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Methods of Hormone Radioimmunoassay
2nd Edition - January 28, 1979
Editor: Bernard Jaffe
eBook ISBN:9780323161268
9 7 8 - 0 - 3 2 3 - 1 6 1 2 6 - 8
Methods of Hormone Radioimmunoassay, Second Edition is a compilation of radioimmunoassay techniques for the quantification of hormones. Various kinds of hormones are considered,… Read more
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Methods of Hormone Radioimmunoassay, Second Edition is a compilation of radioimmunoassay techniques for the quantification of hormones. Various kinds of hormones are considered, namely: intracellular messengers; hypothalamic and pineal hormones; pituitary hormones; thyroid and parathyroid hormones; renal hormones; hormones of the gastrointestinal tract; pancreatic hormones; steroid hormones; uterine and placental hormones; vasoactive peptide hormones; and growth factors. The corresponding method of radioimmunoassay for each hormone is described. This book is comprised of 49 chapters and begins with a discussion on the radioimmunoassay of cyclic adenosine-3',5-monophosphate (cAMP) and cyclic guanosine-3,5'-monophosphate (cGMP), together with problems related to measuring both compounds. The reader is then introduced to prostaglandins and prostaglandin metabolites, thyrotropin-releasing hormone, and gonadotropin-releasing hormone. Pituitaty gonadotropins, prolactin, and thyrotropin are also discussed. The remaining chapters deal with hormones of the gastrointestinal tract, pancreatic hormones, steroid hormones, and growth factors. This monograph is intended for both clinicians and investigators interested in methods of hormone radioimmunoassay.
List of Contributors
Preface
Preface to First Edition
Intracellular Messengers
1 Cyclic AMP and Cyclic GMP
I. Introduction
II. Method of Radioimmunoassay
III. Problems Related to Measuring cAMP and cGMP
References
2 Prostaglandins and Prostaglandin Metabolites
I. Introduction
II. Method of Radioimmunoassay
III. Evaluation of the Radioimmunoassay
IV. Prostaglandin Metabolites
References
Hypothalamic and Pineal Hormones
3 Thyrotropin-Releasing Hormone
I. Introduction
II. Method of Radioimmunoassay
III. Assay and Inactivation of TRH in Biologic Fluids and Tissue
IV. Conclusions
References
4 Gonadotropin-Releasing Hormone
I. Introduction
II. Radioimmunoassay
III. Summary
References
5 Somatostatin
I. Introduction
II. Materials
III. Methods
IV. Characteristics of the SRIF Radioimmunoassay
V. Measurement of SRIF
References
6 Melatonin
I. Introduction
II. Methods of Radioimmunoassay
III. Radioimmunoassay Technique
IV. Applications and Importance of Melatonin Radioimmunoassay
V. Radioiodinated Melatonin Analog
References
7 Substance P
I. Introduction
II. Assay Techniques
III. Some Characteristics and Results of the Assay
References
8 Neurotensin and Related Substances
I. Introduction
II. General Considerations for Intermediate-Sized Peptides
III. Specific Considerations for Neurotensin
IV. Methods
V. Conclusion
References
Pituitary Hormones
9 Pituitary Gonadotropins
I. Introduction
II. Procedural Details of Radioimmunoassay
III. Application to Measurement of Tissue Receptor-Bound LH
IV Normal Values for Human LH and FSH
V. Conclusions
References
10 Prolactin
I. Introduction
II. Bioassays
III. Radioimmunoassays
IV. Radioimmunoassay Method
V. Hormonal Heterogeneity
VI. Clinical Results
References
11 Growth Hormone
I. Introduction
II. Preparation of Buffers and Columns for hGH Assay
III. Technique of Radioimmunoassay
IV. Cross-Reactivity with Other Hormones
V. Measurements of the Hormone in Blood
VI. Measurements of the Hormone in Tissue and Urine
References
12 Adrenocorticotropic Hormone (ACTH)
I. Introduction
II. Methods of Radioimmunoassay
III. Problems
IV. Other Radioimmunoassays
V Recent Developments
References
13 Melanocyte-Stimulating Hormones (MSH's) and Lipotropic Hormones (LPH's)
I. Introduction
