Recent Advances in iPSC Technology
- 1st Edition - March 31, 2021
- Imprint: Academic Press
- Editor: Alexander Birbrair
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 8 2 2 2 3 1 - 7
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 2 3 2 6 - 0
The series Advances in Stem Cell Biology is a timely and expansive collection of comprehensive information and new discoveries in the field of stem cell biology.Recent Advances… Read more
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Request a sales quoteThe series Advances in Stem Cell Biology is a timely and expansive collection of comprehensive information and new discoveries in the field of stem cell biology.Recent Advances in iPSC Technology, Volume 5 addresses the progress in induced pluripotent stem cells (iPSCs) technologies.Somatic cells can be reprogrammed into iPSCs by the expression of specific transcription factors. These cells are transforming biomedical research in the last 15 years. The volume teaches readers about current advances in the field. This book describes different technologies and strategies to use iPSCs for biological and clinical benefit. In recent years, remarkable progress has been made in the obtention of iPSCs and their differentiation into several cell types, tissues, and organs using state-of-the-art techniques. These advantages facilitated identification of key targets and definition of the molecular basis of several disorders. This volume will cover hot topics in the iPSC field, such as iPSCs for modeling the cardiovascular toxicities of anticancer therapies, iPSC differentiation through the lens of the noncoding genome, modeling of blood–brain barrier with iPSCs, mathematical modeling of iPSCs, iPSCs to study human brain evolution, selfrenewal in iPSCs, differences and similarities between iPSCs and embryonic stem cells, and more.The volume is written for researchers and scientists interested in stem cell therapy, cell biology, regenerative medicine, and organ transplantation and is contributed by world-renowned authors in the field.
- Provides overview of the fast-moving field of induced pluripotent stem cell technology, regenerative medicine, and therapeutics
- Covers the following topics: iPSCs for modeling the cardiovascular toxicities of anticancer therapies, iPSC differentiation through the lens of the non-coding genome, modeling of blood-brain barrier with iPSCs, mathematical modelling of iPSCs, iPSCs to study human brain evolution, self-renewal in iPSCs, differences and similarities between iPSCs and embryonic stem cells, and more
- Contributed by world-renown experts in the field
Researchers and scientists in stem cell therapy, cell biology, regenerative medicine, and organ transplantation. Graduate and undergraduate students in the above fields
- Cover image
- Title page
- Table of Contents
- Advances in Stem Cell Biology
- Copyright
- Dedication
- Contributors
- About the editor
- Preface
- Chapter 1. Modeling the cardiovascular toxicities of anticancer therapies in the era of precision medicine
- Introduction
- Chemotherapy-associated cardiotoxicity
- Modeling cardiotoxicity using iPSCs
- Tissue engineering approaches with iPSCs
- Moving forward: iPSC-based models for precision medicine
- Conclusion
- Chapter 2. Looking at induced pluripotent stem cell (iPSC) differentiation through the lens of the noncoding genome
- Introduction
- iPSC reprogramming from somatic cells
- Differentiation of iPSC into somatic cells and validation of cell types differentiated from iPSC
- lncRNAs as regulators of gene expression
- Epigenetics
- Epigenetic memory of iPSC
- lncRNAs in iPSC differentiation
- Epitranscriptomics
- Commentary on future trends and directions
- Chapter 3. In vitro blood–brain barrier model derived from human iPS cells and its applications
- Introduction
- General explanation about BBB
- How to construct in vitro BBB model from iPSCs
- Application of BBB model
- A commentary on likely future directions
- Chapter 4. The progress in the study of reprogramming to acquire the features of stem cells in iPSCs and cancers
- Introduction
- Similarity between tumorigenesis and the reprogramming process
- Tumor suppression genes, oncogenes, and pluripotency-inducing factors in cancers and iPSCs
- Regulation of cancer progression by hypoxia-inducing (transcription) factors (HIFs)
- Features of CSCs
- Epigenetic modification of the plasticity of CSCs
- Strategies for avoiding tumorigenesis in PSCs and cancers
- Enigmatic functions of JDP2 in tumor proliferation
- Can we generate safe stem cells that do not possess a risk of tumorigenesis?
