Methods in Virology

Contributors

Preface

Contents of Previous Volumes

Chapter 1. Hybridization of Viral Nucleic Acids: Newer Methods on Solid Media and in Solution

I. Introduction

II. Purification of Nucleic Acid

III. Preparation of Labeled Nucleic Acid Probes

IV. Basic Methods of Nucleic Acid Hybridization

V. Southern Blot Hybridization

VI. Northern Blots

References

Chapter 2. Applications of Oligonucleotide Fingerprinting to the Identification of Viruses

I. Introduction

II. Principles and Mechanisms of Fingerprint Analysis

III. Experimental Procedures

IV. Applications

V. Summary and Conclusions

References

Chapter 3. Immunosorbent Electron Microscopy in Plant Virus Studies

I. Introduction

II. Parameters of the Method

III. Varying Success with ISEM

IV. Applications

V. Detection of Double-Stranded RNA

VI. Preparation of Grids for Later Use

VII. Problems and Prospects for the Future

References

Chapter 4. Quantitative Transmission Electron Microscopy for the Determination of Mass-Molecular Weight of Viruses

I. Introduction

II. Theory

III. Procedure and Treatment of Data to Obtain the Average Dry Mass of Virus Particles

IV. Applications in Virology

V. Discussion

VI. Summary

References

Chapter 5. Use of Thin Sectioning for Visualization and Identification of Plant Viruses

I. Introduction

II. Technical Procedures for Processing Plant Tissues

III. Cytological Modifications of Diagnostic Value

References

Chapter 6. Purification and Immunological Analyses of Plant Viral Inclusion Bodies

I. Introduction

II. Inclusion Purification

III. SerologicalAnalyses

IV. Discussion

References

Chapter 7. Use of Mosquitoes to Detect and Propagate Viruses

I. Rationale for Use of the Technique

II. Technical Considerations

III. Possible Limitations and Pitfalls

References

Chapter 8. Prions: Methods for Assay, Purification, and Characterization

I. Introduction

II. Assays for Scrapie Prions

III. Histopathology

IV. Purification

V. Identification and Detection of PrP

VI. Electron Microscopy

VII. Polarization Microscopy

VIII. Biocontainment and Decontamination Procedures

References

Chapter 9. Detection of Genome-Linked Proteins of Plant and Animal Viruses

I. Introduction

II. Detection of a Genome-Linked Protein (VPg)

III. Characterization of Genome-Linked Proteins and Adjacent Nucleotide Sequences

IV. Biosynthesis of Genome-Linked Proteins

V. Possible Functions of Genome-Linked Proteins

References

Index