Methods in Virology
Volume V
- 1st Edition - June 28, 2014
- Editors: Karl Maramorosch, Hilary Koprowski
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 4 3 1 7 - 7
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 6 2 2 3 - 9
Methods in Virology, Volume V focuses on the methods used in virology, including hybridization, gel electrophoresis, freeze-etching technique, and ultracentrifugation. The… Read more

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Request a sales quoteMethods in Virology, Volume V focuses on the methods used in virology, including hybridization, gel electrophoresis, freeze-etching technique, and ultracentrifugation. The selection first offers information on the fusion of cells for virus studies and production of cell hybrids; approaches to ultracentrifugation; and polyacrylamide gel electrophoresis of viral RNA. Discussions focus on applications to virological problems and analysis of results; analysis of the distribution of RNA on polyacrylamide gels after electrophoresis; and biological analysis of DNA components. The book then examines the polyacrylamide gel electrophoresis of viral proteins, DNA-RNA and DNA-DNA hybridization in virus research, and techniques of RNA-DNA hybridization in solution for the study of viral transcription. Topics include preparation of nucleic acids, hybridization and elution procedures that minimize RNA degradation, and procedures for acrylamide gel electrophoresis. The text takes a look at freeze-etching technique for the study of virus ultrastructure; procedures to increase virus yield from infected plants; and the immunoperoxidase technique. Concerns include principles of the immunoperoxidase technique, histochemical detection of peroxidase activity, sequence of events in virus infection, and factors affecting virus yield. The selection is a valuable source of data for researchers interested in the methods employed in virology.
List of Contributors
Preface
Contents of Other Volumes
Chapter 1—Fusion of Cells for Virus Studies and Production of Cell Hybrids
I. Introduction
II. Methods
III. Cell Fusion in Virology
IV. Selection of Hybrid Cell Lines
V. Uses of Heterokaryons and Hybrid Cell Lines
References
Chapter 2—New Approaches to Ultracentrifugation
I. Introduction
II. A Solution: New Approaches
III. Results
IV. Discussion and Summary
Acknowledgments
References
Chapter 3—Small Circular Viral DNA: Preparation and Analysis
I. Introduction
II. Virus Cultivation, Concentration, and Purification
III. DNA Extraction and Purification
IV. Physical Analysis of DNA Components
V. Biological Analysis of DNA Components
Appendix
Acknowledgments
References
Chapter 4—Polyacrylamide Gel Electrophoresis of Viral RNA
I. Introduction
II. Apparatus
III. Procedures for Preparation and Running of Gels
IV. Analysis of the Distribution of RNA on Polyacrylamide Gels after Electrophoresis
V. Elution of RNO from Analytical Gel Fractions
VI. Large-Scale Preparative Techniques
VII. Applications to Virological Problems and the Analysis of Results
VIII. Conclusion
Acknowledgments
References
Chapter 5—Polyacrylamide Gel Electrophoresis of Viral Proteins
I. Introduction
II. Procedures for Acrylamide Gel Electrophoresis
III. Interpretation of Results
IV. Discussion
V. Appendix: Common Complaints, Troubleshooting Suggestions, and Tips
References
Chapter 6—DNA-RNA and DNA-DNA Hybridization in Virus Research
I. Introduction
II. Materials
III. DNA-RNA Hybridization Procedure
IV. Characteristics of the DNA-RNA Hybridization Reaction
V. Thermal Elution of RNA from RNA-DNA Hybrids
VI. Hybridization and Elution Procedure that Minimizes RNA Degradation
VII. DNA-DNO Hybridization
VIII. Alternative Methods of Hybridization
Acknowledgment
References
Chapter 7—Techniques of RNA-DNA Hybridization in Solution for the Study of Viral Transcription
I. Introduction
II. Basic Equipment
III. Preparation of Nucleic Acids
IV. RNA-DNA Hybridization in Solution (Liquid Hybridization)
V. Segmental Transcription Studies by RNA-DNA Hybridization in Solution
References
Chapter 8—Methods for the Study of mRNA Synthesis in Bacteriophage-Infected E. coli
I. General Introduction
II. Bacteriophage-Host System
III. Isolation Methods
IV. Analytical Methods
Acknowledgments
References
Chapter 9—Reactions of Viruses in Agar Gel
I. Introduction
II. Reactions Between Viral Antigens and Antibiotics
III. Reactions Between Viruses and Erythrocyte Stromata
IV. Electron Microscopic Examination of Reaction Lines
Acknowledgments
References
Chapter 10—Methods for the Study of Virus-Antibody Complexes
I. General Introduction
II. Methods of Reactivating Neutralized Virus
III. Characterization of the Virus-Antibody Complex
IV. Methods of Separating the Components of a Virus-Antibody Reaction Mixture
References
Chapter 11—Techniques of Ferritin-Tagged Antibodies
I. Introduction
II. Purification of Ferritin
III. Preparation of Antibody Globulin Fraction from Serum
IV. Conjugation of Ferritin and Antibody
V. Evaluating the Conjugate
VI. Methods of Direct Ferritin-Tagging
VII. Methods of Indirect Ferritin-Tagging
VIII. Controls in Ferritin-Tagging Experiments
IX. Hybrid Antibody Method for Ferritin Localization of Antigen
X. Application of Hybrid Antibody
XI. Other Methods of Visualizing Immune Globulins
XII. Conclusions
References
Chapter 12—The Immunoperoxidase Technique: Localization of Viral Antigens in Cells
I. Introduction
II. The Principles of the Immunoperoxidase Technique
III. Properties of Horseradish Peroxidase
IV. Histochemical Detection of Peroxidase Activity
V. Peroxidase-Antibody Labeling Techniques
VI. Procedure for Detection of Viral Antigens in Cells by the Immunoperoxidase Technique
VII. Interpretation of the Results Obtained for the Localization of Viral Antigens in Cells by the Immunoperoxidase Technique
Acknowledgments
References
Chapter 13—Leaf-Dip Serology
I. Introduction
II. Procedure
III. Results and Discussion
References
Chapter 14—Procedure to Increase Virus Yield from Infected Plants
I. Introduction
II. Sequence of Events in Virus Infection
III. Criteria for Virus Yield
IV. Factors Affecting Virus Yield
V. Summary
References
Chapter 15—Freeze-Etching Technique for the Study of Virus Ultrastructure
I. Introduction
II. The Freeze-Etching Technique
III. Results up to the Present
IV. Conclusion and Prospects
Acknowledgments
References
Author Index
Subject Index
- No. of pages: 548
- Language: English
- Edition: 1
- Published: June 28, 2014
- Imprint: Academic Press
- Paperback ISBN: 9781483243177
- eBook ISBN: 9781483262239
KM
Karl Maramorosch
Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.
Affiliations and expertise
Professor, Rutgers University, New Brunswick, NJ, USARead Methods in Virology on ScienceDirect