Microfluidics-Aided Technologies

Platforms for Next Generation Biological Applications

1st Edition - November 23, 2024
Imprint: Academic Press
Editors: Dhananjay Bodas, Virendra Gajbhiye
Language: English
Paperback ISBN:
9 7 8 - 0 - 3 2 3 - 9 5 5 3 3 - 1
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 9 5 5 3 4 - 8

Microfluidics-Aided Technologies: Platforms for Next Generation Biological Applications aims to provide comprehensive information of microfluidic technologies, their developme… Read more

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Microfluidics-Aided Technologies: Platforms for Next Generation Biological Applications aims to provide comprehensive information of microfluidic technologies, their development and biomedical applications. The book opens with an overview of the fundamentals of microfluidics. It is followed by discussions of methods and biomedical applications of microfluidics in diagnostics, prognostics, cell engineering, gene sequencing, cellular analysis, and bioimaging. The book also explores breakthroughs in microfluidics such as 3D bioprinting, organ-on-chip technologies, and regenerative medicine.

This book offers researchers an interdisciplinary perspective towards biological problems. It is a resource for advanced undergraduate, graduate students, researchers and industry scientists interested in the emergence of advance techniques and next generation microfluidics-aided technologies for applications in the biomedical and medical research.

Title of Book
Cover image
Title page
Table of Contents
Copyright
Contributors
Chapter 1. Fundamentals of microfluidics
1 Introduction to microfluidics
2 Size benefits
3 Automation and integration
4 Flows in microfluidics
5 Pressure driven flows
6 Diffusion in microfluidics
7 Electrokinetic forces in microfluidics
8 Prerequisites of microfluidics fabrication and analysis
8.1 Materials selection
8.2 Glass and silicon
8.3 Elastomers
8.4 Thermoplastics
8.5 Hydrogels
8.6 Paper-based materials
8.7 Fabrication methods
8.8 Photolithography
8.9 Soft lithography
8.10 Hot embossing and injection molding
8.11 Hot embossing
8.12 Laser ablation and micromachining
8.13 Direct writing
8.14 Polymer casting
9 Types of microfluidic systems
9.1 Continuous-flow microfluidics
9.1.1 Principles and components
9.1.2 Applications
9.1.3 Advantages
9.2 Digital microfluidics
9.2.1 Principles and components
9.2.2 Applications
9.2.3 Advantages
9.3 Paper-based microfluidics
9.3.1 Principles and architecture
9.3.2 Applications
9.3.3 Advantages
9.4 Organ-on-a-chip
9.4.1 Principles and design
9.4.2 Applications
9.4.3 Examples of organ-on-a-chip systems
9.4.4 Challenges and future directions
9.5 Centrifugal microfluidics
9.5.1 Principles and design
9.5.2 Applications
9.5.3 Advantages
9.5.4 Challenges and future directions
10 Conclusion
Chapter 2. Microfluidic systems in diagnostic and prognostic applications
1 Introduction
2 Key advantages of microfluidic devices
3 Enzyme-linked immunosorbent assays
3.1 ELISA on flexible microfluidic systems
3.2 Droplets-based ELISA
3.3 Paper-based ELISA (p-ELISA)
4 Molecular diagnostics
4.1 Digital droplet PCR
4.2 LSPR-integrated on-chip DNA diagnostics
4.3 Molecular diagnostics on paper-based devices
5 Example I: COVID-19 diagnostics
6 Example II: Sepsis diagnosis
7 Hydrodynamic cell separation techniques
8 Bead-based separation techniques
9 Other techniques
10 CTC detection
11 Hydrodynamic approches
12 Other approaches
13 Microfluidics-based prognostic development
14 Conclusions
Chapter 3. Microfluidics in drug screening and drug delivery
1 Introduction
2 Microfluidics in drug screening
