MicroRNA
From Bench to Bedside
- 1st Edition - July 19, 2022
- Imprint: Academic Press
- Editor: Junjie Xiao
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 8 9 7 7 4 - 7
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 8 8 5 5 8 - 4
MicroRNA: From Bench to Bedside provides an in-depth, expansive overview of microRNA from fundamentals to clinical practice. It presents researchers with detailed insights… Read more
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Request a sales quoteMicroRNA: From Bench to Bedside provides an in-depth, expansive overview of microRNA from fundamentals to clinical practice. It presents researchers with detailed insights on the topic of microRNA, exploring foundational knowledge that is followed by methodologies and the latest technologies for research and potential theragnostic and therapeutic applications for specific diseases. The book consists of eight parts, beginning with an introduction to microRNA and the current state of the field, followed by sections on biogenesis and maturation of microRNA, and methodology and bioinformatics, where chapters focus on isolation and detection techniques.
Sections then move on to molecular mechanisms and gene regulation, considering topics such as transcriptional regulation and epigenetic regulation, as well as the role of microRNA as biomarkers. Additionally, microRNA and human disease and microRNA-based therapeutics are explored, focusing on a wide range of diseases such as cancer, age-related disease, cardiovascular disease, microRNA targeted therapy in hepatitis and therapeutic strategies for diabetes. The book concludes with a discussion on advances and future perspectives in microRNA investigation.
- Covers the topic of microRNA in detail, from foundational knowledge to clinical application
- Explores the physiological and pathological roles of microRNAs in various human diseases, including neurological, cardiovascular and age-related diseases
- Discusses future directions and challenges in the field
- Includes chapters on methodology and bioinformatics for microRNA research
Researchers in molecular biology, biochemistry, cell biology, and medicine; Molecular biologists, biochemists, cell biologists, translational researchers and clinicians. Pathologists and geneticists; students and graduate students in related disciplines
- Cover image
- Title page
- Table of Contents
- Copyright
- List of contributors
- Preface
- Part I: Overview
- Chapter 1. Overview of micro-RNA
- Abstract
- 1.1 Introduction
- 1.2 The biogenesis of micro-RNA
- 1.3 Roles of micro-RNA in cancer
- 1.4 micro-RNAs and chemoresistance/chemosensitivity
- 1.5 micro-RNA and SARS-CoV-2
- 1.6 micro-RNA in immune system
- 1.7 micro-RNA and autoimmune
- 1.8 micro-RNA and aging
- 1.9 micro-RNAs and other diseases
- 1.10 micro-RNA biogenesis of plant
- 1.11 Cross talking of micro-RNA
- 1.12 Conclusion
- References
- Part II: Biogenesis and maturation
- Chapter 2. Maturation of microRNAs
- Abstract
- 2.1 Introduction
- 2.2 The classic canonical miRNA maturation pathway
- 2.3 The alternative noncanoniocal miRNA maturation pathway
- 2.4 The commonly used approaches to study miRNA maturation
- References
- Part III: Methodology and bioinformatics
- Chapter 3. MicroRNA interference
- Abstract
- 3.1 Background: definition and applications
- 3.2 Methods
- 3.3 Conclusion
- References
- Chapter 4. MicroRNA target prediction and validation
- Abstract
- 4.1 Introduction
- 4.2 Biological concepts used in miRNA target predictions
- 4.3 Available miRNA target prediction algorithms
- 4.4 Common experimental validation methods of miRNA targets
- 4.5 Machine learning algorithms to learn complex patterns in miRNA-target interactions
- 4.6 Other useful resources and databases for miRNA studies
