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Radiation Oncology and Radiotherapy Part C
- 1st Edition, Volume 180 - October 25, 2023
- Editors: Ai Sato, Jeffrey Kraynak, Ariel E. Marciscano, Lorenzo Galluzzi
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 9 9 0 0 7 - 3
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 9 0 0 8 - 0
Radiation Oncology and Radiotherapy Part C, Volume 180 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapte… Read more
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Request a sales quoteRadiation Oncology and Radiotherapy Part C, Volume 180 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Image-guided Radiation Therapy of tumors in preclinical models, Methods to preserve correct dosimetry in small animal irradiators, Monitoring TGFbeta signaling in irradiated tumors, Pipeline to characterize the TCR repertoire of irradiated tumors, Pipeline to identify tumor neoantigens exposed by radiation, Methods to assess abscopal responses in mice, Monitoring the biodistribution of radiolabeled therapeutics in mice, and more. Additional chapters cover Methods to assess radiation-induced fibrosis in mice, Methods to assess radiation-induced cardiotoxicity in mice, Histological assessment of intestinal injury by radiation, Methods to investigate CNS involvement in irradiated mice, Methods to characterize the exosomal output of irradiated cancer cells, and more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Cell Biology series
- Includes the latest information on the topic of development, characterization and applications in CAR T Cells
Academic, researchers, government and industrial sectors
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Radiation therapy: An old dog learning new tricks
- Acknowledgments
- Conflict of interest
- References
- Chapter 1 Image-guided radiation therapy of tumors in preclinical models
- Abstract
- 1 Introduction
- 2 Platforms for image-guided radiation therapy in preclinical models
- 3 On-board imaging for target delineation and treatment delivery
- 4 Treatment planning for subcutaneous and orthotopic tumor models
- 5 Contouring and dosimetric analysis
- 6 Treatment of subcutaneous and orthotopic tumor models
- 7 Notes
- 8 Concluding remarks
- References
- Chapter 2 Pipeline to characterize antigen-specific TCR repertoires in tumors: Examples from an HPV16 tumor model
- Abstract
- 1 Introduction
- 2 Methods
- 3 Concluding remarks
- 4 Notes
- References
- Chapter 3 Pipeline to identify neoantigens exposed by radiation
- Abstract
- 1 Introduction
- 2 Materials
- 3 Cell culture and irradiation
- 4 Genomic DNA/RNA isolation
- 5 Neoantigen identification and selection
- 6 In vitro MHC-I binding assay
- 7 In vivo immunogenicity assay
- 8 Notes
- 9 Conclusions
- Disclosures
- References
- Chapter 4 Methodologies to evaluate the radiation-induced changes on extracellular vesicles
- Abstract
- 1 Introduction
- 2 Materials
- 3 Cell culture and radiation treatment
- 4 EV isolation
- 5 Evaluation of RT-driven effects on EVs
- 6 Expression of EV markers
- 7 Cellular uptake of EVs
- 8 Conclusions
- Conflict of interest statement
- References
- Chapter 5 Monitoring TGFβ signaling in irradiated tumors
- Abstract
- 1 Introduction
- 2 Materials
- 3 Monitoring TGFβ activity ex vivo
- 4 Monitoring TGFβ in vivo using radiolabeled fresolimumab
- 5 Concluding remarks
- 6 Notes
- Acknowledgments
- Disclosures
- References
- Chapter 6 Novel platform for subcutaneous tumor irradiation in mice
- Abstract
- 1 Introduction
- 2 Material
- 3 Xenograft tumor establishment
- 4 RT treatment plan preparation
- 5 Radiation treatment procedure
- 6 Notes
- 7 Conclusion
- Bibliography
- Chapter 7 Methods to assess radiation induced abscopal responses in mice
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Generation of double tumor lesions
- 5 Monitoring tumor growth and abscopal response
- 6 Conclusion
- 7 Notes
- Acknowledgments
- Disclosures
- References
- Chapter 8 Monitoring the biodistribution of radiolabeled therapeutics in mice
- Abstract
- 1 Introduction
- 2 Instrumentation
- 3 Radiopharmaceutical administration
- 4 Dissection and sample collection
- 5 Radioassay (counting) of samples
- 6 Imaging
- 7 Analysis
- 8 Concluding remarks
- Disclosures
- References
- Chapter 9 Methods to assess radiation-induced fibrosis in mice
- Abstract
- 1 Introduction
- 2 Methods
- 3 Notes
- 4 Concluding remarks
- Acknowledgment
- Disclosures
- References
- Chapter 10 Methods to assess radiation-induced cardiotoxicity in rodent models
- Abstract
- 1 Introduction
- 2 Rodent radiation
- 3 Harvesting and processing tissues
- 4 Downstream experiments
- 5 Concluding remarks
- 6 Notes
- Acknowledgments
- References
- Chapter 11 Histological assessment of intestinal injury by ionizing radiation
- Abstract
- 1 Introduction
- 2 Radiation-induced GI syndrome in small animals
- 3 Experimental design—Rigor and reproducibility
- 4 Histological preparation of the intestines
- 5 Histological assessment
- 6 Examination of cellular dynamics of the small intestines
- 7 Non-histological assessments
- 8 Conclusions
- Acknowledgments
- Conflicts of interest
- References
- Chapter 12 A rigorous behavioral testing platform for the assessment of radiation-induced neurological outcomes
- Abstract
- 1 Introduction
- 2 Materials
- 3 Methods
- 4 Summary
- Acknowledgments
- References
- No. of pages: 312
- Language: English
- Edition: 1
- Volume: 180
- Published: October 25, 2023
- Imprint: Academic Press
- Hardback ISBN: 9780323990073
- eBook ISBN: 9780323990080
AS
Ai Sato
Ai Sato works in the University of Human Arts and Sciences in Saitama, Japan.
Affiliations and expertise
University of Human Arts and Sciences, Saitama, JapanJK
Jeffrey Kraynak
Jeffrey Kraynak is a research laboratory specialist at Stich Radiation Oncology, New York, NY, USA.
Affiliations and expertise
research laboratory specialist at Stich Radiation Oncology, New York, NY, USA.AM
Ariel E. Marciscano
Dr. Ariel E. Marciscano is an assistant professor of Radiation Oncology at Weill Cornell Medical College.
Affiliations and expertise
Assistant Professor of Radiation Oncology, Weill Cornell Medical CollegeLG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA