Myeloid-Derived Suppressor Cells

1st Edition, Volume 184 - March 19, 2024
Editors: Carlos Jiménez-Cortegana, Lorenzo Galluzzi
Language: English
Hardback ISBN:
9 7 8 - 0 - 4 4 3 - 2 1 8 0 7 - 1
eBook ISBN:
9 7 8 - 0 - 4 4 3 - 2 1 8 0 8 - 8

Myeloid-Derived Suppressor Cells, Volume 184 in the Methods in Cell Biology series includes comprehensive protocols to assess the role of myeloid-derived suppressor cells (MDSCs)… Read more

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Myeloid-Derived Suppressor Cells, Volume 184 in the Methods in Cell Biology series includes comprehensive protocols to assess the role of myeloid-derived suppressor cells (MDSCs), an immature, heterogenous cell population from the myeloid lineage with a potent immunosuppressive activity in a variety of diseases. This volume includes interesting protocols that address the Detection of myeloid-derived suppressor cells by flow cytometry, Determination of murine myeloid-derived suppressor cells by flow cytometry, Isolation of Myeloid-Derived Suppressor Cells (MDSC) from Endometriotic Mice Model and their Immunomodulatory Functions, Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood, and much more.

Additional chapters cover Assessment of myeloid-derived suppressor cell differentiation ex vivo, Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo, Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes, Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells, In Vitro Osteoclastogenesis Assessment using Murine Myeloid-derived Suppressor Cells, Estimating Nitric Oxide (NO) from MDSCs by Griess Method, and In vitro generation of myeloid-derived suppressor cells (MDSCs) from hematopoietic progenitor cells (HPCs).

Cover image
Title page
Table of Contents
Series Page
Copyright
Contributors
Chapter 1 Detection of myeloid-derived suppressor cells by flow cytometry
Abstract
1 Introduction
2 Materials
3 Methods
4 Notes
5 Conclusion
References
Chapter 2 Flow cytometric detection of CD11b+ Gr-1+ cells in nontumor-bearing mice: A propolis-elicited model
Abstract
1 Introduction
2 Materials (see Note 1)
3 Methods
4 Notes
5 Results
6 Conclusions
References
Chapter 3 Isolation of myeloid-derived suppressor cells (MDSC) from endometriotic mice model and their immunomodulatory functions
Abstract
1 Introduction
2 Materials
3 Methods
4 Notes
5 Concluding remarks
References
Chapter 4 Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood
Abstract
1 Introduction
2 Materials
3 Methods
4 Notes
5 Concluding remarks
References
Chapter 5 CD4+ T cells proliferation assay to analyze Mo-MDSCs suppressive function
Abstract
1 Introduction
2 Materials
3 Methods
4 Notes
5 Concluding remarks
References
Chapter 6 Assessment of myeloid-derived suppressor cell differentiation ex vivo
Abstract
1 Introduction
2 Materials
3 Methods
4 Notes
5 Concluding remarks
References
Further reading
Chapter 7 Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo
Abstract
1 Introduction
2 Materials
3 Methodology
References
Chapter 8 Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes
Abstract
1 Introduction
2 Materials
3 Method
4 Notes
5 Conclusion
Acknowledgment
References
Chapter 9 Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells
Abstract
1 Introduction
2 Materials
3 Methods
4 Notes
References
Chapter 10 In vitro osteoclastogenesis assessment using murine myeloid-derived suppressor cells
Abstract
1 Introduction
2 Materials
3 Methods
4 Notes
5 Concluding remarks
References
Chapter 11 Estimating nitric oxide (NO) from MDSCs by Griess method
Abstract
1 Introduction
2 Materials
3 Methods
4 Concluding remarks
References
Chapter 12 In vitro generation of murine myeloid-derived suppressor cells from hematopoietic progenitor cells
Abstract
1 Introduction
2 Methods
Acknowledgments
References

Carlos Jiménez-Cortegana

Carlos Jiménez-Cortegana obtained the degree in Chemistry at University of Seville (Spain) in 2014. In July 2020 he got the PhD in Clinical Research at the same University, belonging to the group headed by Dr. Luis de la Cruz-Merino and Dr. Víctor Sánchez-Margalet. Currently, Carlos is a Postdoctoral Researcher at the Department of Radiation Oncology, Weill Cornell Medical College (New York, USA), in the research group headed by Dr. Lorenzo Galluzzi. From 2021, Carlos has published more than 15 articles in recognized, international journals, some of them in high-impact factor journals, mainly focused on the immunosuppressive role of myeloid-derived suppressor cells in a variety of diseases, including breast cancer, diffuse large B-cell lymphoma, and COVID-19.

Affiliations and expertise

Radiation Oncology, Weill Cornell Medical College, New York, NY, USA; Department of Medical Biochemistry, Molecular Biology and Immunology, Faculty of Medicine, University of Seville, Seville, Spain

Lorenzo Galluzzi

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Affiliations and expertise

Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA