Methods of Hormone Radioimmunoassay

Methods of Hormone Radioimmunoassay, Second Edition is a compilation of radioimmunoassay techniques for the quantification of hormones. Various kinds of hormones are considered, namely: intracellular messengers; hypothalamic and pineal hormones; pituitary hormones; thyroid and parathyroid hormones; renal hormones; hormones of the gastrointestinal tract; pancreatic hormones; steroid hormones; uterine and placental hormones; vasoactive peptide hormones; and growth factors. The corresponding method of radioimmunoassay for each hormone is described. This book is comprised of 49 chapters and begins with a discussion on the radioimmunoassay of cyclic adenosine-3',5-monophosphate (cAMP) and cyclic guanosine-3,5'-monophosphate (cGMP), together with problems related to measuring both compounds. The reader is then introduced to prostaglandins and prostaglandin metabolites, thyrotropin-releasing hormone, and gonadotropin-releasing hormone. Pituitaty gonadotropins, prolactin, and thyrotropin are also discussed. The remaining chapters deal with hormones of the gastrointestinal tract, pancreatic hormones, steroid hormones, and growth factors. This monograph is intended for both clinicians and investigators interested in methods of hormone radioimmunoassay.

List of Contributors

Preface

Preface to First Edition

Intracellular Messengers

1 Cyclic AMP and Cyclic GMP

I. Introduction

II. Method of Radioimmunoassay

III. Problems Related to Measuring cAMP and cGMP

References

2 Prostaglandins and Prostaglandin Metabolites

I. Introduction

II. Method of Radioimmunoassay

III. Evaluation of the Radioimmunoassay

IV. Prostaglandin Metabolites

References

Hypothalamic and Pineal Hormones

3 Thyrotropin-Releasing Hormone

I. Introduction

II. Method of Radioimmunoassay

III. Assay and Inactivation of TRH in Biologic Fluids and Tissue

IV. Conclusions

References

4 Gonadotropin-Releasing Hormone

I. Introduction

II. Radioimmunoassay

III. Summary

References

5 Somatostatin

I. Introduction

II. Materials

III. Methods

IV. Characteristics of the SRIF Radioimmunoassay

V. Measurement of SRIF

References

6 Melatonin

I. Introduction

II. Methods of Radioimmunoassay

III. Radioimmunoassay Technique

IV. Applications and Importance of Melatonin Radioimmunoassay

V. Radioiodinated Melatonin Analog

References

7 Substance P

I. Introduction

II. Assay Techniques

III. Some Characteristics and Results of the Assay

References

8 Neurotensin and Related Substances

I. Introduction

II. General Considerations for Intermediate-Sized Peptides

III. Specific Considerations for Neurotensin

IV. Methods

V. Conclusion

References

Pituitary Hormones

9 Pituitary Gonadotropins

I. Introduction

II. Procedural Details of Radioimmunoassay

III. Application to Measurement of Tissue Receptor-Bound LH

IV Normal Values for Human LH and FSH

V. Conclusions

References

10 Prolactin

I. Introduction

II. Bioassays

III. Radioimmunoassays

IV. Radioimmunoassay Method

V. Hormonal Heterogeneity

VI. Clinical Results

References

11 Growth Hormone

I. Introduction

II. Preparation of Buffers and Columns for hGH Assay

III. Technique of Radioimmunoassay

IV. Cross-Reactivity with Other Hormones

V. Measurements of the Hormone in Blood

VI. Measurements of the Hormone in Tissue and Urine

