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Plant Tissue Culture
Techniques and Experiments
- 4th Edition - February 17, 2021
- Author: Sunghun Park
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 8 2 1 1 2 0 - 5
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 8 5 1 3 7 - 4
Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant cultur… Read more
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Request a sales quotePlant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists.
- Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement
- Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement
- Contains the latest advances and updates since the previous edition published in 2012
Undergraduate and postgraduate students and researchers focusing on plant tissue culture/micro-propagation, horticulture, botany, biotechnology and plant physiology
- Cover image
- Title page
- Table of Contents
- Copyright
- Preface
- Acknowledgments
- Scheduling and interrelationships of exercises
- Chapter 1. History of plant cell culture
- Abstract
- Chapter Outline
- Introduction
- The early years
- The era of techniques development
- The recent past
- The present era
- References
- Chapter 2. Setup of a tissue culture laboratory
- Abstract
- Chapter Outline
- Work areas
- Reference
- Further reading
- Chapter 3. Media components and preparation
- Abstract
- Chapter Outline
- Inorganic salts
- Plant growth regulators
- Vitamins
- Carbohydrates
- Hexitols
- Gelling agent
- Amino acids
- Antibiotics
- Natural complexes
- Media pH
- Medium preparation
- References
- Chapter 4. Explant preparation
- Abstract
- Chapter Outline
- Explant age
- Season
- Explant size
- Plant quality
- Goal
- Genotype
- Aseptic technique
- Aseptic germination of seeds
- Reference
- Further reading
- Chapter 5. Contamination
- Abstract
- Chapter Outline
- Explant
- Personnel
- Laminar airflow hood
- Insects
- Media
- Instruments
- Room air handling system
- References
- Chapter 6. Callus induction
- Abstract
- Chapter Outline
- Callus initiation
- Explant orientation
- Establishment of competent cereal cell cultures
- Salt selection in vitro
- Growth curves
- Cellular variation from callus cultures
- References
- Further reading
- Chapter 7. Regeneration and morphogenesis
- Abstract
- Chapter Outline
- Controlled morphogenesis: potato tuberization
- Somatic embryogenesis
- Regeneration of rice
- Dormancy requirements of explants
- Primary morphogenesis: Douglas fir
- References
- Further reading
- Chapter 8. Woody shrubs and trees
- Abstract
- Chapter Outline
- Rhododendrons and azaleas
- Roses
- Birch trees
- White cedar (arborvitae)
- References
- Chapter 9. Haploid plants from anther culture
- Abstract
- Chapter Outline
- Datura anther culture
- African violet
- Tobacco
- Anther squash technique
- References
- Further reading
- Chapter 10. Embryo rescue
- Abstract
- Chapter Outline
- Sweet corn embryo culture
- Crabapple and pear
- References
- Further reading
- Chapter 11. Meristem culture for virus-free plants
- Abstract
- Chapter Outline
- Isolation of the shoot apical meristem
- Establishing virus- and bacteria-free plants
- Garlic propagation from bulb scales
- References
- Further reading
- Chapter 12. In vitro propagation for commercial production of ornamentals
- Abstract
- Chapter Outline
- Boston fern
- Staghorn fern
- Ficus
- Kalanchoe
- African violet
- Endangered species
- Cactus
- References
- Further reading
- Chapter 13. Protoplast isolation and fusion
- Abstract
- Chapter Outline
- Corn
- Tomato
- Other plant protoplast isolation and fusion
- References
- Further reading
- Chapter 14. Agrobacterium-mediated transformation of plants
- Abstract
- Chapter Outline
- Petunia or tobacco leaf disk
- Tomato cotyledon/hypocotyl or lettuce leaf disk
- Petunia shoot apex
- Mature rice seed-derived callus
- Arabidopsis floral dip
- Tobacco leaf infiltration
- References
- Further reading
- Chapter 15. Genome editing and RNA interference technologies in plants
- Abstract
- Chapter Outline
- Genome editing using a CRISPR/Cas9 or TALEN system
- RNA interference technology to suppress gene expression
- References
- Further reading
- Appendix I. Useful measurements
- Length
- Temperature
- Concentration
- Weight
- Appendix II. Review of solution preparation
- Percentage solutions
- Molar solutions
- Dilution of a stock solution
- Appendix III. List of suppliers
- Appendix IV. Common plant tissue culture terms
- Index
- No. of pages: 252
- Language: English
- Edition: 4
- Published: February 17, 2021
- Imprint: Academic Press
- Paperback ISBN: 9780128211205
- eBook ISBN: 9780323851374
SP
Sunghun Park
Sunghun Park is Professor at the Department of Horticulture and Natural Resources at Kansas State University, USA. He earned his PhD in Plant Physiology from Texas A&M University in 1995 and his main research areas include abiotic stress physiology, crop improvement using plant tissue culture and genome editing technology.
Affiliations and expertise
Professor, Department of Horticulture and Natural Resources, Kansas State University, Manhattan, KS, USARead Plant Tissue Culture on ScienceDirect