II. Methods of Radioimmunoassay
III. Problems
References
14 Thyrotropin
I. Introduction
II. Method of Radioimmunoassay
III. Other Immunoassays
References
15 Oxytocin
I. Introduction and History
II. Methods of Radioimmunoassay
III. Sensitivity, Cross-Reactivity, and Validation
IV Oxytocin in Plasma
V. Discussion
References
16 Vasopressin
I. Introduction and History
II. Methods of Radioimmunoassay
III. Sensitivity, Cross-Reactivity, and Validation
IV. Vasopressin in Plasma
V. Comparison with Other Assays
References
Thyroid and Parathyroid Hormones
17 Human Calcitonin: Application of Affinity Chromatography
I. Introduction
II. Method of Radioimmunoassay
III. Interpretation of Radioimmunoassay Data
IV. Concentrations of Calcitonin in Human Serum and Urine
V. Affinity Chromatography
References
18 Thyroxine and Triiodothyronine
I. Introduction
II. Principles of T3 and T4 Radioimmunoassay
III. Rapid Simultaneous Radioimmunoassay for T3 and T4 in Unextracted Serum
IV. Comparison of Various T3 and T4 Radioimmunoassay Techniques and Results
V. Physiological Considerations
VI. Screening for Congenital Hypothyroidism
VII. Summary and Conclusions
References
19 Parathyroid Hormone
I. Introduction
II. Methodology
III. Interpretation of Radioimmunoassay Data
References
Renal Hormones
20 Erythropoietin
I. Introduction
II. Erythropoietin Sources
III. Radioimmunoassay
IV. Measurements of Erythropoietin in Blood
V Final Comments
References
21 Vitamin D Metabolites
I. Introduction
II. 25-OHD Assays
III. 1,25-(OH)2D Assays
IV. 24,25-(OH)2D Assays
V. Summary
References
Addendum
Hormones of the Gastrointestinal Tract
22 Gastrin and Related Peptides
I. Introduction
II. Methods of Radioimmunoassay
III. Evaluation of Radioimmunoassay Data
IV. Measurement of Gastrin Tetrapeptide
References
23 Secretin
I. Introduction
II. Methods of Radioimmunoassay
III. Evaluation and Validation of the Method
References
24 Cholecystokinin-Pancreozymin
I. Introduction
II. Methods
III. Evaluation of the Radioimmunoassay
IV. Results Obtained with Cholecystokinin Radioimmunoassays
References
25 Serotonin
I. Introduction
II. Methods of Radioimmunoassay
III. Validation and Characterization of the Assay
IV. Summary
References
26 Gastric Inhibitory Polypeptide
I. Introduction
II. Method of Radioimmunoassay
III. Evaluation of Radioimmunoassay Data
References
27 Vasoactive Intestinal Peptide
I. Introduction
II. Method of Radioimmunoassay
III. Assay Characteristics
IV. Conclusion
References
28 Motilin
I. Introduction
II. Method of Radioimmunoassay
III. Evaluation of the Radioimmunoassay
IV Affinity Chromatography
References
29 Bombesin-like Peptides
I. Introduction
II. Method of Radioimmunoassay
III. Bombesin-like Material in Tissues
References
30 Bile Acids
I. Introduction
II. Methods of Radioimmunoassay
III. Vilidation of Assay
IV Normal Values and Concentrations of the Individual Bile Acids in Serum in Various States of Hepatobiliary Disease
References
Pancreatic Hormones
31 Insulin, Proinsulin, and C-Peptide
I. Introduction
II. Methods of Radioimmunoassay
III. Verification of Assay Data
IV Problems Related to the Measurement of Insulin and Proinsulin
V. Other Radioimmunoassays Available
VI. Radioimmunoassay for Human C-Peptide
References
32 Glucagon
I. Introduction
II. Special Problems Encountered in the Measurement of Plasma Glucagon by Radioimmunoassay
III. Methods of Radioimmunoassay
References
33 Human Pancreatic Polypeptide (HPP) and Bovine Pancreatic Polypeptide (BPP)
I. Introduction
II. Methods of Radioimmunoassay
III. Homologous and Heterologous Assays
IV. Alternate Methods
References
Steroid Hormones
34 Plasma Estradiol, Estrone, Estriol, and Urinary Estriol Glucuronide
I. Plasma Estradiol, Estrone, and Estriol
II. Urinary Estriol Glucuronide
References
35 Progesterone and 20a-Dihydroprogesterone
I. Introduction
II. Methods of Radioimmunoassay
III. Sensitivity and Precision
References
36 Androgens: Testosterone and Dihydrotestosterone