- 4.7 Challenges and limitations in computational identification of miRNA targets
- 4.8 Concluding remarks and perspectives
- References
- Chapter 5. Turning data to knowledge: online tools, databases, and resources in microRNA research
- Abstract
- 5.1 Human miRNA regulation
- 5.2 The scope and organization of the chapter
- 5.3 Repositories for miRNA: catalogs and genome browsers
- 5.4 Gateway for miRNAs: integrative platforms
- 5.5 miRNA gene regulation: transcription factors and cellular context
- 5.6 miRNA-targets prediction: experiments and validations
- 5.7 miRNA-target databases: network and pathways
- 5.8 miRNA sponge: ceRNA and lncRNA interaction
- 5.9 Genomic miRNA database: variations and isomiRs
- 5.10 miRNA dysregulation: diseases, cancer, and signaling
- 5.11 Summary and future perspectives
- References
- Part IV: Molecular mechanisms and gene regulation
- Chapter 6. Function of microRNAs in the cytoplasm
- Abstract
- 6.1 Introduction
- 6.2 Subcellular localization of miRNAs
- 6.3 Repression of gene expression via mRNA degradation
- 6.4 Repression of gene expression via translational repression
- 6.5 Enhancement of gene expression
- 6.6 miRNA regulation of gene expression is cell context-specific
- 6.7 Conclusions
- References
- Chapter 7. MicroRNA turnover and nuclear function
- Abstract
- 7.1 Introduction
- 7.2 miRNA biogenesis
- 7.3 Nucleocytoplasmic shuttling of pre-miRNA
- 7.4 Nuclear localization of processing proteins and miRNAs
- 7.5 miRNA functions in the nucleus
- 7.6 miRNA turnover
- 7.7 Conclusions
- References
- Chapter 8. MicroRNA-mediated transcriptional and posttranscriptional regulation
- Abstract
- 8.1 Introduction
- 8.2 Biogenesis of microRNAs
- 8.3 Regulatory function of nuclear microRNA
- 8.4 Regulatory function of mitochondrial microRNA
- 8.5 Transcriptional gene regulation mediated by nuclear microRNAs
- 8.6 microRNAs regulate transcription by interaction with gene promoters
- 8.7 Conclusion
- References
- Chapter 9. Epigenetic regulation and microRNA expression
- Abstract
- 9.1 Introduction
- 9.2 microRNAs
- 9.3 Epigenetic regulation of microRNAs
- 9.4 microRNAs affect epigenetic expression
- 9.5 microRNAs-epigenetic regulator drugs
- 9.6 Conclusion and future prospective
- References
- Chapter 10. RNA m6A modification and microRNAs
- Abstract
- 10.1 Overview of epitranscriptome
- 10.2 m6A and miRNA
- 10.3 Conclusions
- References
- Chapter 11. Unconventional functions of miRNAs
- Abstract
- 11.1 Unconventional subcellular localizations of miRNAs
- 11.2 Unconventional miRNA functions: activation of innate immune sensors
- 11.3 Other unconventional miRNA functions
- Acknowledgments
- Conflict of interest statement
- References
- Part V: MicroRNA as biomarkers
- Chapter 12. Detection methodologies for microRNA biomarker profiling
- Abstract
- 12.1 Introduction
- 12.2 miRNA extraction methods
- 12.3 miRNA profiling methods
- 12.4 Conclusions
- References
- Chapter 13. Endogenous circulating microRNAs in breast milk
- Abstract
- 13.1 Premature newborns have a fragility to protect
- 13.2 Function of the innate immunity
- 13.3 MicroRNAs and regulation of innate immunity
- 13.4 From B3 (breast, bank, bench) to bedside
- 13.5 The routine practice in breast milk banks
- 13.6 The new concept of micro-milk
- 13.7 Stability of microRNAs and extracellular vesicle isolation
- References
- Part VI: MicroRNAs and human diseases
- Chapter 14. MicroRNAs and cancer
- Abstract
- 14.1 Introduction
- 14.2 OncomiRs in breast cancer
- 14.3 Tumor suppressor microRNAs in breast cancer
- 14.4 OncomiRs in lung cancer
- 14.5 Tumor suppressor microRNAs in lung cancer
- 14.6 OncomiRs in hepatocellular carcinoma
- 14.7 Tumor suppressor microRNAs in hepatocellular carcinoma
- 14.8 OncomiRs in pancreatic cancer
