Protides of the Biological Fluids
Proceedings of the Thirty-Third Colloquium, 1985
- 1st Edition, Volume 33 - January 1, 1985
- Imprint: Pergamon
- Editor: H. Peeters
- Language: English
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 7 9 7 6 - 3
An International Review Series Devoted to Proteins and Related Studies, Volume 33: Protides of the Biological Fluids documents the proceedings of the 33rd Colloquium held in 1985.… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quoteAn International Review Series Devoted to Proteins and Related Studies, Volume 33: Protides of the Biological Fluids documents the proceedings of the 33rd Colloquium held in 1985.
This book is divided into three sections. Section A focuses on the plasma protein genes that includes the location of genes on the chromosome, pathology and variance of several genes, and gene expression. The proteins of the nucleus and detection of antibodies, such as antinuclear antibodies (ANA), to nuclear constituents are deliberated in Section B. Section C is devoted to the technical advances in the field of protein chemistry.
This book is a good reference for students, researchers, and medical practitioners conducting work on the protides of biological fluids.
Preface
Acknowledgments
The Fourteenth Arne Tiselius Memorial Lecture
Progress in Plasma Proteins: Structure, Function, and Genes
Section A. Plasma Protein Genes
A.1. Gene Structure
Organization and Chromosomal Locations of Genes Encoding Human Plasma Proteins
Sequence Analysis of Human Immunoglobulin Heavy-Chain Genes
Structure and Evolution of the Serum Albumin Gene Family
Molecular Genetics of Human Transferrin
Apolipoprotein A-II Gene Structure: Evolutionary Implications
Molecular Genetics of Haptoglobin
Human Complement Genes of the Major Histocompatibility Complex
Coagulation and Fibrinolysis: Characterization of the Human Prothrombin and Human Tissue-Plasminogen Activator Genes
The Presence of Multiple Polyadenylation Sites in the Gene for the β and γ Chains of Human Fibrinogen
The Human Factor VIII Protein
Structure of the Gene for Human Factor IX
Structure and Evolution of the Human Antithrombin III Gene
The Structure of the Human Plasminogen Activator (uPA) Gene
A.2. Chromosomal Location
Chromosome Mapping of Serum Protein Genes
Cloning and Expression in Micro-Organisms of the Genetic Information Coding for Human Plasma Proteins
In Situ Hybridization for Chromosomal Mapping of Plasma Proteins
In Situ Hybridization: Location of Plasma Protein Transcription
Structure and Liver Specific Expression of the Human Retinol Binding Protein Gene
Organization of the Genes Coding for Prothrombin and Factor X
Mapping Tissue Plasminogen Activator and Urokinase Genes to Human Chromosomes
Molecular Genetics of Human Antithrombin III Deficiencies
Genetically Abnormal Human Fibrinogens — A Summary of 32 Structurally Elucidated Variants
Linkage of Human Apolipoprotein AI, CIII and AIV Genes
Genetic Polymorphisms and Linkage Relations of Complement Factor C8
Close Genetic Linkage Between Four Plasma α-Protease Inhibitor Loci (Pi1, Po1A, Po1B, Pi2) in Pigs
Genomic Evidence for the Truly Allelic Nature of the B-Locus Polymorphism of the Rabbit Immunoglobulin Light Chain
A.3. Cloning and Expression
Genetic Studies on a Human Plasma Transthyretin (Prealbumin) Variant Associated with Familial Amyloidotic Polyneuropathy
Gene Organization and DNA Polymorphism of the Human Transferrin Gene
Expression in E. coli of Human Preprourokinase cDNA
Cell Cycle Dependent Variations in the Distribution of the Nuclear Protein Cyclin (PCNA) in Cultured Cells
Organization and Evolution of the Mouse Complement C4 Isotypes
Altered Inhibition Properties of α1-Antitrypsin Variants Constructed by Site-Directed Mutagenesis
Structure of the Human Haptoglobin Pseudogene
Molecular Cloning, Sequencing and Expression of a cDNA Coding for Human Antithrombin III
Purification of Active Human α1-Antitrypsin From Genetically Engineered Micro-Organisms
Regulation of Rat α2-Macroglobulin Gene Activity
Influence of Neonatal Androgenization on the Expression of α2u-Globulin Genes
Preferential Segregation of Murine Chromosome 11 in TCGF-Independent Rat x Mouse T-Cell Hybrids
Individual Polymorphism of Albumin Gene Studied by Sequencing DNA
Section B. Antinuclear Antibodies (ANA)
B.1. Chemistry of Nuclear Antigens
Fine Structure of snRNPs
Anti-Sm and Anti-La Murine Monoclonal Antibodies: Characterisation of the Antigens
Antihistone Antibodies Bind to Restricted Epitopes
IP-Heterogeneity of Nuclear Antigens Detected in an Isoelectric Focusing-Immunoblot System
Characterization of Nuclear and Cytoplasmic Autoimmune Antigens
Analysis of Nuclear and Cytoplasmic Protein Antigens in Systemic Rheumatic Disease
Interaction of VA RNA with the LA Antigen of HeLa Cells and Frog Oocytes
Characterization of an Ubiquitous S-Phase Related and Chromatin Associated Antigen with Human Autoantibodies
A 80 KD Protein is Associated with the Nucleolus Organizer (NOR) of Human Cell Lines
Polymorphism of Epstein-Barr Virus Nuclear Antigen (EBNA)
Immunochemical and Biological Properties of Anti-Nucleic Acid Antibodies in SLE
Recognition of dsDNA by Anti-DNA Antibodies. Immune Complex Formation and Further Potential Biological Consequences
Crossreactivity of Monoclonal Antibodies to DNA: Related to Avidity?
Diversity and Multispecificity of Autoantibodies Reactive with DNA: Some Evolutionary Implications
Isolation and Characterization of HP10: A Specific Human Hepatic Protein cDNA
ANA With Dual Specificity for the Histone Octamer and a Plasma Membrane Antigen React with H2B in Immunoblot but not in Immunosedimentation
Biochemical Mechanism of Chromosome Banding Analysed by Monoclonal Anti-DNA Antibody
Immune Response of Genetically Normal Mice Towards Isolated Native U1 Small Nuclear Ribonucleoproteins: Evidence that U snRNPs may Function as an Immunogen During the Anti-RNP/Sm Autoantibody Response
Auto-Antigens of Nuclear Ribonucleoprotein Particles
The Antigenic Structure of Histones
Immunochemical Study of Post Synthetic Histone Modifications on Chromatin Conformation
Immunohistochemical Study During Phylogenesis of a Nervous Tissue Subnuclear Protein Fraction
Monoclonial Antibodies and Human Autoantisera Used for the Detection and Identification of Nuclear Matrix Compounds
Extracellular Release of Nuclear SSB-Antigen by Adenovirus Infection and Interaction with Anti-SSB Antibody
Autoantibodies to Highly Purified Nuclear Proteins in Aging
B.2. Autoantibodies in Disease
B.2.1. SLE
Autoantibody-Profiles in Patients with SLE, Scleroderma and Overlap-Syndromes
Anti-ENA Antibodies in Systemic Lupus Erythematosus: Clinical-Serological Correlations
The Qualitative and Quantitative Aspects of Anti-dsDNA Antibodies in Systemic Lupus Erythematosus
Role of Sex Steroids in Systemic Lupus Erythematosus (SLE)
Cross-Reactivity of Anti-DNA Antibodies: A New Clue for the Pathogenesis of SLE?
Anti-ENA Antibodies in Systemic Lupus Erythematosus: A Follow-Up Study
Complement, IgA, CNS and Mortality in SLE
Clinical Differences Between S.L.E. Patients in Relation to the Avidity of their Anti-dsDNA
Antibodies to the Histones: Different Patterns of Response in Idiopathic SLE and Drug-Induced Lupus
Antibodies Against Phospholipids (Cardiolipin) in SLE
Clonal Analysis of Autoantibodies in Autoimmune Thyroiditis and SLE
Multiple Ligand Binding by Sera From Systemic Lupus Erythematosus Patients
B.2.2. Other Diseases
The Immunopathogenesis of Primary Sjögren's Syndrome
Autoantibodies in Sjögren's Syndrome: Comparison of Autoantibody Determination Methods Show that Antinuclear Antibody and Rheumatoid Factor are Associated with Ro/SSA Precipitin Formation
Autoantibodies to Transfer RNA-Related Antigens in Myositis
Diversity of Antinuclear Antibodies in 42 Cases in Polydermatomyositis (PM/DM)
Are Anti-nRNP Levels Specific Guides for Assessing Disease Activity in Patients with (Mixed) Connective Tissue Disease?
