Molecular Players in iPSC Technology
- 1st Edition - August 29, 2021
- Imprint: Academic Press
- Editor: Alexander Birbrair
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 0 0 5 9 - 1
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 0 0 6 0 - 7
The series Advances in Stem Cell Biology is a timely and expansive collection of comprehensive information and new discoveries in the field of stem cell biolog… Read more
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Request a sales quoteThe series Advances in Stem Cell Biology is a timely and expansive collection of comprehensive information and new discoveries in the field of stem cell biology.
Molecular Players in iPSC Technology, Volume 12 addresses the molecular players underlying induced pluripotent stem cell (iPSC) generation, maintenance, expansion, and differentiation.
The discovery of iPSCs revolutionized biomedical research. iPSC technology involves multiple molecular mechanisms. This volume covers exosomal microRNAs, auxiliary pluripotency-associated genes, inducible caspase-9 suicide gene, cell cycle proteins, ion channels, Notch signaling, kinase signaling, SOCS3/JAK2/STAT3 pathway, NANOG, Krüppel-like factors, H1FOO, and much more in iPSCs.
The volume is written for researchers and scientists in stem cell therapy, cellular and molecular biology, and regenerative medicine and is contributed by world-renowned authors in the field.
Molecular Players in iPSC Technology, Volume 12 addresses the molecular players underlying induced pluripotent stem cell (iPSC) generation, maintenance, expansion, and differentiation.
The discovery of iPSCs revolutionized biomedical research. iPSC technology involves multiple molecular mechanisms. This volume covers exosomal microRNAs, auxiliary pluripotency-associated genes, inducible caspase-9 suicide gene, cell cycle proteins, ion channels, Notch signaling, kinase signaling, SOCS3/JAK2/STAT3 pathway, NANOG, Krüppel-like factors, H1FOO, and much more in iPSCs.
The volume is written for researchers and scientists in stem cell therapy, cellular and molecular biology, and regenerative medicine and is contributed by world-renowned authors in the field.
- Provides overview of the fast-moving field of iPSC technology, regenerative medicine, and therapeutics
- Covers the different key molecular players involved in iPSC formation, maintenance, expansion, and differentiation
- Is contributed by world-renowned experts in the field
Researchers and scientists in stem cell therapy, cell biology, regenerative medicine, and organ transplantation. Graduate and undergraduate students in the above fields
- Cover image
- Title page
- Table of Contents
- Advances in Stem Cell Biology
- Copyright
- Dedication
- Contributors
- About the editor
- Preface
- Chapter 1. Engineering exosomal microRNAs in human pluripotent stem cells
- Introduction
- The role of miRNAs in stem cell properties and disease development
- Key signaling pathways regulated by miRNAs in stem cells
- The role of exo-miRNAs derived from PSCs and MSCs
- Engineering exo-miRNAs produced by stem cells
- Conclusions and future directions
- Chapter 2. Auxiliary pluripotency-associated genes and their contributions in the generation of induced pluripotent stem cells
- Introduction
- Esrrb
- Sall4
- Rex1
- Tbx3
- Utf1
- Zscan4
- Nr5a2
- Glis1
- L-Myc
- Zic3
- Foxh1
- Conclusion
- Chapter 3. Improving the safety of iPSC-derived T cell therapy
- Introduction
- Generation and use of iPSC-derived antigen-specific CTLs
- Chimeric antigen receptor T cells generated from iPSCs
- Increasing the safety of iPSC
- Suicide-gene-based safeguard system
- iC9-based safeguard system for iPSC-derived cell therapy
- CART cell therapy with iC9 safeguard system
- Banking iPSCs for various HLA types
- “Off-the-shelf” T cell therapy and GvHD prevention
- Genome-edited T cell therapy and protection from missing-self response of NK cells
- Prospects for rejuvenated CTL therapy
- Chapter 4. Induced pluripotency and intrinsic reprogramming factors: adult stem cells versus somatic cells
- Introduction
- Multiple factors/genes responsible for reprogramming/inducing pluripotency-library screening
- Signaling pathways that enhance pluripotency during iPSCs generation
- Wnt/β-catenin, TGF-β, and hippo signaling pathways
- The ubiquitin-proteasome system
- Epithelial-to-mesenchymal transition and mesenchymal-to-epithelial transition
- Methods for induction of pluripotency
- Integrative method
- Lentiviral vectors
- Transfection using linear DNA
- Nonintegrative methods
- Reprogramming using small molecules
- Cell types with intrinsic reprogramming factors and their conversion into iPSC
- Possible role and use of epigenetic modifiers in pluripotency induction
- Histone H3 Lysine 9 Methylation
- Histone H3 Lysine 79 (H3K79) Methylation
- Methylation at histone 3 lysine 36 2/3
- Histone Deacetylation
- Induced pluripotency and gene incorporation—safety and efficacy issues
- Cell therapy using induced pluripotent stem cells
- Conclusion
- Chapter 5. The role of cell cycle in reprogramming toward induced pluripotent stem cells (iPSCs)
- Preface
- Introduction
- G1-phase: main regulators and reprogramming
- S-phase/G2 and reprogramming
- p53 and other tumor suppressors
- Cip/Kip family and reprogramming to pluripotency
