Plant Molecular Biology LabFax
- 1st Edition - January 1, 1993
- Imprint: Academic Press
- Editors: R. R.D Croy, Ali D. Hames, D. Rickwood
- Language: English
- eBook ISBN:9 7 8 - 0 - 0 8 - 0 9 1 7 5 3 - 5
LABFAX volumes are purpose-designed data reference books for practicing scientists. Each book presents key information for a major subject in one place and so saves hours of… Read more
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Request a sales quoteLABFAX volumes are purpose-designed data reference books for practicing scientists. Each book presents key information for a major subject in one place and so saves hours of searching. It does not simply collect together data which are already available in catalogs, since these are often incomplete and can contain conflicting information. Rather, the authors and editors of each LABFAX volume have searched the original literature for the accurate data which they know the specialist needs.
Plant Molecular Biology Labfax is a detailed compendium of essential information on plant nucleic acids, transformation and expression vectors, selectable genes and reporter genes, gene expression and PCR techniques, etc. A key feature is the Plant Gene Index, comprising comprehensive tables of plant genes published and submitted to sequence databases.
Plant Molecular Biology Labfax, while specializing in molecular aspects of plant science, inevitably has numerous sections dealing with general molecular biology which complement the extensive information provided in Molecular Biology Labfax. It is therefore a worthy companion to this text.
Plant Molecular Biology Labfax is a detailed compendium of essential information on plant nucleic acids, transformation and expression vectors, selectable genes and reporter genes, gene expression and PCR techniques, etc. A key feature is the Plant Gene Index, comprising comprehensive tables of plant genes published and submitted to sequence databases.
Plant Molecular Biology Labfax, while specializing in molecular aspects of plant science, inevitably has numerous sections dealing with general molecular biology which complement the extensive information provided in Molecular Biology Labfax. It is therefore a worthy companion to this text.
Contributors
Abbreviations
1. Plant Anatomy, Histology and Taxonomy for Molecular Biologists
Histology: Plant Cell Types
Parenchyma
Epidermis
Mesophyll
Endodermis
Pericycle
Collenchyma
Sclerenchyma
Vascular bundles
Xylem
Phloem
Cambium
Anatomy
Stem
Root
Leaf
Flower
Fruits and Seeds
Methods for Examination of Plant Tissues
Embedding and Sectioning
Histochemical Staining
Fluorescence Microscopy
General Information
Procedure
Commonly Used Fluorochromes
Taxonomy
Rules for the Citation of Plant Names in Scientific Publications
Nomenclature of Major Crop and Experimental Plants
References
Further Reading
2. Plant Nucleic Acids
DNA Isolation from Plants
Isolation of Agrobacterium Ti Plasmid DNA
Calculation of Gene Copy Number Equivalents
Formula
Nomogram
Computer Program
RNA Isolation from Plants
Codon Usage
References
3. Plant Gene Index
Introduction
References
4. Plant Transformation and Expression Vectors
Introduction
Expression Cassettes
pRT103
pJIT74
pDH51
pJIT60
pMA406
pKYLX3
PJIT117
Reporter Genes
Gus Gene
Cat and Luc Genes
Other Reporter Genes
Selectable Markers
Kanamycin Resistance
Hygromycin Resistance
Bialaphos Resistance
Sulfonamide Resistance
Streptomycin/Spectinomycin Resistance
Chlorsulfuron Resistance
Methotrexate Resistance
Phenotypic Markers
Anthocyanin Regulatory Genes
Binary Vectors
pBIN19
pROK1
pGA482
pCGN1547, pCGN1548
pUCD2340
pC22
References
5. Plant Selectable Genes, Reporter Genes and Promoters
Introduction
Aminoglycoside Adenyltransferase Gene [S]
Bleomycin-Resistance Gene [S]
Cauliflower Mosaic Virus 35S Promoter [P]
Cauliflower Mosaic Virus 35S Promoter and Terminator [P,T]
Chloramphenicol Acetyltransferase Gene [R]
Dihydrofolate Reductase [S]
Dihydropteroate Synthase Gene [S]
Enolpyruvylshikimate Phosphate Synthase Gene [S]
β-Galactosidase Gene [RH]
β-Glucuronidase Gene [RH]
Hygromycin Phosphotransferase Gene [S]
Luciferase Gene [R]
Luciferase A and B Genes [R]
Neomycin Phosphotransferase Gene [S, R]
Nopaline Synthase Gene [R, P, T]
Octopine Synthase Gene [R, P, T]
Phosphinothricin Acetyltransferase Gene [S]
References
6. Characteristics of Plant Pre-mRNA Introns and Transposable Elements
Introduction
Mobile Genetic Elements in Higher Plants
References
7. Plant Tissue Culture and Genetic Transformation
Tissue Culture Media
Common Basal Media
Media Supplements
Media and Methods for the Culture of Tobacco
Genetic Transformation
Gene Transfer Methods and their Evaluation
Stably Transformed Plant Species
Agrobacterium Strains, Media and Methods
Protoplast Transformation
Particle Bombardment of Tissues
Examples of Tissue-Specific Transient Expression
References
8. Plant Organellar Targeting Sequences
Nucleus
Peroxisomes/Glyoxysomes (Microbodies)
Secretion Pathway
Mitochondria
Chloroplasts
Other Plastids
References
9. Chloroplast Molecular Biology
Introduction
What cpDNA Encodes
Nomenclature of Genes and their Products
Genes for the Polypeptides of Multi-Component Thylakoid Complexes Involved in Electron Transport
Photosystem I (PSI) Plastocyanin: Ferredoxin Oxidoreductase
Photosystem II (PSII) Water: Plastoquinone Oxidoreductase
Cytochrome Blf Complex (Plastoquinol: Plastocyanin Oxidoreductase)
ATP Synthase (F0F1-ATPase, EC 3.6.1.34)
NADPH: Plastoquinone Oxidoreductaste (NADPH Dehydrogenase)
The Chloroplast Genetic System
Ribosomal RNA
Chloroplast Ribosomal Proteins
tRNA Genes
RNA Polymerase
Chloroplast Gene Expression
Transcription
Introns, Splicing and Processing
Chloroplast Genome Structure
Gene Arrangements
Chloroplast Transformation
Transformation of Plastids of Higher Plants
Transformation of the Chloroplast of Chlamydomonas reinhardtii
Markers for Selection of Transformants in C. reinhardtii
Apparatus for Particle Bombardment
References
10. Plant Gene Expression and PCR Techniques
PCR Strategies
Assay of Plant Gene Expression
Introduction
References
Index
- Edition: 1
- Published: January 1, 1993
- Imprint: Academic Press
- Language: English
- eBook ISBN: 9780080917535
RC
R. R.D Croy
Affiliations and expertise
University of Durham, Science Laboratories, Durham, U.K.AH
Ali D. Hames
Affiliations and expertise
Consultant Microbiologist, Freeman Hospital, Newcastle upon Tyne, UKDR
D. Rickwood
Affiliations and expertise
University of Essex, Wivenhoe Park, ColchesterRead Plant Molecular Biology LabFax on ScienceDirect