Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part B
- 1st Edition, Volume 180 - April 29, 2021
- Imprint: Academic Press
- Editor: Vijai Singh
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 8 5 2 9 7 - 5
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 8 5 2 9 8 - 2
Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part B, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Ca… Read more
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Request a sales quoteReprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part B, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. It is a simple, rapid, precise, and cost-effective tool for genome editing and regulation of a wide range of organisms. It has gained scientific and public attention worldwide. This volume mainly covers insect cell line, protozoans, zebrafish, drosophila, CRISPRi, patents as well as technology transfer, and many more. This book is a key source of information available in a single volume. This book will be useful for not only beginners in genome engineering, but also students, researchers, scientists, policymakers, and stakeholders interested in harnessing the potential of reprogramming of the genomes in several areas.
- Offers basic understanding and a clear picture of genome editing CRISPR-Cas systems in different organisms
- Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for insect cell line, protozoans, zebrafish, drosophila, and many more
- Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, molecular biology and many more
Beginners in area of genome engineering area, but also researchers, students, scientists, clinicians, stakeholders, policy makers and practitioners and many more
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Preface
- Chapter One: Development of insect cell line using CRISPR technology
- Abstract
- 1: Introduction
- 2: Advantages of the use of insects in biomedical research
- 3: Mechanism of CRISPR technology
- 4: Cas9
- 5: CRISPR/cas9 technology system on the model and non-model insects
- 6: CRISPR/Cas9 gene editing for Sf9/Sf21 cells and baculovirus genome engineering
- 7: Applications and challenges of the CRISPR/cas9 system in insect cells to study different diseases models
- 8: Conclusion
- Chapter Two: CRISPRing protozoan parasites to better understand the biology of diseases
- Abstract
- 1: Introduction
- 2: CRISPR to study the biology of protozoan parasites
- 3: Limitations to genetically manipulate protozoan parasites
- 4: Advances in the field of CRISPR
- 5: Concluding remarks
- Acknowledgments
- Chapter Three: CRISPR-based genome editing of zebrafish
- Abstract
- 1: Introduction
- 2: CRISPR/Cas9 as genome editing technology
- 3: Genome editing in zebrafish using CRISPR/Cas
- 4: CRISPR/Cas knock-out mechanism
- 5: CRISPR/Cas9 knock-in approaches
- 6: CRISPR/Cas9 mediated base editing
- 7: CRISPR/Cas9 guided transcriptional regulation
- 8: Conclusion and future remarks
- Chapter Four: Using CRISPR-Cas9-based genome engineering tools in Drosophila melanogaster
- Abstract
- 1: Introduction
- 2: CRISPR-Cas9 as a genome engineering tool
- 3: Using CRISPR in Drosophila melanogaster
- 4: Cas9 in Drosophila
- 5: Other Cas enzymes
- 6: Sequence-specific guide RNA
- 7: Screening for mutations
- 8: Drosophila cell lines, a great system for genome wide screens
- 9: Tissue-specific genome engineering using CRISPR-Cas9
- 10: Generating multiple mutations simultaneously using CRISPR
- 11: Homologous recombination
- 12: Homology independent insertions using CRISPR-Cas9
- 13: Generating homozygous mutations by mutagenic chain reaction
- 14: Final thoughts
- Acknowledgments
- Chapter Five: CRISPR interference and its applications
- Abstract
- 1: Introduction
- 2: How CRISPR is reproduced to be utilized for interference (enzymes, machinery, mechanisms)
- 3: Factors affecting the efficiency of CRISPRi-mediated knockdown
- 4: Strengths of CRISPR interference in comparison with other techniques
- 5: Limitations of CRISPR interference in comparison with other techniques
- 6: Comparison between CRISPRi and RNAi
- 7: Comparison between CRISPRi and CRISPR/Cas9
- 8: CRISPR activation (CRISPRa)
- 9: Applications of CRISPRi
- 10: Conclusion and future remarks
- Chapter Six: CRISPR-Cas systems: Challenges and future prospects
- Abstract
- 1: Introduction
- 2: Challenges pertaining to CRISPR-Cas systems
- 3: Conclusion and future prospects
- Acknowledgments
- Chapter Seven: Patents and technology transfer in CRISPR technology
- Abstract
- 1: Introduction
- 2: Current and recent development of CRISPR technology on non-mammalian systems
- 3: Current patent landscape of CRISPR/Cas9 technology in non-mammalian systems
- 4: Comparative patent landscape analysis of CRISPR/Cas9 technologies in non-mammalian systems between developed and developing countries
- 5: Current technology transfer landscape of CRISPR technology in non-mammalian systems
- 6: Success stories: CRISPR technology for non-mammalian systems
- 7: Future advancements and challenges for CRISPR technology
- 8: Conclusion and future remarks
- Index
- Edition: 1
- Volume: 180
- Published: April 29, 2021
- No. of pages (Hardback): 198
- No. of pages (eBook): 198
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780323852975
- eBook ISBN: 9780323852982
VS
Vijai Singh
Dr Vijai Singh is a Professor and Dean (Research & Innovation) at School of Sciences, Indrashil University, Rajpur, Mehsana, Gujarat, India. He was an Associate Professor in the Department of Biosciences, School of Sciences, Indrashil University, Rajpur, Mehsana, Gujarat, India. Prior this this, he was an Assistant Professor in the Department of Biological Sciences and Biotechnology at the Institute of Advanced Research, Gandhinagar, India and also an Assistant Professor in the Department of Biotechnology at the Invertis University, Bareilly, India. Prior to that, he was a Postdoctoral Fellow in the Synthetic Biology Group at the Institute of Systems and Synthetic Biology, Paris, France and School of Energy & Chemical Engineering at the Ulsan National Institute of Science and Technology, Ulsan, South Korea. He received his Ph.D. in Biotechnology from the National Bureau of Fish Genetic Resources, Uttar Pradesh Technical University, Lucknow, India with a research focus on the development of molecular and immunoassays for diagnosis of Aeromonas hydrophila. His research interests are focused on building novel biosynthetic pathways for production of medically and industrially important biomolecules. Additionally, his laboratory is working on CRISPR-Cas9 tools for genome editing. He has more than 11 years of research and teaching experience in synthetic biology, metabolic engineering, bioinformatics, microbiology, and industrial microbiology. He has published 100 articles, 70 chapters, 15 books and 3 patents. He serves as an associate editor, editorial board member, and reviewer of several peer-reviewed journals. He is also a member of the Board of Study and Academic Council of Indrashil University and is the Member Secretary of the Institutional Biosafety Committee (IBSC) at the same University.
Affiliations and expertise
Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, IndiaRead Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part B on ScienceDirect