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Elsevier

Plant Tissue Culture

Techniques and Experiments

  • 4th Edition - February 17, 2021
  • Latest edition
  • Author: Sunghun Park
  • Language: English

Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant cultur… Read more

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Description

Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists.

Key features

  • Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement
  • Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement
  • Contains the latest advances and updates since the previous edition published in 2012

Readership

Undergraduate and postgraduate students and researchers focusing on plant tissue culture/micro-propagation, horticulture, botany, biotechnology and plant physiology

Table of contents

1. History of plant tissue culture

2. Setup of a tissue culture laboratory

3. Media components and preparation

4. Explant preparation

5. Contamination

6. Callus induction

7. Regeneration and morphogenesis

8. Woody shrubs and trees

9. Haploid plants from another culture

10. Embryo rescue

11. Meristem culture for virus-free plants

12. In Vitro propagation for commercial production of ornamentals

13. Protoplast isolation and fusion

14. Agrobacterium-mediated transformation of plants

14-1. Petunia or tobacco leaf disk

14-2. Petunia shoot apex

14-3. Tobacco leaf infiltration

14-4. Arabidopsis floral dip transformation

14-5. Dicot plant-based techniques and experiments

14-6. Rice transformation techniques and experiments

15. Genome edition and RNAi

15-1. Tomato genome edition

15.2. Lettuce RNAi

Product details

  • Edition: 4
  • Latest edition
  • Published: February 17, 2021
  • Language: English

About the author

SP

Sunghun Park

Sunghun Park is Professor at the Department of Horticulture and Natural Resources at Kansas State University, USA. He earned his PhD in Plant Physiology from Texas A&M University in 1995 and his main research areas include abiotic stress physiology, crop improvement using plant tissue culture and genome editing technology.
Affiliations and expertise
Professor, Department of Horticulture and Natural Resources, Kansas State University, Manhattan, KS, USA

View book on ScienceDirect

Read Plant Tissue Culture on ScienceDirect