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Probes and Membrane Function
1st Edition - January 1, 1971
Editors: Britton Chance, Chuan-pu Lee, J. Kent Blasie
eBook ISBN:9781483271484
9 7 8 - 1 - 4 8 3 2 - 7 1 4 8 - 4
Probes of Structure and Function of Macromolecules and Membranes, Volume I: Probes and Membrane Function discusses the developments in techniques for the measurement of rapid… Read more
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Probes of Structure and Function of Macromolecules and Membranes, Volume I: Probes and Membrane Function discusses the developments in techniques for the measurement of rapid reactions and detection of enzymatic reactions in simple and complex systems. This book describes the application of the incisive techniques employed for the study of activity-related structural changes in enzymes to functionally related structural changes in membranes. The structural changes initiated in response to energy coupling and ion movements in mitochondria and axons are also elaborated. This publication covers the basic mechanisms by which structural changes can participate in energy coupling at the redox level of the hemoproteins that leads to the evaluation of the function of ubiquinone and cytochrome b in energy coupling. The function of factors that couple electron transport and energy conservation is likewise considered. This volume is a good reference for students and researchers conducting work on the structure and function of macromolecules and membranes.
Contributors
Preface
Contents of Volume II
Introductory Remarks
General Introduction to Responsive Probes
Part 1. Interpretation of Data from Probe Studies
Introduction to Fluorescence Probes
The Effects of Chemical Environment on Fluorescence Probes
Discussion
The Use of Specific Chromophoric Inhibitors in Investigations of Biological Reactions in Solution, in Crystals, and in Membranes
Structural Sensitive Aspects of the Electronic Spectrum
Discussion
Introduction to Magnetic Resonance Techniques
The Nuclear Magnetic Resonance Spectroscopy of Proteins
Paramagnetic Probes in Enzyme Systems
Enzyme-Ligand Interactions as Detected by Nuclear Relaxation Rates of Substrates and Inhibitors
Electron-Nuclear Double Resonance (ENDOR) from Randomly Oriented Biomolecules
Electron Paramagnetic Resonance of Single Crystals as Environmental Probes
Discussion
Optical Activity and Conformation of Proteins
Near Infrared Circular Dichroism: d → d Transitions in Hemoproteins
Discussion
Introduction to X-Ray Diffraction and Electron Microscopy Studies
Protein Conformational Changes Studied by Diffraction Techniques
Low-Angle X-Ray Diffraction of Biological Membranes
Probing Membrane Structure with the Electron Microscope
Characterization of a Hydrocarbon Soluble Protein/Lipid Complex
Discussion
Part 2. Structural Control of Reactivity of Membranes
State Changes of the Mitochondrial Membrane as Detected by the Fluorescence Probe 1-Anilino-8-naphthalene Sulfonic Acid
Probes of Macromolecular and Molecular Structure in the Membranes of Mitochondria and Submitochondrial Vesiqles
Fluorochrome Labeling of Chromatophores of Photosynthetic Bacteria with Auramine O
Fluorescence Changes in Dye-Treated Nerve following Electric Stimulation
Anilino-1, 8-naphthalene Sulfonate and Brom thymol Blue Responses to Membrane Energization
Kinetic Analysis of MNS Interaction with Submitochondrial Particles
Discussion
Spin Labeling of Submitochondrial Membrane
Discussion
Response of ANS to Ca++ Binding in Mitochondrial Membrane as Affected by Local Anesthetics
K+ Gradients in Submitochondrial Particles
K+ Transport in Submitochondrial Particles
Glutaraldehyde as a Probe of Metabolism-Linked Changes in the Mitochondrial Proteins
Calcium Transport in Sonicated Submitochondrial Particles
General Discussion: Responses of Probes to Ionic Gradients
Use of 8-Anilino-1-naphthalene Sulfonate as Conformational Probe on Biological Membranes
ANS Fluorescence as an Indicator of Ionic Interaction with Membranes
Interaction of Fluorescent Probes with Hemoglobin Free Erythrocyte Membranes
The Design of Fluorescent Probes for Membranes
Conformational Activity vs. Ion-Induced Osmotic Perturbation in the Transformation of Mitochondrial Ultrastructure
Is There a Mechanochemical Coupling in Mitochondrial Oxidative-Phosphorylation?
X-Ray Diffraction Analysis of Myelin
General Discussion: The Role of Structure in the Response and Location of Probes in Natural and Artificial Membrane Systems
The Problem of Cytochrome b
Approaches to the Relation of Structure and Function of the Inner Mitochondrial Membrane
Interactions of Ubiquinone and Cytochrome b in the Respiratory Chain
Plasticity in the Responses of Ubiquinone and Cytochrome b
Discussion
Localization of Succinate Dehydrogenase in the Mitochondrial Inner Membrane
The Two Sides of the Inner Mitochondrial Membrane
Biosynthetic Origin of Cytochrome Oxidase
Localization of Cytochrome c and Cytochrome Oxidase in the Mitochondrial Inner Membrane
On Branching among Mitochondrial Respiratory Chains
Where Is the Mitochondrial Binding Site for Cytochrome c?
The Presence of Denatured Mitochondrial Adenosine Triphosphatase in "Structural Protein" from Beef-Heart Mitochondria
Reconstitution of Highly Resolved Particles with Succinate Dehydrogenase and Coupling Factors
Contaminants in "Purified" Energy Transfer Factors of Oxidative Phosphorylation
Computer Analysis of Electron Transport Kinetics
Discussion: Ordering of Membrane Components: The Sidedness Problem
General Discussion on Part 2
Part 3. Instrumentation Developments
Computer Processing and Analysis of EPR Spectra
Rotating Drum Densitometry Techniques
The Electric Field Jump Relaxation Method
Chemical Applications of Light Scattering: Scattering of Coherent Light and Chemical Fluctuations