- Future directions and perspectives
- Chapter 6. Signaling pathways regulate cardiovascular lineage commitment of hPSCs
- Human pluripotent stem cells
- Cardiovascular development in the heart
- Cardiac differentiation of hPSCs
- Maturation of cardiomyocytes derived from hPSCs
- Nonmyocytes derived from cardiac progenitors
- HPSCs-derived cardiomyocytes used for disease modeling and drug screening platforms
- HPSCs-derived cardiomyocytes for cellular therapy applications
- Current challenges and future directions
- Chapter 7. Role of ion channels in human induced pluripotent stem cells–derived cardiomyocytes
- Introduction
- Phases of the cardiac action potential
- The ionic basis underlying hiPSC cardiomyocyte action potential
- The ionic basis underlying hiPSC cardiomyocyte action potential
- Transformation of hiPSC to the cardiac lineage
- Strategies to enhance maturation
- Summary and future directions
- Chapter 8. Notch signaling in induced pluripotent stem cells
- Introduction
- Notch ligands and receptors
- Canonical notch signaling
- Noncanonical notch signaling
- Receptor- and ligand-dependent notch signaling
- Notch signaling interaction with other pathways
- Notch signaling in stemness maintenance and differentiation
- Notch signaling in induced pluripotent stem cells
- Conclusion
- Chapter 9. The extracellular signal-regulated kinase signaling pathway in biology of pluripotent stem cells
- Introduction
- ERK signaling in somatic cell reprogramming
- ERK signaling in cardiovascular differentiation of pluripotent stem cells
- Conclusions
- Chapter 10. SOCS3/JAK2/STAT3 pathway in iPSCs
- Introduction
- Main text
- Conclusions and future perspectives
- Chapter 11. Nanog in iPS cells and during reprogramming
- Introduction
- Nanog discovery, function, and structure
- Nanog multilayered regulation
- Uncovering Nanog's connection to cell reprogramming
- Reprogramming enhancement by Nanog affects efficiency and quality of iPS cells
- Nanog is associated to multiple reprogramming roadblocks
- Chromatin remodeling linked to Nanog function is relevant during reprogramming
- Nanog reactivation is a common feature between reprogramming and some oncogenic processes
- Concluding remarks
- Chapter 12. The role of Krüppel-like factors in generating induced pluripotent stem cells
- Overview of KLFs
- Protein structures of KLFs
- KLF4 and discovery of iPSC
- Substitution of KLF4 by KLF2 and KLF5 in reprogramming
- KLFs as members of autoregulated transcriptional network for stem cell identity
- Dual role of KLF4 in a stepwise model of reprogramming
- Efficiency of reprogramming as determined by stoichiometry
- Reprogramming without exogenous KLFs
- KLFs contributes to naïve pluripotency
- Future direction: KLF interactome and the study of structure–function relationship
- Chapter 13. The oocyte-specific linker histone H1FOO plays a key role in establishing high-quality mouse induced pluripotent stem cells
- Issues surrounding induced pluripotent stem cells
- Oocyte components can promote somatic cell reprogramming
- Characteristics of linker histone H1
- Oocyte-specific linker histone H1FOO is involved in open chromatin formation
- Ectopic expression of H1foo in somatic cells enhances qualified iPSC generation
- Transcript expression and methylation characteristics of OSKH-iPSCs
- H1foo promotes in vitro and in vivo differentiation potentials of iPSCs
- Future directions
- Index
- Edition: 1
- Published: August 29, 2021
- No. of pages (Paperback): 436
- No. of pages (eBook): 436
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9780323900591
- eBook ISBN: 9780323900607
AB
Alexander Birbrair
Dr. Alexander Birbrair received his bachelor’s biomedical degree from Santa Cruz State University in Brazil. He completed his PhD in Neuroscience, in the field of stem cell biology, at the Wake Forest School of Medicine under the mentorship of Osvaldo Delbono. Then, he joined as a postdoc in stem cell biology at Paul Frenette’s laboratory at Albert Einstein School of Medicine in New York. In 2016, he was appointed faculty at Federal University of Minas Gerais in Brazil, where he started his own lab. His laboratory is interested in understanding how the cellular components of different tissues function and control disease progression. His group explores the roles of specific cell populations in the tissue microenvironment by using state-of-the-art techniques. His research is funded by the Serrapilheira Institute, CNPq, CAPES, and FAPEMIG. In 2018, Alexander was elected affiliate member of the Brazilian Academy of Sciences (ABC), and, in 2019, he was elected member of the Global Young Academy (GYA), and in 2021, he was elected affiliate member of The World Academy of Sciences (TWAS). He is the Founding Editor and Editor-in-Chief of Current Tissue Microenvironment Reports, and Associate Editor of Molecular Biotechnology. Alexander also serves in the editorial board of several other international journals: Stem Cell Reviews and Reports, Stem Cell Research, Stem Cells and Development, and Histology and Histopathology.
Affiliations and expertise
Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
Department of Radiology, Columbia University Medical Center, Medical Center, USARead Molecular Players in iPSC Technology on ScienceDirect