of techniques applied to each research domain covered
- Includes discussions on the failures encountered along the research path and how to avoid them
I. Coordinated cellular neighborhoods orchestrate antitumoral immunity at the colorectal cancer invasive front by Schürch et al.
1. Revealing Unchartered Biology
2. Research methods and protocols used in the experiment
3. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research
II. Commonly Occurring Cell Subsets in High-Grade Serous Ovarian Tumors Identified by Single-Cell Mass Cytometry by Gonzalez et al.
4. Revealing Unchartered Biology
5. Research methods and protocols used in the experiment
6. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research
III. Mass cytometry of Hodgkin lymphoma reveals a CD41 regulatory T-cell–rich and exhausted T-effector microenvironment, by Cader et al.
7. Revealing Unchartered Biology
8. Research methods and protocols used in the experiment
9. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research
IV. Bystander CD8+ T cells are abundant and phenotypically distinct in human tumour infiltrates by Simoni et al.
10. Revealing Unchartered Biology
11. Research methods and protocols used in the experiment
12. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research
V. High-resolution myogenic lineage mapping by single-cell mass cytometry by Porpiglia et al.
13. Revealing Unchartered Biology
14. Research methods and protocols used in the experiment
15. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research
VI. Single-cell mass cytometry reveals distinct populations of brain myeloid cells in mouse neuroinflammation and neurodegeneration models, by Ajami et al.
16. Revealing Unchartered Biology
17. Research methods and protocols used in the experiment
18. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research
VII. Conclusion
- No. of pages: 300
- Language: English
- Edition: 1
- Published: June 1, 2024
- Imprint: Academic Press
- Paperback ISBN: 9780128222133
- eBook ISBN: 9780128222140
WF
Wendy Fantl
Dr. Wendy Fantl is an assistant professor in the Department of Urology and by-courtesy appointment in Obstetrics and Gynecology Stanford University (CA, USA). Wendy received her BA in Biochemistry from the University of York (UK) and PhD in Protein Biochemistry from Rockefeller University (NY, USA) before becoming a post-doc and adjunct assistant professor at the University of California, San Francisco (CA, USA). Wendy has 16 years of experience working in Biotechnology and Pharmaceutical industries. Wendy’s passion is in the mechanistic understanding of intracellular signaling pathways, DNA damage and the host immune system and their role in malignancies with an “eye on the prize” of a clinically translational endpoint. Her current interests are to apply multi-parametric single-cell technology platforms to identify disease-causing cell subpopulations in ovarian cancer and other malignancies.
Affiliations and expertise
Assistant Professor, Department of Urology and by-courtesy appointment in Obstetrics and Gynecology Stanford University, CA, USA