Pseudokinases

1st Edition, Volume 667 - May 5, 2022
Imprint: Academic Press
Editors: Natalia Jura, James Murphy
Language: English
Hardback ISBN:
9 7 8 - 0 - 3 2 3 - 9 1 5 4 1 - 0
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 9 1 5 4 2 - 7

Pseudokinases, Volume 667, the latest release in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters, inc… Read more

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Pseudokinases, Volume 667, the latest release in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters, including the Production and Purification of the PEAK pseudokinases for structural and functional studies, Structural biology and biophysical characterization of Tribbles pseudokinases, Detecting endogenous TRIB protein expression and its downstream signaling, Analysis of human Tribbles 2 pseudokinase, Expression, purification and examination of ligand-binding to IRAK pseudokinases, Characterization of pseudokinase ILK-mediated actin assembly, Biochemical examination of Titin pseudokinase, Approaches to study pseudokinase conformations, CRISPR editing cell lines for reconstitution studies of pseudokinase function, and much more.

1. Production and Purification of the PEAK pseudokinases for structural and functional studies
Isabelle S. Lucet

2. Structural biology and biophysical characterisation of Tribbles pseudokinases
Peter Mace

3. Detecting endogenous TRIB protein expression and its downstream signalling
Karen Keeshan

4. Analysis of human Tribbles 2 pseudokinase
Patrick Eyers and John Harris

5. Expression, purification and examination of ligand-binding to IRAK pseudokinases
Yogesh Kulathu and Sven Lange

6. Characterization of pseudokinase ILK-mediated actin assembly
Jun Qin

7. Biochemical examination of Titin pseudokinase
Olga Mayans

8. Approaches to study pseudokinase conformations
James Murphy, Yanxiang Meng and Cheree Fitzgibbon

9. CRISPR editing cell lines for reconstitution studies of pseudokinase function
James Murphy and Annette Jacobsen

10. Proximity ligation approaches to define the interactomes of pseudokinases
Dario R. Alessi and Toby Dite

11. Determining the role of the pseudokinase domain in guanylyl cyclase A and B signaling.
Lincoln Potter and Aaron Edmund

12. Strategies to study and stimulate bacterial pseudokinases
Seth Childers

13. Dynamics of Kinases and Pseudokinases by HDX-MS
Mark Lemmon, Joshua Sheetz and Yuko Tsutsui

14. In-cell thermal shift assay for drug binding analysis to receptor pseudokinases
Daniela Ungureanu

15. Pseudoenzymology of Kinase Suppressor of Ras
Arvin Dar

16. Computational and evolutionary insights into pseudokinase mechanisms
Natarajan Kannan

17. Methods to identify small molecule allosteric modulators of the STRAD pseudokinase
John Gordan

18. Pharmacological targeting of the pseudokinase HER3
Angus Cameron

19. Considerations and analysis of the unusual pseudokinase BUBR1
Sabine Elowe and Luciano Gama Braga

20. The pseudokinase domain of receptor guanylyl cyclases: binding of ATP and allosteric regulation of guanylyl cyclase activity
Sandhya Visweswariah

21. Methods for discovering catalytic activities for pseudokinases
Vincent Tagliabracci and Krzysztof Pawlowski

22. Efficient Expression, Purification, and Visualization by Cryo-EM of Fully-Glycosylated Unliganded HER3
Natalia Jura and Kliment Verba

23. An effective strategy for ligand-mediated pulldown of the HER2/HER3 receptor complex and cryo-EM structure determination at low sample concentrations
Natalia Jura

24. Identification of small molecule binders for pseudokinases
Stefan Knapp and Sebastian Mathea

25. Identification and characterization of Tyk2-JH2 pseudokinase domain inhibitors
Gregory Locke and Christopher Barbieri

26. Monitoring the ATPase activity of Bud32 and its role in tRNA binding
Jonah Beenstock and Frank Sicheri

Natalia Jura

Dr. Natalia Jura is an Associate Professor and an Investigator in the Department of Cellular and Molecular Pharmacology at the Cardiovascular Research Institute within the School of Medicine at the University of California, San Francisco (UCSF). She is also an Associate Director of the Quantitative Biosciences Institute at UCSF. Dr. Jura's group at UCSF focuses on understanding how soluble protein kinases and membraneassociated receptor kinases assemble into functional complexes and regulate their signaling through molecular interactions with regulatory proteins. Her group also investigates alternative non-catalytic roles of protein kinases as scaffolds in cellular signaling pathways. They apply this knowledge for design of small molecule inhibitors that target these poorly understood kinase functions in human diseases. Dr. Jura received her M.S in biochemistry from Jagiellonian University in Krakow, Poland and her Ph.D. in molecular and cellular biology from Stony Brook University.

Affiliations and expertise

Assistant Professor, Cardiovascular Research Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, USA

James Murphy

James Murphy is Associate Professor and the head of the Inflammation Division at the WEHI (formerly known as The Walter and Eliza Hall Institute of Medical Research) in Melbourne and is closely associated with The University of Melbourne and The Royal Melbourne Hospital. James’ lab studies the protein-protein interactions that underpin signal transduction. Much of his work is focused on understanding the molecular mechanisms by which protein kinases and their relatives, pseudokinases, regulate cell signaling. Their work on the pseudokinase, MLKL, provided a template for developing a detailed understanding of how the remaining ~50 uncharacterized pseudokinases modulate cell signaling.

Affiliations and expertise

Associate Professor, Head, Inflammation Division, Walter and Eliza Hall Institute of Medical Research, Victoria, Australia

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