- Conclusions and perspectives
- Chapter 5. An introduction to the mathematical modeling of iPSCs
- Introduction
- Cell migration as a random walk
- Differential equations
- Agent-based modeling of colonies
- iPSC-specific models
- Discussion and prospects
- Chapter 6. Use of iPSC-derived brain organoids to study human brain evolution
- Introduction
- A short historical background on human brain evolution studies
- Evolution of the central nervous system and neurons
- Genomic comparisons and what can be gained from them
- The use of model organisms in relation to gene expression and cell biology
- An introduction to iPSC-derived brain organoids
- Studies using iPSC-derived brain organoids for human brain evolution
- Future perspectives
- Chapter 7. Self-renewal in induced pluripotent stem cells
- Introduction to induced pluripotent stem cells
- Cell cycle control in pluripotent stem cells
- Cyclin-dependent kinases and cyclins
- Cell cycle regulation in pluripotent stem cells
- Links between pluripotency and cell cycle machinery
- Cell cycle changes during reprogramming to pluripotency
- The onset of differentiation is linked to the cell cycle
- Growth factors implicated in self-renewal
- Fibroblast growth factor
- Transforming growth factor-β
- Wnt/β-catenin signaling
- Other mechanisms involved in the self-renewal of hiPSCs
- Noncoding RNAs
- MicroRNAs
- miRNAs regulate self-renewal via the cell cycle
- miRNAs as suppressors of pluripotency and self-renewal
- lncRNAs
- Telomere maintenance
- Conclusions and outlook
- Chapter 8. Strategies for iPSC expansion: from feeder cells to laminin
- Introduction
- PSC culture systems based on feeder cells
- Matrigel, the first feeder-free system for maintaining pluripotency
- Laminin, a xeno- and feeder-free system for cell expansion
- Scaling-up human PSC expansion
- Conclusion
- Chapter 9. An overview of reprogramming approaches to derive integration-free induced pluripotent stem cells for prospective biomedical applications
- Introduction
- Nonintegrating approaches
- Conclusion
- Chapter 10. Induced pluripotent stem cells versus embryonic stem cells: a comprehensive overview of differences and similarities
- Introduction
- Transcriptional comparison
- Epigenetic comparison
- Proteomic comparison
- Metabolomic comparison
- Conclusion and future trends
- Index
- Edition: 1
- Published: March 31, 2021
- No. of pages (Paperback): 334
- No. of pages (eBook): 334
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9780128222317
- eBook ISBN: 9780128223260
AB
Alexander Birbrair
Dr. Alexander Birbrair received his bachelor’s biomedical degree from Santa Cruz State University in Brazil. He completed his PhD in Neuroscience, in the field of stem cell biology, at the Wake Forest School of Medicine under the mentorship of Osvaldo Delbono. Then, he joined as a postdoc in stem cell biology at Paul Frenette’s laboratory at Albert Einstein School of Medicine in New York. In 2016, he was appointed faculty at Federal University of Minas Gerais in Brazil, where he started his own lab. His laboratory is interested in understanding how the cellular components of different tissues function and control disease progression. His group explores the roles of specific cell populations in the tissue microenvironment by using state-of-the-art techniques. His research is funded by the Serrapilheira Institute, CNPq, CAPES, and FAPEMIG. In 2018, Alexander was elected affiliate member of the Brazilian Academy of Sciences (ABC), and, in 2019, he was elected member of the Global Young Academy (GYA), and in 2021, he was elected affiliate member of The World Academy of Sciences (TWAS). He is the Founding Editor and Editor-in-Chief of Current Tissue Microenvironment Reports, and Associate Editor of Molecular Biotechnology. Alexander also serves in the editorial board of several other international journals: Stem Cell Reviews and Reports, Stem Cell Research, Stem Cells and Development, and Histology and Histopathology.
Affiliations and expertise
Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
Department of Radiology, Columbia University Medical Center, Medical Center, USARead Recent Advances in iPSC Technology on ScienceDirect