Protides of the Biological Fluids

Preface

Acknowledgments

Academic Lecture

Science and Society

The First Arne Tiselius Memorial Lecture

Separation Methods: Tools of Progress in Proteins

Section A: Monoclonal Immunoglobulins (MIg)

A1. Properties and Structure of MIg

Principles of Immunoglobulin Structure in Relation to Aberrations in Protein Metabolism

Monoclonal IgM-Globulin with Anti-IgG Activity: An Immunofluorescent and Electron Microscopic Study

Structural Studies of Human IgG Monoclonal Proteins Using Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis

Insolubilization of a Fraction of Human IgG in a Salted Phenol Solution

A2. The Amyloid Substance (AS)

A2.1 Chemical, Immunochemical and Ultrastructural Studies of the AS

The Immunoglobulin Origin of Amyloid Fibril Proteins

The Subunit Structure and Immunologic Properties of Amyloid Fibrils

Ultrastructure and Composition of Amyloid — Variable or Constant

The Chemical Classes of Amyloid Substance in Men, Monkeys and Ducks

Isolation and Characterization of a Low Molecular Weight Peptide from Human Amyloid

A Non-immunoglobulin Protein — The Major Component of Some Human Amyloids

Studies on the Antigenic and Immunochemical Properties of Amyloid Fibrils

Studies with Conformational Antisera to Amyloid

The Acid-soluble Fraction of Amyloid — The Protein Subunit of the Amyloid Fibril

Similarity in Amino Acid Composition between Human Light Chain and Some Amyloid Proteins

A2.2 Production and Deposition of the AS in Cells and Tissues

Vital Dyeing Processes and Exchange Phenomena at the Amyloid Deposits

Interactions of Congo Red and Toluidine Blue with Amyloid

Cellular Immunity and Amyloidosis

A2.3 Clinical and Experimental Aspects of Amyloidosis

Experimental Amyloidosis and High Dosage Tolerance to Casein

F1, Rauscher and Moloney Leukemia Viruses Inhibiting and Enhancing Amyloid Development in Casein-treated C3H Mice

Amyloidosis, Multiple Myeloma and Aging

Coincidence of Amyloidosis and Monoclonal Gammopathies — A Study of 82 Cases

Senile Amyloidosis of Arterial Elastica Interna

Pathogenic Mechanisms in Experimental Murine Amyloidosis

Amyloidosis and Immunology. Amyloid Degeneration and Lingering Tuberculosis in the Aged

A3. The Antibody Function of MIg

A3.1 Restricted Antibodies

Monoclonal Antibodies to Streptococcal Polysaccharides in Rabbit and Mouse

Limited Heterogeneity of Antibodies in Individual Animals

Partial Amino Acid Sequence of Rabbit Antibody Heavy Chain Variable Regions

Deviations in the Balance of Intracellular Heavy- and Light-Chain Determinants in Human Plasma Cells

Genetic Determination of Idiotypes in Antibodies to the Group-specific Polysaccharides of Streptococci

The Number of Unique Monoclonal Antibodies Against a Simple Hapten in CBA Mice

Mouse Antibodies with Electrophoretic Restriction

Kappa-type Immunoglobulin Light Chains of Rat Strain LOU/Wsl

A3.2 Pathological MIg

An IgM Waldenstrom Protein with Anti-phosphorylcholine Specificity

Extreme ASO Activity in an IgG Kappa Myeloma Protein

Monoclonal Immunoglobulins at the Lymphocyte Surface in Immunoproliferative Disorders

Immunoglobulin Analysis in Families of Myeloma Patients

Lymphocytotoxic Activities of Sera from Myeloma and Other Paraproteinemic Patients

Paraprotein Production and Induction of Glomerulonephritis by Mouse Plasmacytoma Cells Transferred to Hamsters

Complement Levels in Patients with IgG Monoclonal Gammopathies

Correlations Between the Serum Concentrations of IgG Myeloma Proteins and Background IgG

Clinical Significance of Bence-Jones Proteinuria

Immunoglobulin Restricted Heterogeneity in Subacute Sclerosing Panencephalitis

Paraproteinaemia in Blood Donors and the Aged: Benign and Malignant

Transitory Appearance of Homogeneous Immunoglobulins — "Paraproteins" — in Children with Severe Combined Immunodeficiency Before and After Transplantation Treatment

The Stability of Serum Immunoglobulin Levels in Health and Rheumatoid Arthritis

The IgG Subclass Distribution in Double M-component Sera

J-chain of Polymeric IgA Myeloma Proteins

Section B: Active Polypeptides derived from Plasma Proteins

B1. Fibrinopeptides

Solid Phase Synthesis of Human Fibrinopeptides A and B

A Quantitative Study of Physiologic Effects of Peptides Derived from Human Fibrinogen by Plasmin Digestion