Diagnostic and Prognostic Significance of Anti-Centromere Antibodies (ACA)
Nuclear Antibodies in Autoimmune Liver Diseases Defined by Immunoprecipitation and Cell Culture Substrates
Antiphospholipid Antibodies in Chronic Active Hepatitis: Lack of Correlation with Anti-DNA Antibodies
Soluble Immune Complexes (SIC), Rheumatoid Factor (RF) and Auto-Antibodies in Leprosy Patients' Sera
Antigenic Heterogeneity as Reflected by Interaction with Antibody (Ab) and Circulating Immune Complexes (CIC) in Sarcoma Patients
B.3. ANA Determination
B.3.1. Detection of Autoantibodies
The Detection of Autoantibodies: An Overview
Ultrastructure of HEp-2 Cells
Use of HEp-2 Cells in the IF Test for Detection of Antinuclear Antibodies
Correlation of Albumin mRNA Levels with Secretion Rates in Fetal Rat Hepatocyte Cultures Exposed to Dexamethasone
What is the Primary Target of Anti-DNA Auto-Antibodies?
B.3.2. Assays
Quantitation of Antibodies to Nucleoribonucleoprotein (nRNP) by ELISA: Relation Between Antibody Levels and Disease Activity in Patients with Connective Tissue Disease
Immunoblotting as a Diagnostic Tool in Connective Tissue Diseases
Immunoblotting with Nuclear Antigens: A Study of the Diagnostic Value
ELISA for Antibodies to Ro-Antigen. Diagnostic Value, Compared to Antibodies to dsDNA, Histones, RNP, Sm- and SSB-Antigen
Affinity Purification and Immunoassay of Anti-Ro/SSA
A Practical Quantitative Method for Specific Antinuclear Antibodies
Application of the Dot Immuno-Binding Assay to Connective Tissue Disease
A Comparison of Three Methods for the Assay of Anti-DNA Antibodies
Enzyme Immunoassay for the Detection of Autoantibodies Against the Nuclear Protein Scl-70
Single Radial Immune Haemolysis (SRIH) Assay for the Measurement of Complement Fixing Antibodies
Section C. Methodologies
C.1. Isoelectric Focusing (IEF)
C.1.1. Unidimensional (1D)
Electrofocusing in Wide pH Range Immobiline Gels Containing Carrier Ampholytes
Patient-Specific Banding Pattern in Isoelectric Focusing-Immunoblot with Nuclear Antigens
Evaluation of Ready-Made IEF Gels for the Demonstration of IgG Oligoclonal Bands in Cerebrospinal Fluid
IEF of Albumin in Low Denaturing Media. I: Analytical Aspects Related to Uremia
IEF of Albumin in Low Denaturing Media. II: Preparative Aspects
pH Gradient Manipulation within the pH 4-6 Range Using Separator Isoelectric Focusing
C.1.2. Two Dimensional (2D)
Immobilized pH Gradients as First Dimension for 2D Separations
IEF and 2D-Electrophoresis with Narrow and Ultranarrow Immobilized pH Gradients for the Analysis of Genetic Variants
Genetic Studies of Apolipoprotein A-IV by Two-Dimensional Electrophoresis
Two-Dimensional Polyacrylamide Gel Electrophoresis of Escherichia coli Proteins with Pharmalytes
Two-Dimensional Electrophoretic Analysis of Genetic Muscle Disorders
Quantitative Immunoelectrophoretical Determination of Some "Intrathecal Synthesized" Proteins
Examinations of Monoclonal IgG with Preparative Chromatofocusing Followed by 2-Dimensional Gel Electrophoresis
Two Dimensional Immunoblot Electrophoresis for Characterization of Pancreas Specific Oncofoetal Antigens
Applications of Small-Column Chromatofocusing and 2-Dimensional Gel Electrophoresis in CSF and Serum Protein Examinations
C.2. Isotachophoresis
Isotachophoretic Analyses of Compounds in Complex Matrices: Allergenic Extracts and Aluminium in Biological Fluids and Bone
C.3. Gel Electrophoresis
Micro Gel Electrophoresis
Limits and Feasibilities of Gel Densitometry
Sieving of Rod-Shaped Viruses During Electrophoresis in Agarose Gels
Electrophoretical Separation of Urinary Alanine Aminopeptidase (=AAP) Isoenzymes in Ultrathin Agarose Gels
Horizontal Ultrathinlayer SDS Poregradientgel Electrophoresis for the Differentiation of Proteinurias
Apparatus for Fast Electroelution of Microgram Quantities of Proteins from Polyacrylamide Gels
Non-Invasive Diagnosis of Kidney Diseases Applying Sensitive Thin-Layer SDS-PAA Gradient Gel-Electrophoresis
C.4. High Performance Electrophoresis (HPE)