References

12 Adrenocorticotropic Hormone (ACTH)

I. Introduction

II. Methods of Radioimmunoassay

III. Problems

IV. Other Radioimmunoassays

V Recent Developments

References

13 Melanocyte-Stimulating Hormones (MSH's) and Lipotropic Hormones (LPH's)

I. Introduction

II. Methods of Radioimmunoassay

III. Problems

References

14 Thyrotropin

I. Introduction

II. Method of Radioimmunoassay

III. Other Immunoassays

References

15 Oxytocin

I. Introduction and History

II. Methods of Radioimmunoassay

III. Sensitivity, Cross-Reactivity, and Validation

IV Oxytocin in Plasma

V. Discussion

References

16 Vasopressin

I. Introduction and History

II. Methods of Radioimmunoassay

III. Sensitivity, Cross-Reactivity, and Validation

IV. Vasopressin in Plasma

V. Comparison with Other Assays

References

Thyroid and Parathyroid Hormones

17 Human Calcitonin: Application of Affinity Chromatography

I. Introduction

II. Method of Radioimmunoassay

III. Interpretation of Radioimmunoassay Data

IV. Concentrations of Calcitonin in Human Serum and Urine

V. Affinity Chromatography

References

18 Thyroxine and Triiodothyronine

I. Introduction

II. Principles of T3 and T4 Radioimmunoassay

III. Rapid Simultaneous Radioimmunoassay for T3 and T4 in Unextracted Serum

IV. Comparison of Various T3 and T4 Radioimmunoassay Techniques and Results

V. Physiological Considerations

VI. Screening for Congenital Hypothyroidism

VII. Summary and Conclusions

References

19 Parathyroid Hormone

I. Introduction

II. Methodology

III. Interpretation of Radioimmunoassay Data

References

Renal Hormones

20 Erythropoietin

I. Introduction

II. Erythropoietin Sources

III. Radioimmunoassay

IV. Measurements of Erythropoietin in Blood

V Final Comments

References

21 Vitamin D Metabolites

I. Introduction

II. 25-OHD Assays

III. 1,25-(OH)2D Assays

IV. 24,25-(OH)2D Assays

V. Summary

References

Addendum

Hormones of the Gastrointestinal Tract

22 Gastrin and Related Peptides

I. Introduction

II. Methods of Radioimmunoassay

III. Evaluation of Radioimmunoassay Data

IV. Measurement of Gastrin Tetrapeptide

References

23 Secretin

I. Introduction

II. Methods of Radioimmunoassay

III. Evaluation and Validation of the Method

References

24 Cholecystokinin-Pancreozymin

I. Introduction

II. Methods

III. Evaluation of the Radioimmunoassay

IV. Results Obtained with Cholecystokinin Radioimmunoassays

References

25 Serotonin

I. Introduction

II. Methods of Radioimmunoassay

III. Validation and Characterization of the Assay

IV. Summary

References

26 Gastric Inhibitory Polypeptide

I. Introduction

II. Method of Radioimmunoassay

III. Evaluation of Radioimmunoassay Data

References

27 Vasoactive Intestinal Peptide

I. Introduction

II. Method of Radioimmunoassay

III. Assay Characteristics

IV. Conclusion

References

28 Motilin

I. Introduction

II. Method of Radioimmunoassay

III. Evaluation of the Radioimmunoassay

IV Affinity Chromatography

References

29 Bombesin-like Peptides

I. Introduction

II. Method of Radioimmunoassay

III. Bombesin-like Material in Tissues

References

30 Bile Acids

I. Introduction

II. Methods of Radioimmunoassay

III. Vilidation of Assay

IV Normal Values and Concentrations of the Individual Bile Acids in Serum in Various States of Hepatobiliary Disease