The Effect of Fibrinogenolytic Products on Plasma Fibrinogen Concentration

Preparation and Clinical Use of Antibody Specific to Fibrinopeptide A

Prevention of Fibrinogen Coagulation by Specific Antifibrinogen Antibodies

B2. Kinin System

The Interrelationship Between the Clotting, Fibrinolytic and Kinin Systems of Human Plasma

Application of an Insoluble Hageman Factor Complex to Kinin Research

Inhibitors of the Pulmonary Destruction of Bradykinin in the Rat

On the Inhibitory Capacity of Human Plasma Protein Fractions Against Plasminogen Activation

Some Physico-chemical Parameters of the Perivascular Plasminogen Activator Angiokinase

Kallikreinogen and Kallikrein Inhibitor in Cerebral Stroke

The Role of Bradykinin in the Inflammation of Skin

Kininase Activity of the Blood in Various Haematological States

The Effect of Urea on the Release from Proteinic Macromolecules of Some Factors with Kininic and Stimulator Activity of Phagocytosis

Evidence for Kinins, Histamine and Complement as Mediators of the Inflammatory Response

B3. Angiotensin and Renin System

The Metabolism of Angiotensin I by Endothelial Cells

Angiotensin Receptors in Smooth Muscle Cell Membranes

Renin in the Spontaneously Hypertensive Rat

B4. Anaphylatoxins

Anaphylatoxin and Other Biologically Active Products of Complement

Enzymatic Formation of Active Polypeptides in the Human Skin

Generation of Anaphylatoxin Activity Through Bypass Activation of the Complement System

Regulation of Chemotaxis of Leucocytes by the Anaphylatoxin-related Peptide System

B5. Protease Inhibitors

New Proteinase Inhibitors in the Secretion of Some Mucous Membranes of the Human Body

Protein Proteinase Inhibitors in Male Sex Glands and their Secretions

Fibrin-agar-electrophoretic Study of Polyvalent Proteinase Inhibitors from Snails

Low-molecular-weight Proteinase Isoinhibitors of Broad Specificity from the Skin of Snails

Preparation of Human C1 Esterase Inhibitor Concentrate and its Possible Clinical Use

Section C: Microcalorimetry in Biological Systems

C1. Thermodynamics: Structure and Interaction Studies

Calorimetric Studies of Hapten-Antibody Interaction

Calorimetric Studies of Binding of Substrates and Inhibitors to Triosephosphate Isomerase

Thermodynamics of Interaction of Pyridoxal Phosphate with Transaminase and Model Systems

Analytical and Microcalorimetric Investigation of the Adsorption of Human Serum Albumin on Well-defined Polystyrene Latices

Study of Competition Binding on BSA by Microcalorimetry

Calorimetric Study of Bound Water in Protein Solutions and in Human Red Blood Cells

Microcalorimetric Investigations of Biopolymer Aqueous Solutions

Thermodynamic Studies on Iron (III) Exchange Between Chelates and Human Transferrin

Conformational Study of Lysozyme in the Crystal and in Solution, Using Microcalorimetry

A Microcalorimetric Study of Haptoglobin — Hemoglobin Interaction

Investigation of Carrier Antibiotics by Computerized Microcalorimetry

Thermodynamics of Protein Interactions

A Calorimetric Study of the Intluence of Ionic Strength on the Binding of Inhibitors to Ribonuclease A

Calorimetric Investigations of the Interaction of Inhibitors and Substrates with Ribonuclease A and Ribonuclease T1

C2. Applications to Biological Systems

Use of Flow Microcalorimetry for Activity Determination of Proteolytic Enzymes

Investigation of Time-dependent Processes by Heat Flow Calorimetry

Microcalorimetric Experiments on a Cell-free Protein Synthesizing System of Saccharomyces

The Action of Thrombin on Platelets: A Microcalorimetric Study

Metabolic Activity of Erythrocytes as Determined by Microcalorimetry

The Measurement of Kinetic and Thermodynamic Parameters for Reacting Systems by Flow Microcalorimetry and Application of the Method to the Urea—Urease System in a Sodium Phosphate Buffer

Matrix-bound Enzymes Studied by Flow Microcalorimetry

Section D: Other Techniques

The Double-line Method; Simultaneous Determination of Both Light-chain Types in Monoclonal and Polyclonal IgG

Radioimmunoassay of α-Fetoprotein

Quantitative Study of Tests Using Latex Particles Coated with Proteins or Peptides

Detection of Albumin- IgG Complexes in γ-globulin Preparations

The IgM Latex Test, a Simple Method for Rapid Detection of Infections in the Neonatal Period

Author Index

Subject Index