- 14.9 Tumor suppressor microRNAs in pancreatic cancer
- 14.10 OncomiRs in colorectal cancer
- 14.11 Tumor suppressor microRNAs in colorectal cancer
- 14.12 OncomiRs in prostate cancer
- 14.13 Tumor suppressor microRNAs in prostate cancer
- 14.14 OncomiRs in gastric cancer
- 14.15 Tumor suppressor microRNAs in gastric cancer
- 14.16 OncomiRs in ovarian cancer
- 14.17 Tumor suppressor microRNAs in ovarian cancer
- 14.18 Conclusion/future directions
- References
- Chapter 15. MicroRNAs and the immune system
- Abstract
- 15.1 Introduction
- 15.2 MicroRNAs and innate immunity
- 15.3 MicroRNAs and pattern recognition receptors
- 15.4 MicroRNAs and induction of IFN signaling pathways
- 15.5 MicroRNAs, IFN receptors, and JAK-STAT pathway
- 15.6 MicroRNAs and IFN-stimulated genes
- 15.7 microRNAs and adaptive immunity
- 15.8 MicroRNA regulation of T lymphocytes immunity
- 15.9 MicroRNAs and T-cell development
- 15.10 MicroRNAs and T-cells activation, proliferation, and differentiation
- 15.11 MicroRNAs and mature regulatory T cell
- 15.12 MicroRNAs and B cells
- 15.13 MicroRNAs involved in the early stages of B-cell development
- 15.14 MicroRNAs involved in the late stages of B-cell development
- 15.15 Conclusions
- References
- Chapter 16. MicroRNAs in cardiovascular diseases
- Abstract
- 16.1 miRNAs in the biology of cardiovascular disease
- 16.2 miRNAs in arrhythmia
- 16.3 miRNA in cardiac hypertrophy
- 16.4 miRNAs in arthrosclerosis
- 16.5 miRNAs in coronary heart disease
- References
- Chapter 17. MicroRNAs in neurological diseases
- Abstract
- 17.1 Introduction
- 17.2 Biogenesis of miRNA
- 17.3 Role of miRNAs in the regulation of neuronal function, development, and survival
- 17.4 miRNAs in the neurodevelopmental and psychiatric disorders
- 17.5 miRNAs in the neurodegenerative disorders
- 17.6 Conclusion and future perspective
- Acknowledgments
- Conflict of interest statement
- References
- Chapter 18. Role of miRNAs in muscle atrophy: the myotonic dystrophy paradigm
- Abstract
- 18.1 General introduction
- 18.2 Role of miRNAs regulating pathways related to muscle atrophy
- 18.3 Role of miRNAs in muscle atrophy during human disease
- 18.4 miRNAs and muscle atrophy in myotonic dystrophy
- 18.5 Concluding remarks and future perspectives
- References
- Chapter 19. MiRNAs in liver fibrosis: new targets and opportunities for therapy
- Abstract
- 19.1 Update of mechanism and target genes
- 19.2 miRNA as potential markers for liver cirrhosis
- 19.3 Development of novel therapy for liver cirrhosis based on miRNA
- 19.4 Role of exosomes as a natural vector of miRNA in the diagnosis and treatment of liver cirrhosis
- 19.5 Conclusion
- Reference
- Chapter 20. MicroRNAs and diabetes mellitus
- Abstract
- 20.1 Introduction to the biology of microRNAs
- 20.2 Definitions of type 1 and 2 diabetes mellitus
- 20.3 Circulating miRNAs as biomarkers of diabetes mellitus
- 20.4 miRNAs and β-cell function
- 20.5 miRNAs and gluconeogenesis
- 20.6 Exosomal miRNAs and diabetes mellitus
- 20.7 miRNAs in α-cells
- 20.8 miRNAs and insulin resistance
- 20.9 miRNAs and adipose tissue in diabetes mellitus
- 20.10 miRNAs and diabetic nephropathy
- 20.11 miRNAs and diabetic retinopathy
- 20.12 miRNAs and diabetic neuropathy
- References
- Chapter 21. MicroRNA control of kidney disease
- Abstract
- 21.1 Kidney structure and function
- 21.2 MicroRNA biogenesis and function
- 21.3 MicroRNAs in renal physiology
- 21.4 MicroRNA dysregulation in renal diseases
- 21.5 Clinical applications, therapeutic strategies, and perspectives
- Acknowledgments
- References
- Chapter 22. MicroRNA in leukemia
- Abstract
- 22.1 Introduction
- 22.2 MicroRNA deregulation in chronic myeloid leukemia
- 22.3 Conclusions
- References
- Chapter 23. MicroRNAs and other noncoding RNAs in human pathology
- Abstract
