Monitoring Vesicular Trafficking in Cellular Responses to Stress
- 1st Edition, Volume 164 - June 30, 2021
- Editors: Oliver Kepp, Lorenzo Galluzzi
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 8 2 3 5 4 4 - 7
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 3 5 4 5 - 4
Monitoring Vesicular Trafficking in Cellular Responses to Stress, Volume 164 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume pre… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quoteMonitoring Vesicular Trafficking in Cellular Responses to Stress, Volume 164 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics. Each chapter is written by an international board of authors.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Cell Biology series
- Includes the latest information on the topic of Monitoring vesicular trafficking in cellular responses to stress
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Vesicular trafficking in cellular responses to stress
- Chapter 1: Monitoring TFEB translocation
- Abstract
- 1: Introduction
- 2: Disposable material
- 3: Equipment
- 4: Reagents
- 5: Cell culture and passage
- 6: Immunofluorescence
- 7: Concluding remarks
- 8: Notes
- Acknowledgment
- Chapter 2: Monitoring lipophagy in kidney epithelial cells in response to shear stress
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Conclusion
- 5: Notes
- Acknowledgments
- Chapter 3: Live cell imaging of LC3 dynamics
- Abstract
- 1: Introduction
- 2: Material
- 3: Methods
- 4: Concluding remarks
- 5: Notes
- Chapter 4: Autophagy assessment in circulating leukocytes
- Abstract
- 1: Introduction
- 2: Material
- 3: Induction of autophagy by starvation in mice and blood collection
- 4: Leukocytes isolation, fixation and permeabilization
- 5: Sample preparation and immunostaining
- 6: Image flow cytometric acquisition and data analysis
- 7: Notes
- 8: Concluding remarks
- Acknowledgments
- Chapter 5: Measuring lysosomal size and frequency by electron microscopy
- Abstract
- 1: Introduction
- 2: Material
- 3: Workflow
- 4: Concluding remarks
- Notes
- Acknowledgment
- Chapter 6: Autophagic flux assessment by immunoblot
- Abstract
- 1: Introduction
- 2: Material
- 3: Concluding remarks
- 4: Notes
- Chapter 7: Assessing autophagic flux in yeast
- Abstract
- 1: Introduction
- 2: GFP liberation assay
- 3: ALP activity assay
- 4: Concluding remarks
- 5: Notes
- Chapter 8: Multiple analysis of mitochondrial metabolism, autophagy and cell death
- Abstract
- 1: Introduction
- 2: Cell death assays
- 3: Autophagy monitoring
- 4: Mitochondrial network analysis
- 5: Real time bioenergetic profile analysis
- Notes
- Acknowledgments
- Chapter 9: Quantitative determination of mitophagy by fluorescence microscope
- Abstract
- 1: Introduction
- 2: Material
- 3: GFP-LC3 and MitoTracker red approach
- 4: Concluding remarks
- Acknowledgments
- Chapter 10: Quantifying phagosomal HOCl at single immune-cell resolution
- Abstract
- 1: Introduction
- 2: Protocol
- 3: Summary
- Acknowledgments
- Chapter 11: The intracellular metabolome of starving cells
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Quality control
- 5: Concluding remarks
- 6: Notes
- Chapter 12: Quantitative determination of autophagy flux by probes
- Abstract
- 1: Introduction
- 2: Material
- 3: Concluding remarks
- 4: Notes
- Chapter 13: Assessment of mammalian endosomal microautophagy
- Abstract
- 1: Introduction
- 2: Biochemical procedures for the assessment of eMI activity in mammals
- 3: Image-based procedures for assessment of eMI activity in cultured cells
- 4: Criteria to identify a protein as potential eMI substrate
- 5: Concluding remarks
- Acknowledgments
- Chapter 14: Measuring oxidation within LC3-associated phagosomes that optimizes MHC class II restricted antigen presentation
- Abstract
- 1: Introduction
- 2: Materials
- 3: Protocol I: Measurement of the oxidation level within LAPosomes in human macrophages
- 4: Protocol II: Antigen presentation assay using whole blood CD4+ T cells
- 5: Concluding remarks
- 6: Notes
- Acknowledgments
- No. of pages: 222
- Language: English
- Edition: 1
- Volume: 164
- Published: June 30, 2021
- Imprint: Academic Press
- Hardback ISBN: 9780128235447
- eBook ISBN: 9780128235454
OK
Oliver Kepp
Dr. Oliver Kepp graduated from Humboldt University Berlin and obtained a PhD degree in Molecular Biology. Today he is a tenured researcher at INSERM and a Co-director of the BioCell automation platform at GRCC. Oliver and his team investigate several aspects of tumor immunogenicity, focusing on systems cell biology approaches. He is also a co-founder of Samsara therapeutics and was appointed associate director of the European academy of tumor immunology in 2019. Oliver received the prestigious Clarivate Highly Cited Researcher Award four years in a row since 2018 and has recently been ranked among the top biology and biochemistry scientists in France (#5; www.research.com)
Affiliations and expertise
Equipe labellisée par la Ligue contre le cancer, Université de Paris, Sorbonne UniversitéLG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USARead Monitoring Vesicular Trafficking in Cellular Responses to Stress on ScienceDirect