Molecular Regulation of Nuclear Events in Mitosis and Meiosis

Contents

Preface


1 Development of Cytoplasmic Activities That Control Chromosome Cycles during Maturation of Amphibian Oocytes

I. Introduction

II. Oocyte Maturation

III. Maturation-Promoting Factor (MPF)

IV. Cytostatic Factor (CSF)

V. Concluding Remarks

References


2 Dynamics of the Nuclear Lamina during Mitosis and Meiosis

I. Introduction

II. Dynamics of the Nuclear Lamina in Mitosis

III. Dynamics of the Nuclear Lamina during Meiotic Prophase

IV. Dynamics of the Nuclear Lamina during Egg Maturation and Early Development

V. Comparison of the Dynamics of the Nuclear Lamina during Mitosis and Meiosis

References


3 Regulation of Nuclear Formation and Breakdown in Cell-Free Extracts of Amphibian Eggs

I. Introduction

II. Regulation of Pronuclear Formation

III. Regulation of Chromosome Condensation

IV. Role of MPF, Ca2+ Ions, and Protein Phosphorylation in Controlling Nuclear Envelope Breakdown, Chromosome Condensation, and Spindle Assembly in Cell-Free Extracts

V. Summary

References


4 Role of Protein Phosphorylation in Xenopus Oocyte Meiotic Maturation

I. Introduction

II. Maturation-Inhibiting Phosphoproteins (Mp-P) and cAMP

III. Protein Phosphorylation and Maturation-Promoting Factor (MPF) Activity

IV. Conclusions

References


5 Maintenance of Oocyte Meiotic Arrest by Follicular Fluid Factors

I. Introduction

II. Characterization of Follicular Fluid Oocyte Maturation Inhibitor (OMI) by Use of Isolated Mammalian Oocytes

III. Hypoxanthine in Pig Follicular Fluid as the Principal Inhibitor of Mouse Oocyte Maturation and the Prevention of Follicle Stimulating Hormone (FSH) Reversal of cAMP-Maintained Meiotic Arrest

IV. Assay for and Characterization of Human Follicular Fluid OMI by Use of Oocytes from the Amphibian, Xenopus laevis

V. Failure to Inhibit Xenopus Oocyte Maturation by Use of Exogenous Hypoxanthine in Combination with N6,2'-0-Dibutyryl-cAMP (Bt2-Cyclic AMP)

VI. How Do the Inhibitory Factors Present in Follicular Fluid Maintain Meiotic Arrest in Oocytes?

VII. Conclusions

References


6 Regulation of Chromatin Condensation and Decondensation in Sea Urchin Pronuclei

I. Introduction

II. Mitotic and Premature Chromosome Condensation

III. Male Pronuclear Decondensation

IV. Conclusions

References


7 Regulation of Mitosis by Nonhistone Protein Factors in Mammalian Cells

I. Introduction

II. The Mitotic Factors

III. Inhibitors of the Mitotic Factors

IV. Role of Protein Phosphorylation and Dephosphorylation in the Regulation of Nuclear Events Associated with Mitosis and Meiosis

V. Conclusions

References


8 Mitosis-Specific Cytoplasmic Protein Kinases

I. Introduction

II. Identification of Protein Kinases in Cellular Extracts

III. Cell Cycle Specificity of Kinase Activities

IV. Comparison of Kinase Activities with Protein Kinases Implicated in Mitotic Events

V. Comparison of Kinase Activities with Mitotic Factors

VI. Conclusions and Perspectives

References


9 Antibodies to Mitosis-Specific Phosphoproteins

I. Introduction

II. Monoclonal Antibodies to Cells in Mitosis

III. Characterization of Antigens Reactive with MPM-1 and MPM-2

IV. Cytological Localization of Antigens Reactive with MPM-1 and MPM-2

V. Biological Activity of MPM-1 and MPM-2 Antibodies

VI. Discussion

References


10 Mitosis-Specific Protein Phosphorylation Associated with Premature Chromosome Condensation in a ts Cell Cycle Mutant

I. Introduction

II. Isolation and Preliminary Characterization of the tsBN2 Cell Line

III. Induction of Premature Chromosome Condensation (PCC) in tsBN2 Cells at the Nonpermissive

Temperature

IV. Induction of Mitosis-Specific Phosphorylation in tsBN2 Cells by Temperature Shift

V. Newly Synthesized Protein(s) in tsBN2 Cells Showing PCC

VI. Discussion

References


11 Chromatin Structure and Histone Modifications through Mitosis in Plasmodia of Physarum polycephalum

I. Introduction

II. Chromatin Structure and Organization

III. Control of Chromosome Condensation

IV. Cell Cycle Studies of Histone Modifications

V. Histone Hl°

VI. Histone HI Kinases

VII. Discussion

References

Index