2.1 Droplet microfluidics
2.2 Organ-on-chip
2.3 3D cell culture
2.4 Slip-driven microfluidic devices
2.5 Other microfluidic chips for drug screening
3 Microfluidics in drug delivery
4 Conclusion
Chapter 4. Microfluidics assisted cell engineering and manipulation
1 Introduction
1.1 Magnetic manipulation
1.2 Electric manipulation
1.3 Optical manipulation
1.4 Acoustic manipulation
1.5 Sedimentation-based cell engineering and manipulation
1.6 Biomarker-based cell engineering and manipulation
1.7 Microarray-based cell engineering and manipulation
1.7.1 DNA microarray
1.7.2 Protein microarray
1.7.3 Cell-based microarray
2 Conclusion
Chapter 5. Microfluidic-assisted cell analysis: Molecular assay and biochemical assay
1 Introduction
2 Microfluidic-assisted cell analysis
2.1 Single-cell isolation and analysis
2.1.1 Droplet-based systems
2.1.2 Valve-based systems
2.1.3 Microwell-based systems
2.1.4 Employing microposts
2.2 Cell lysis
2.2.1 Chemical methods for lysis
2.2.2 Mechanical methods for lysis
2.2.3 Electroporation-based methods for lysis
2.2.4 Laser-based lysis
2.2.5 Thermal methods for lysis
2.3 DNA extraction and amplification
2.3.1 Serpentine design
2.3.2 The oscillating-flow design
2.3.3 Centrifugal microfluidic devices
2.3.4 Lab disk
2.3.5 Array-based assays
2.4 Biomarker detection
2.4.1 Chip-based microfluidics
2.4.2 Paper-based microfluidics
2.5 Cell separation
2.5.1 Differentiation using fluorescence and imaging techniques
2.5.2 Magnetic separation
2.5.3 Mechanical and hydrodynamic separation
3 Conclusion
Chapter 6. Microfluidics in bioimaging: In vitro and in vivo advancements
1 Introduction
2 Microfluidics for in vitro bioimaging
2.1 Microfluidic devices
2.2 Microfluidic devices for cell imaging
2.3 Microfluidic devices for cancer imaging
2.4 Microfluidic devices for imaging cell dynamics
3 In vivo bioimaging using microfluidic devices
3.1 Microfluidic devices for in vivo bioimaging of small organisms
3.2 Microfluidics for in vivo organ on chip imaging
4 Conclusion and future perspective
Chapter 7. Genetic sequencing and editing using microfluidics: System on chip approach
1 Introduction
2 Genetic sequencing and gene editing overview
3 Microfluidics and genetics
3.1 DNA analysis using microfluidics
3.2 Gene expression analysis using microfluidics
3.3 DNA mutation detection using microfluidics
4 Gene editing via microfluidics
5 Future perspective and conclusion
Chapter 8. Anaphylactic detection using microfluidic systems
1 Introduction
2 Types of anaphylaxis
2.1 IgE-mediated anaphylaxis
2.2 IgE-nonmediated anaphylaxis
3 Methods of anaphylactic detection
3.1 Immunoassay
3.1.1 ELISA
3.1.2 Lateral flow immunoassay
3.1.3 xMAP
3.2 Biosensor
3.2.1 Optical biosensor
3.2.2 Electrochemical biosensor
4 Microfluidics-based detection methods
4.1 Microfluidics in immunoassay
4.1.1 Microfluidic-based ELISA
4.1.2 Microfluidics in paper-based immunoassay
4.2 Microfluidics-based on-chip analysis of nucleic acid
4.2.1 Microfluidics in on-chip PCR
4.2.2 Microfluidics in on-chip isothermal nucleic acid amplification
4.3 Microfluidics-based aptasensor
4.4 Microfluidics-based electrochemical detection
5 Challenges and trends to overcome
6 Conclusion
Chapter 9. Microfluidic systems for bacterial and fungal research
1 Introduction
2 Emergence of microfluidic systems
2.1 Importance of microfluidic systems in biological research
2.2 The bacterial and fungal research
2.3 Purpose and scope of the chapter
3 Fundamentals of microfluidic systems
3.1 Definition of microfluidics
3.2 Principles of microfluidics
3.3 Key components and design considerations in microfluidic systems
3.4 Microchannels and substrate materials
3.5 Microvalves and micropumps
3.6 Mixers and separators
3.7 Integration with detection systems
3.8 Design considerations
3.9 Advantages of microfluidics
3.10 Limitations of microfluidics
4 Applications of microfluidics in bacterial research
4.1 Microfluidic platforms for bacterial culture and growth
4.2 Exploring bacterial behavior and interactions with microfluidics
4.3 Synergy between bacterial studies and microfluidics advancements
5 Applications of microfluidics in fungal research
5.1 Notable research findings
5.2 Microfluidic devices for fungal cultivation and observation
5.3 Microfluidic devices for fungal cultivation
5.4 Fungal pathogenesis studies in microfluidic systems
5.5 Fungal biotechnology and microfluidics: A symbiosis for innovation
6 Integration of microfluidic systems with analytical techniques
6.1 Combining microfluidics with imaging and sensing
6.1.1 Microfluidics and imaging
6.1.2 Microfluidics and sensing
6.1.3 Applications and advantages
6.2 Data collection and analysis in microfluidic experiments
6.2.1 Process and methodology
6.2.2 Origin and early use
6.2.3 Research aspects
6.2.4 Basic rules and limitations
6.3 Emerging trends in integration of microfluidic systems with analytical techniques
6.3.1 Successes
6.3.2 Diversities and specifications
6.3.3 Proposed research
7 Challenges and future directions
Chapter 10. Importance of microfluidics in cancer modeling
1 Introduction
2 What is cancer
3 Role of TME in cancer progression
4 In vitro disease modeling
5 Tumor-on-chip as a cancer model
5.1 Tumor spheroids
5.2 Tumor organoids
6 Conclusion
Chapter 11. Microfluidics in bioanalytical chemistry
1 Introduction
2 Applications of microfluidics
2.1 DNA assays
2.2 Immunoassays
2.3 Cell-based assays
2.4 Cancer
2.5 Diagnosis and imaging
2.6 Drug delivery
3 Microfluidics advances for COVID-19
4 Conclusion
Chapter 12. Role of microfluidics in 3D bioprinting
1 Introduction
1.1 3D-printed microfluidics
1.2 Microfluidics-enabled 3D printing
2 Handheld microfluidic devices
3 Conclusion
Chapter 13. Tissue-on-chip, organ-on-chip, and organism-on-chip
1 Introduction
2 Design and fabrication of OoCs
2.1 Geometry
2.2 Fabrication materials
2.3 Fabrication methods
3 Sterilization of OoC platforms
4 Applications of OoC platforms
4.1 Lung
4.2 Liver
4.3 Brain
4.4 Heart
4.5 Other OoC platforms
5 Challenges, opportunities, and future directions
6 Conclusion
Chapter 14. Microfluidics in regenerative medicine
1 Introduction
2 Regenerative medicine overview
2.1 Tissue engineering and regenerative medicine
2.2 Stem cells and regenerative medicine
3 Stem cells in microfluidic systems
4 Tissue regeneration and microfluidics
4.1 Wound healing
4.2 Heart regeneration
4.3 Liver regeneration
4.4 Neuronal regeneration
4.5 Bone regeneration
5 Future perspective and conclusion
Chapter 15. Microfluidics in protein engineering
1 Introduction
2 Simple standard test tubes-based batch reactions
3 CFPS combined with synthetic cells
4 Microfluidics at the CFPS/artificial cells interface
5 CFPS applications in biotechnology and synthetic biology
5.1 Solid surfaces
5.1.1 Droplets-based CFPS
5.1.2 DNA hydrogels
5.2 Microfluidics-based microgel
5.3 DNA brush
5.4 Membrane compartments: Vesicular particles
5.4.1 Liposomes
5.4.2 Polymersome
5.4.3 Proteinososme
5.4.4 Nano disc
6 Conclusion
Index

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Microfluidics-Aided Technologies

Platforms for Next Generation Biological Applications

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Dhananjay Bodas

Virendra Gajbhiye

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