References

Pancreatic Hormones

31 Insulin, Proinsulin, and C-Peptide

I. Introduction

II. Methods of Radioimmunoassay

III. Verification of Assay Data

IV Problems Related to the Measurement of Insulin and Proinsulin

V. Other Radioimmunoassays Available

VI. Radioimmunoassay for Human C-Peptide

References

32 Glucagon

I. Introduction

II. Special Problems Encountered in the Measurement of Plasma Glucagon by Radioimmunoassay

III. Methods of Radioimmunoassay

References

33 Human Pancreatic Polypeptide (HPP) and Bovine Pancreatic Polypeptide (BPP)

I. Introduction

II. Methods of Radioimmunoassay

III. Homologous and Heterologous Assays

IV. Alternate Methods

References

Steroid Hormones

34 Plasma Estradiol, Estrone, Estriol, and Urinary Estriol Glucuronide

I. Plasma Estradiol, Estrone, and Estriol

II. Urinary Estriol Glucuronide

References

35 Progesterone and 20a-Dihydroprogesterone

I. Introduction

II. Methods of Radioimmunoassay

III. Sensitivity and Precision

References

36 Androgens: Testosterone and Dihydrotestosterone

I. Introduction

II. Method of Radioimmunoassay

III. Validation of Assay

IV. Normal Values

V. Other Androgens—Ahdrostenedione

References

37 Radioiodinated Steroid Hormones—General Principles

I. Introduction

II. Advantages and Disadvantages of Radioiodinated Steroids

III. Preparation of the Steroid Analogs for Radioiodination

IV. Radioiodination of Steroid Hormones

V. Purification of Radioiodinated Steroid Hormones

VI. Development and/or Adaptation of Radioimmunoassay Procedures

VII. Summary

References

38 Mineralocorticoids: Aldosterone, Deoxycorticosterone, 18-Hydroxydeoxycorticosterone, and 18-Hydroxycorticosterone

I. Introduction

II. Methods of Radioimmunoassay

III. Evaluation of the Radioimmunoassay

IV. Problems Related to Measuring Mineralocorticoids

V. Other Radioimmunoassay Methods

VI. New Developments

References

39 Glucocorticoids: Cortisol, Cortisone, Corticosterone, Compound S, and Their Metabolites

I. Introduction

II. Methods of Radioimmunoassay

III. Evaluation of Radioimmunoassay Data

IV. Other Uses for Cortisol and Corticosterone Antibody

V. General Discussion

References

40 Arthropod Molting Hormones

I. Introduction

II. Quantification of Ecdysteroids

III. Comparison of Available Radioimmunoassays for Ecdysteroids

IV. Protocol

V. Dual Antisera Determinations

References

Uterine and Placental Hormones

41 Specific Human Chorionic Gonadotropin Assay

I. Introduction

II. Method of Radioimmunoassay

References

42 Radioimmuno- and Radioreceptor Assays of Placental Lactogens

I. Introduction

II. Radioimmunoassay

III. Radioreceptor Assays for hPL

IV. Applications of Radioimmunoassay and Radioreceptor Assays

V. Summary

References

43 Radioimmunoassay of Human Chorionic Thyrotropin

I. Introduction

II. Technique of hCT Radioimmunoassay

III. Results and Interpretation

References

44 Relaxin

I. Introduction

II. Method of Radioimmunoassay

III. Radioimmunoassay Characterization

IV. Levels of Circulating Relaxin

References

Vasoactive Peptide Hormones

45 Renin and the Angiotensins

I. Introduction

II. Methods of Radioimmunoassay

III. Verification of Assay Results and Special Problems

IV. Commercial Kits and Concluding Comments

References

46 Bradykinin

I. Introduction

II. Radioimmunoassay Methodology

III. Validation and Standards

References

47 Urogastrone—Epidermal Growth Factor

I. Introduction

II. Methods of Radioimmunoassay

III. Urogastrone Measurements

References

48 Nerve Growth Factor: Radioimmunoassay and Bacteriophage Immunoassay

I. Background Information

II. Radioimmunoassay

III. Bacteriophage Immunoassay

IV. Measurement of NGF in Cells and Biologic Fluids

V. Summary

References

49 Somatomedin A, Somatomedin C, and NSILA-s

I. Introduction

II. Preparation of Buffers and Columns for the Somatomedins A and C Assays

III. Preparation of Placental Membranes

IV. Technique of Radioreceptor Assay for Somatomedins A and C

V. Preparation of Buffer and Column for the NSILA-s Assay

VI. Technique of Radioreceptor Assay for NSILA-s

VII. Cross-Reactivity with Other Growth Factors

VIII. Measurement of Somatomedins A and C and NSILA-s in Blood

References

Appendixes

I. Summary of Radioimmunoassay Methodology

II. Commercial Sources of Radioimmunoassay Components

III. Normal Values As Determined by Radioimmunoassay

Index

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Methods of Hormone Radioimmunoassay

Purchase options

BLOOM INTO SPRING SAVINGS

Institutional subscription on ScienceDirect

Related books