Analytical and Microprepartive High-Performance Electrophoresis
C.5. High Performance Liquid Chromatography (HPLC)
An Automated Tandem HPLC System Applied to Peptide Mapping of Human Plasma Proteins
Isolation and Characterisation of Fish Protamines by Reversed-Phase High-Performance Liquid Chromatography
Usefulness of a Multicolum FPLC-System for Fractionation and Monitoring of Proteins and Peptides in Uremie Serum, Hemofiltrates an Dialysates
Broad pH-Gradient Chromatofocusing
Studies on the Analysis of Brain Structural Proteins by Chromatofocusing
Size Exclusion Chromatography of Proteins on Improved Bonded Silica Columns
An Optimized Method to Measure Glycosylproteins: HPLC Assay of 5-Hydroxymethyl-2-Furfuraldehyde (5-HMF)
Reversed Phase High Performance Liquid Chromatography in Studies of the Substance P Turnover
C.6. Immunoblotting
Screening for Monoclonal Antibodies Against a Mycoplasmal Membrane Protein by Protein Electroblotting
An Economical Design of Immunoblotting Tank
Immunoblotting of Human Pepsinogens
C.7. Staining Methods
Quantitative Aspects of Protein Staining by Coomassie Blue Dyes and Silver Staining
Sensitive Protein Stain on Nitrocellulose Blots
Studies of Antithrombin III Charge Heterogeneity
Silver Staining and SDS Polyaerylamide Gel Electrophoresis of Antibacterial Proteins in Bovine and Human Milk
Immunogold Staining of Blotted Proteins — Demonstration that Fibronectin Initiates the Immunoglobulin Cryoprecipitation
Statistical Analysis of Interference Between α, β, γ Globulines and Serum Albumin Determination with Dye Binding by Bromcresol Green and Bromcresol Purple
C.8. Antibodies and Monoclonals
A Practical Method for Light Chain Quantification for Each Immunoglobulin Class
Isolation, Characterization and Quantitation of Murine IgE
Production of Monoclonal Antibodies to Dog Post-Gamma Globulin
Interaction of Human Chorionic Somatomammotropin Monoclonal Antibodies with Fragments Derived from the Nicked Form of the Hormone
Purification of a Single-Chain Urokinase Precursor from A413 Human Epidermoid Carcinoma Cells by Monoclonal Antibody Immunoaffinity Chromatography
A Simple Method for Isolating Pig Zona Pellucida and the Detection of Anti-Zona Antibody in Relation to Infertility by Immunofluorescence and Immunoenzymatic Methods
Anti-Thymocyte Antibodies in Myasthenia Gravis: Their Relation to Anti-AchR Antibodies
Analysis by Monoclonal Antibodies of Cell Surface Structures Marking Discrete Steps of Cell Differentiation
A Triple Immunoenzymatic Method for Histo-Pathological Studies: Detection of IgG, IgM and Ig A Positive Cells Simultaneously in Single Tissue Section
C.9. Other Methodological Papers
Bioreactors with Laminar and Defined Gradient Hydrodynamics: Hydraulic Culture of Cells for Assessment of Effectors Controlling Regenerative Tissue Morphogenesis in Wound Healing
Separation of Nonenzymatically Glycosylated Proteins by Phenylboronate Affinity Chromatography
Glycosylated LDL Analyzed by Electrophoretic Mobility (E.M.)
Comparison of an ELISA and a Radial Immuno Diffusion Method for the Determination of Factor VIIIR : Ag
Cellular Adhesion to Low Molecular Weight Connective Tissue Matrix Proteins
Tissue Plasminogen Activator in the Cytosol of Breast Tumors: Technical Aspects and Correlations with Commonly used Prognostic Factors
Use of Immunoprecipitates to Produce Anti-BCG Cell Wall Antibody of Restricted Specificity from Mycobacterium BCG
An Updated Method for the Purification of Mycobacterium leprae and Other Mycobacteria from Infected Tissues
Lack of Receptors for Fibronectin on Leukemic Granulocytes
Detection and Immunohistochemical Localisation of hPLAP and CA 125 in Sera and Tissues of Patients with Ovarian, Nonovarian Tumors, and Liver Disorders
Author Index
Subject Index
- Edition: 1
- Volume: 33
- Published: January 1, 1985
- No. of pages (eBook): 722
- Imprint: Pergamon
- Language: English
- eBook ISBN: 9781483279763
Read Protides of the Biological Fluids on ScienceDirect