Chapter 1. Introduction and overview (K. Janssens, R. Van Grieken). Chapter 2. Ultraviolet, infrared and X-ray imaging(F. Mairinger). Chapter 3. Electron microscopy and its role in cultural heritage studies (A. Adriaens, M.G. Dowsett). Chapter 4. X-ray based methods of analysis (K. Janssens). Chapter 5. Ion beam microanalysis (T. Calligaro et al.). Chapter 6. X-Ray photoelectron and Auger electron spectroscopy (A. Hubin, H. Terryn). Chapter 7. Laser ablation inductively coupled plasma mass spectrometry (T.E. Jeffries). Chapter 8. Infrared, Raman microscopy and Fibre-Optic Raman Spectroscopy (FORS) (G.M. Howell et al.). Chapter 9. Secondary ion mass spectrometry. Application to archaeology and art objects (E. Darque-Ceretti1, M. Aucouturier). Chapter 10. The non-destructive investigation of copper alloy patinas (D.A. Scott). Chapter 11. Precious metals artifacts (G. Demortier). Chapter 12. Diagnostic methodology for the examination of Byzantine frescoes and icons. Non destructive investigation and pigment identification (Sister Daniilia et al.). Chapter 13. The provenance of medieval silver coins: analysis with EDXRF, SEM/EDX and PIXE (R. Linke et al.). Chapter 14. Pigment identification in illuminated manuscripts (P. Vandenabeele, L. Moens). Chapter 15. Provenance analysis of glass artefacts (B. Gratuze, K. Janssens). Chapter 16. Corrosion of historic glass and enamels (M. Schreiner). Chapter 17. A study of ancient manuscripts exposed to iron-gall ink corrosion (E. Bulska, B. Wagner).