Rare-Earth Element Biochemistry: Characterization and Applications of Lanthanide-Binding Biomolecules
- 1st Edition, Volume 651 - April 20, 2021
- Editor: Joseph A. Cotruvo
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 9 1 0 8 5 - 9
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 1 0 8 6 - 6
Rare-Earth Element Biochemistry: Characterization and Applications of Lanthanide-Binding Biomolecules, Volume 651 in the Methods in Enzymology series, continues the legacy of t… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quoteRare-Earth Element Biochemistry: Characterization and Applications of Lanthanide-Binding Biomolecules, Volume 651 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Spectrophotometric methods to probe the solution chemistry of lanthanide complexes with macromolecules, Determination of affinities of lanthanide-binding proteins using chelator-buffered titrations, Electron Paramagnetic Resonance of Lanthanides, Characterization of lanthanoid binding proteins using NMR spectroscopy, Macromolecular crystallography for f-element complex characterization, Infrared spectroscopy probes ion binding geometries, Predicting lanthanide coordination structures in solution with molecular simulation, and much more.
Additional sections cover the Characteristics of Gd(III) spin labels for the study of protein conformations, Lanthanide-based resonance energy transfer biosensors for live-cell applications, Yttrium-86 PET imaging, Aqueous Chemistry of the Smallest Rare Earth: Comprehensive Characterization of Radioactive and Non-radioactive Scandium Complexes for Biological Applications, and In vitro selection and application of lanthanide-dependent DNAzymes.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Preface
- Chapter One: Spectrophotometric methods to probe the solution chemistry of lanthanide complexes with macromolecules
- Abstract
- 1: Introduction
- 2: Ultraviolet–visible–near-infrared spectrophotometry of lanthanide solutions
- 3: Experimental procedure
- 4: Data treatment
- 5: Kd determination of lanthanide-binding macromolecules using UV–vis–NIR spectrophotometry
- 6: Stability of lanthanide–protein complexes assessed by UV–vis–NIR spectrophotometry
- 7: Summary
- Acknowledgments
- Chapter Two: Determination of affinities of lanthanide-binding proteins using chelator-buffered titrations
- Abstract
- 1: Introduction
- 2: Theory
- 3: Experimental procedures
- 4: Applications
- 5: Summary
- Supporting Information
- Acknowledgments
- Chapter Three: Electron paramagnetic resonance of lanthanides
- Abstract
- 1: Introduction
- 2: Detection of EPR signals
- 3: Pulsed measurements of electron spin relaxation
- 4: Overview of T1 of the lanthanides
- 5: Overview of Tm of the lanthanides
- 6: Relaxation times of lanthanide ions in solution at room temperature
- 7: Applications that exploit lanthanide relaxation times
- 8: Summary
- Acknowledgments
- Chapter Four: Characterization of lanthanoid-binding proteins using NMR spectroscopy
- Abstract
- 1: NMR of lanthanoid-containing systems
- 2: Sample preparation
- 3: Acquiring spectra of paramagnetic proteins
- 4: Paramagnetism in structural biology
- 5: Conclusions
- Chapter Five: Macromolecular crystallography for f-element complex characterization
- Abstract
- 1: Introduction
- 2: Lanthanide and actinide crystal growth with macromolecular scaffolds
- 3: Structural analysis
- 4: Summary
- Acknowledgments
- Chapter Six: Infrared spectroscopy probes ion binding geometries
- Abstract
- 1: Introduction
- 2: Properties of infrared spectroscopy
- 3: Application of infrared spectroscopy to proteins
- 4: Analysis and interpretation of data
- 5: Protocol: Collecting FTIR of lanthanide-bound proteins
- 6: Summary
- Acknowledgments
- Chapter Seven: Predicting lanthanide coordination structures in solution with molecular simulation
- Abstract
- 1: Motivation and challenges
- 2: Identify initial structures: Ligand, anion, and solvent molecules around lanthanides
- 3: Refine structures: Concurrent simulation of chemical reactions and dynamics
- 4: A computational protocol to determine the structure of a lanthanide-ligand complex in solution
- 5: Further analysis
- 6: Summary
- Chapter Eight: Characteristics of Gd(III) spin labels for the study of protein conformations
- Abstract
- 1: Introduction
- 2: Zero field splitting (ZFS) and the Gd(III) EPR spectrum
- 3: Gd(III)-Gd(III) PD-EPR
- 4: Conjugation chemistry
- 5: Gd(III) tags
- 6: In-cell applications
- 7: Gd(III)-X DEER
- 8: Outlook
- Acknowledgments
- Chapter Nine: Lanthanide-based resonance energy transfer biosensors for live-cell applications
- Abstract
- 1: Introduction
- 2: LRET biosensor applications and experimental design
- 3: Protocols
- 4: Conclusion
- Chapter Ten: 86Y PET imaging
- Abstract
- 1: Introduction
- 2: Coordination chemistry and nuclear properties of yttrium
- 3: The 86Y/90Y radionuclide pair for theranostic applications
- 4: Importance of accurate dosimetry to prevent kidney damage
- 5: Alternatives to 86Y PET imaging
- 6: 86Y PET imaging to track gadolinium-based contrast agents in vivo
- 7: 86Y-DTPA and 86Y-DOTA: Analytical method development and radiochemistry
- 8: Summary
- Chapter Eleven: Aqueous chemistry of the smallest rare earth: Comprehensive characterization of radioactive and non-radioactive scandium complexes for biological applications
- Abstract
- 1: Introduction
- 2: Production methods of scandium radioisotopes for medicinal applications
- 3: Determination of scandium complex speciation and thermodynamic stability constants
- 4: Variable temperature-mass spectrometry
- 5: Common synthetic, small molecular chelators of scandium
- 6: Scandium binding proteins
- 7: Nuclear magnetic resonance spectroscopy of the Sc-45 nucleus
- 8: Preclinical and clinical applications of scandium radioisotopes
- 9: Summary and outlook
- Acknowledgments
- Chapter Twelve: In vitro selection and application of lanthanide-dependent DNAzymes
- Abstract
- 1: Introduction
- 2: Lanthanide ions binding to DNA
- 3: Lanthanide-dependent RNA-cleaving DNAzymes
- 4: Materials
- 5: In vitro selection of lanthanide-dependent DNAzymes
- 6: Biosensing
- 7: Conclusions
- Acknowledgments
- No. of pages: 412
- Language: English
- Edition: 1
- Volume: 651
- Published: April 20, 2021
- Imprint: Academic Press
- Hardback ISBN: 9780323910859
- eBook ISBN: 9780323910866
JC
Joseph A. Cotruvo
Joseph A. Cotruvo, Jr. is Assistant Professor, Louis Martarano Career Development Professor, Department of Chemistry
Pennsylvania State University, PA, USA
Affiliations and expertise
Assistant Professor, Pennsylvania State University, PA, USARead Rare-Earth Element Biochemistry: Characterization and Applications of Lanthanide-Binding Biomolecules on ScienceDirect