Methods of Enzymatic Analysis

ForewordPrefaceContributorsAbbreviationsSection A: General I. Principles of Enzymatic Analysis II. Experimental Techniques III. Cell and Tissue DisintegrationSection B: Estimation of Substrates Preliminary Remarks I. Substances Involved in Carbohydrate Metabolism 1. Poly-, Oligo- and Disaccharides 2. Monosaccharides and Derivatives 3. Three-Carbon Compounds 4. Two- and One-Carbon Compounds II. Substances Involved in the Citric Acid Cycle a) Citrate b) Citrate and Isocitrate c) α-Oxoglutarate d) L-Malate e) Oxaloacetate f) Succinate g) Fumarate III. Substances Involved in Protein Metabolism 1. Proteins and Peptides 2. Amino Acids, Amines and Amides IV. Substances Involved in the Metabolism of Fatty Acids, Lipids and Steroids a) Polyunsaturated Fatty Acids b) Lecithin c) Acyl-CoA Derivatives d) Acetoacetate e) D-(—)-ß-Hydroxybutyrate f) Hydrolysis of Steroid Conjugates g) 20-Ketosteroids h) Steroid Alcohols in Urine V. Nucleosides, Purines and Pyrimidines, Coenzymes and Related Substances 1. Nucleosides, Purines and Pyrimidines 2. Coenzymes and Related Substances VI. Other Substrates and Miscellaneous Substances a) Organophosphorus Insecticides b) DDT c) Inorganic Peroxides d) Nitrate e) Magnesium in Plasma f) PyrophosphateSection C: Measurement of Enzyme Activity Preliminary Remarks I. The Importance of the Measurement of Enzyme Activity a) In Medicine b) In Food Chemistry c) In Botanical and Agricultural Chemistry II. Methods 1. Aldolases 2. Dehydrogenases 3. Esterases 4. Proteases 5. Transaminases 6. Others III. Histochemical Detection of Enzymes 1. Introduction (Aims of the Method) 2. General 3. Special MethodsSection D: Biochemical Reagents I. Enzymes II. Coenzymes and Substrates III. Complete Reagent KitsTables Conversion Table: Transmittance ⇌ Extinction Atomic WeightsIndex