
Modern Applications of Plant Biotechnology in Pharmaceutical Sciences
- 1st Edition - July 22, 2015
- Imprint: Academic Press
- Authors: Saurabh Bhatia, Kiran Sharma, Randhir Dahiya, Tanmoy Bera
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 8 0 2 2 2 1 - 4
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 0 2 4 9 8 - 0
Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how t… Read more

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explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs.The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding.
Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more.
Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications.
- Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences
- Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis
- Examines critical issues of international importance and offers real-life examples and potential solutions
- About the Lead Author
- Preface
- Chapter 1: History and Scope of Plant Biotechnology
- Abstract
- 1.1. Introduction
- 1.2. History of plant biotechnology
- 1.3. Scope and importance of biotechnology
- Chapter 2: Plant Tissue Culture
- Abstract
- 2.1. Introduction
- 2.2. Types of tissue culture
- 2.3. Plant tissue culture media
- 2.4. Selection of a suitable new medium
- 2.5. Preparation of media
- 2.6. Callus induction, subculture, and maintenance
- 2.7. Optimization of media
- 2.8. Maintenance of cultures cell lines
- 2.9. Assessment of their growth parameters
- Chapter 3: Laboratory Organization
- Abstract
- 3.1. Introduction
- 3.2. Washing area
- 3.3. Media preparation area
- 3.4. Sterilization room
- 3.5. Aseptic transfer area/inoculation room
- 3.6. Culture room
- 3.7. Data collection area
- 3.8. Acclimatization area
- 3.9. Safety rules
- 3.10. Design and layout of tissue culture laboratory
- 3.11. Automation in plant tissue culture
- Chapter 4: Concepts and Techniques of Plant Tissue Culture Science
- Abstract
- 4.1. Introduction
- 4.2. Culturing techniques
- 4.3. Sterilization techniques
- 4.4. Genetic engineering techniques
- 4.5. Screening method of genomic library
- 4.6. Electrophoresis techniques
- 4.7. Polymerase chain reaction
- 4.8. Microarray
- 4.9. Cryopreservation
- 4.10. Autoradiography
- 4.11. Other techniques
- Chapter 5: Application of Plant Biotechnology
- Abstract
- 5.1. Introduction
- 5.2. Plant basic research
- 5.3. Applied research
- Chapter 6: Somatic Embryogenesis and Organogenesis
- Abstract
- 6.1. Introduction
- 6.2. Somatic embryogenesis
- 6.3. Synthetic seed technology
- 6.4. Organogenesis
- Chapter 7: Classical and Nonclassical Techniques for Secondary Metabolite Production in Plant Cell Culture
- Abstract
- 7.1. Introduction
- 7.2. Conventional methods
- 7.3. Modern or nonclassical methods for the production of secondary metabolites
- 7.4. Production of antitumor compounds by plant cell culture
- 7.5. Production of flavoring compounds through tissue culture
- 7.6. Antimicrobial agents from plant cell culture
- Chapter 8: Plant-Based Biotechnological Products With Their Production Host, Modes of Delivery Systems, and Stability Testing
- Abstract
- 8.1. Introduction
- 8.2. Plant-based biotechnological products with their respective production host
- 8.3. Different modes of delivery systems used for plant-based biopharmaceuticals
- 8.4. Stability of proteins and peptides
- Chapter 9: Edible Vaccines
- Abstract
- 9.1. Introduction
- 9.2. Production of edible vaccines
- 9.3. Mechanism of Action
- 9.4. Advantages and disadvantages of edible vaccines
- 9.5. Most popular edible vaccines produced by plants [8,9]
- Chapter 10: Microenvironmentation in Micropropagation
- Abstract
- 10.1. Introduction
- 10.2. Various problems faced during microenvironmentation
- 10.3. Goals of microenvironmentation
- 10.4. Gas microenvironment
- 10.5. Light microenvironment
- 10.6. Water microenvironment
- 10.7. Temperature microenvironment
- 10.8. Physical properties of culture vessels
- 10.9. Evaluation of photosynthetic capacity in micropropagated plants by image analysis
- 10.10. Monitoring gene expression in plant tissue cultures
- 10.11. Bioreactor designs
- 10.12. Integrating automation technologies with commercial micropropagation
- 10.13. Machine vision and robotics for the separation and regeneration of plant tissue culture
- 10.14. Tissue culture gel firmness: measurement and effects on growth
- 10.15. Commercialized photoautotrophic micropropagation system
- 10.16. Closed system for high quality transplants using minimum resources
- 10.17. Other environment control systems
- Chapter 11: Micropropagation
- Abstract
- 11.1. Introduction
- 11.2. Types of explants used in micropropagation
- 11.3. Methods of plantlet production used in micropropagation
- 11.4. Stages involved in micropropagation
- 11.5. Economic significance
- 11.6. Applications and merits of micropropagation over conventional plant breeding [22–24]
- Chapter 12: Laws in Plant Biotechnology
- Abstract
- 12.1. Introduction
- 12.2. Intellectual property right protection
- 12.3. Organization in intellectual property right protection
- 12.4. Controversial patent cases involving traditional knowledge and genetic resources
- Chapter 13: Technical Glitches in Micropropagation
- Abstract
- 13.1. Introduction
- 13.2. Microbial contamination
- 13.3. Gaseous contamination
- 13.4. Cross-culture contamination
- 13.5. Browning of the medium or phenolic exudation
- 13.6. Absence of rooting and acclimatization
- 13.7. Hyperhydricity
- 13.8. Shoot-tip necrosis
- 13.9. Habituation
- 13.10. Tissue proliferation
- 13.11. Detection of the contaminants
- Chapter 14: Plant Tissue Culture-Based Industries
- Abstract
- 14.1. Introduction
- 14.2. International scenario
- 14.3. Plant tissue culture-based industries in India
- 14.4. Recent technologies
- 14.5. Various problems faced during micropropagation
- Subject Index
- Edition: 1
- Published: July 22, 2015
- Imprint: Academic Press
- No. of pages: 452
- Language: English
- Paperback ISBN: 9780128022214
- eBook ISBN: 9780128024980
SB
Saurabh Bhatia
KS
Kiran Sharma
RD
Randhir Dahiya
TB