- 23.1 Introduction
- 23.2 Relationship between miRNAs and other ncRNAs in human pathology
- 23.3 Conclusion
- References
- Part VII: MicroRNA based therapeutics
- Chapter 24. Overview of microRNA-based therapeutics
- Abstract
- 24.1 MicroRNA mimic therapeutics
- 24.2 Antisense oligonucleotides anti-microRNAs therapeutics
- 24.3 Pharmacokinetic of antisense oligonucleotides anti-microRNAs
- 24.4 Delivery systems of microRNA therapeutics
- 24.5 Preclinical validation and ongoing clinical trials of microRNA therapeutics
- 24.6 Drug resistance and microRNA therapeutics: a novel therapeutic paradigm
- References
- Chapter 25. MicroRNA-based therapeutic strategies for cancer
- Abstract
- 25.1 Introduction
- 25.2 RNA modifications
- 25.3 Delivery systems
- 25.4 MiRNA-based therapies in specific cancer types
- 25.5 Conclusion and future perspectives
- References
- Chapter 26. MicroRNA targeted therapy in cardiovascular disease
- Abstract
- 26.1 Introduction
- 26.2 Conclusion
- References
- Chapter 27. MicroRNA targeted therapy in hepatitis
- Abstract
- 27.1 Hepatitis
- 27.2 miRNA
- 27.3 miRNAs in hepatitis
- 27.4 MicroRNA targeted therapy in hepatitis
- 27.5 Perspective and future directions
- References
- Chapter 28. MicroRNA-based therapeutic strategies for chronic kidney disease and uremic cardiomyopathy
- Abstract
- 28.1 Introduction
- 28.2 Etiology and pathomechanisms of kidney and heart injury in chronic kidney disease
- 28.3 Biogenesis and function of miRs
- 28.4 miRs as therapeutic tools
- 28.5 miR expressional changes in chronic kidney disease and uremic cardiomyopathy
- 28.6 Shared miRs in chronic kidney disease and uremic cardiomyopathy
- 28.7 Conclusions and future perspectives
- Acknowledgments
- References
- Chapter 29. Clinical applications of microRNAs
- Abstract
- 29.1 Introduction
- 29.2 Key concepts of miRNA biology
- 29.3 miRNAs in clinical applications
- 29.4 Concluding remarks and future directions
- References
- Part VIII: Concluding perspectives
- Chapter 30. Prospective advances in microRNAs investigation
- Abstract
- 30.1 Introduction
- 30.2 Biogenesis and biological functions of miRNAs
- 30.3 MiRNAs in the extracellular vesicles: a new hot spot in miRNA research
- 30.4 Perspective prospect of clinical application of miRNAs
- 30.5 Conclusions and perspectives
- Acknowledgments
- References
- Index
- Edition: 1
- Published: July 19, 2022
- No. of pages (Paperback): 654
- No. of pages (eBook): 654
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9780323897747
- eBook ISBN: 9780323885584
JX
Junjie Xiao
Dr. Junjie Xiao is Professor and Vice Dean of School of Life Science, and also the Vice Dean of School of Medicine, at Shanghai University, People's Republic of China. Dr. Xiao is the Associate Editor of the Journal of Cardiovascular Translational Research, BMC Sports and Science, Medicine and Rehabilitation, and Frontiers in Cardiovascular Medicine. He is also the member of the editorial board of BMC Medicine, Journal of Molecular and Cellular Cardiology, Journal of Sport and Health Science, Cell Transplantation, Current Stem Cell Research & Therapy, Journal of Thoracic Disease, and Biomedical and Environmental Sciences. He is the author or co-author of 140 scientific articles in various journals and his major research interest is studying the comprehensive intervention and risk warning of heart failure, especially using exercise as a platform to identify novel targets, including non-coding RNAs and functional proteins, for enhancing cardiac regeneration and combating heart failure.
Affiliations and expertise
Professor, School of Life Science, Shanghai University, Shanghai, People's Republic of China; Vice Dean, School of Life Science, Shanghai University, Shanghai, People's Republic of ChinaRead MicroRNA on ScienceDirect