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Rapid Virus Diagnosis
Application of Immunofluorescence
- 2nd Edition - June 28, 2014
- Authors: P. S. Gardner, J. McQuillin
- Language: English
- eBook ISBN:9 7 8 - 1 - 4 8 3 1 - 9 2 1 7 - 8
Rapid Virus Diagnosis: Application of Immunofluorescence presents developments in immunofluorescence as a technique for the diagnosis of virus infections. It discusses the… Read more
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Request a sales quoteRapid Virus Diagnosis: Application of Immunofluorescence presents developments in immunofluorescence as a technique for the diagnosis of virus infections. It discusses the effective and rapid methods for the diagnosis of virus infections. It addresses the application of fluorescent antibody techniques to the diagnosis and the investigation of virus infections and the assessment of their value both to the clinician and to the virologist. Some of the topics covered in the book are the fluorescence microscopy; nature of fluorescence; filter systems; transmitted light microscopy; interference filters for transmitted light microscopy; lamp centration and alignment of microscope; contrast-fluorescence condensers; photomicrography; and choice of microscope. The culture of viruses for production of antiserum is fully covered. The selection of cell lines or tissues for virus culture is discussed in detail. The text describes in depth the fluorescent antibody staining techniques. The preparation of specimens from respiratory infections is presented completely. A chapter is devoted to the respiratory syncytial virus. Another section focuses on the analysis influenza virus, paramyxoviruses, and picornaviruses. The book can provide useful information to doctors, virologists, students, and researchers.
Contents1. Introduction2. Fluorescence Microscopy Nature of Fluorescence Light Sources Filter Systems Viewing Heads Objectives Oculars Photomicrography Choice of Microscope3. The Reagents Culture of Viruses For Production of Antiserum Production of Antiserum Conjugates4. Immunofluorescence Techniques, Control of Specificity and Nonspecific Fluorescence Fluorescent Antibody Staining Techniques Control of Specificity of Fluorescent Antibody Staining Techniques Mountants Non-Specific Fluorescence Absorption Techniques5. Preparation of Specimens Preparation of Specimens From Respiratory Infections Preparation of Specimens From Infections Not Involving Respiratory Tract Preparation of Tissue Culture Cells For Fluorescent Antibody Investigations6. Respiratory Syncytial Virus7. Influenza Viruses8. Paramyxoviruses Parainfluenza Viruses Mumps Virus9. Measles (Morbilli)10. Picornaviruses11. Rabies Technical Considerations 12. Variola13. Adenoviruses Immunofluorescence and Tissue Culture Direct Examination of Specimen by Immunoflourescence For Diagnosis of Adenovirus Infection The Diagnosis of Adenovirus Infections of the Eye14. Arboviruses (Including Togaviruses, Arenaviruses, Colorado Tick Fever)15. Rubella16. Herpesvirus Hominis Investigation of Skin Lesions by Immunofluorescence Investigation of Infections of the Conjunctivae For HVH by Immunofluorescence Use of Immunofluorescence For Identifying HVH in Tissue Culture Immunofluorescence and Antiviral Treatment of HVH Eye Infections HVH and Encephalitis Disseminated HVH Infection of the Newborn Types of HVH17. Miscellaneous Viruses in the Herpesvirus Group Herpes Varicellae-Herpes Zoster Cytomegalovirus of Man Epstein-Barr Virus 18. Detection of Virus-Specific IgM by Immunofluorescence Methods For Demonstration of Virus-Specific IgM Activity Sources of Error Cytomegalovirus Rubella Herpesvirus Hominis Epstein-Barr Virus Mumps Influenza A Rotavirus Measles19. Application of Immunofluorescence Technique To Clinical Virology Management of Patients and Rational Use of Antibiotics The Use of Antiviral Drugs Virus Infection Outside Acute Stage of Illness The Use of Rapid Diagnostic Techniques in Fatal Infections Virus Infection in the Immunosuppressed Patient And Its Management Frequently Occurring Viruses Producing Unusual Symptoms Viruses Difficult To Cultivate in the Laboratory Prevention of Ward Cross-Infection With Viruses Virus Diagnosis in Areas Distant From A Virus Laboratory Rapid Diagnosis in Epidemiology Immunofluorescence As A Laboratory Aid Index
- No. of pages: 332
- Language: English
- Edition: 2
- Published: June 28, 2014
- Imprint: Butterworth-Heinemann
- eBook ISBN: 9781483192178
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