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Methods in Stem Cell Biology - Part A
- 1st Edition, Volume 170 - July 7, 2022
- Editors: Ilio Vitale, Gwenola Manic, Lorenzo Galluzzi
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 9 0 0 2 0 - 1
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 0 0 2 1 - 8
Methods in Cancer Stem Cell Biology: Part A, Volume 170 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting c… Read more
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Request a sales quoteMethods in Cancer Stem Cell Biology: Part A, Volume 170 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Cell Biology series
- Includes the latest information on the topic of Methods in Cancer Stem Cell Biology
Academic, resercahers, government and industrial sectors
- Cover image
- Title Page
- Table of Contents
- Copyright
- Contributors
- Chapter 1: Orthotopic brain tumor models derived from glioblastoma stem-like cells
- Abstract
- 1: Introduction
- 2: Preparing a GBM stem cell line from a surgical specimen
- 3: Cell maintenance
- 4: Implantation of GSCs
- 5: Monitoring of tumor growth and treatment response
- Acknowledgments
- References
- Chapter 2: Identification and isolation of slow-cycling glioma stem cells
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- 5: Concluding remarks
- Acknowledgments
- References
- Chapter 3: Immunohistochemical analysis of the developing mouse cortex
- Abstract
- 1: Introduction
- 2: Materials and methods
- 3: Conclusion
- 4: Notes
- Acknowledgments
- References
- Chapter 4: Subcellular fractionation of brain tumor stem cells
- Abstract
- 1: Introduction
- 2: Cell maintenance
- 3: Subcellular fractionation
- 4: Evaluation of subcellular fraction purity by Western blotting
- 5: Conclusion remarks
- 6: Notes
- Acknowledgments
- References
- Chapter 5: microRNAs transcriptional profiling of mammary stem cells isolated by PKH26 staining and FACS sorting
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- References
- Chapter 6: Genome-wide RNA interference screen in cancer stem cells
- Abstract
- 1: Introduction
- 2: Global overview
- 3: Preliminary steps
- 4: Cell maintenance
- 5: Collagen coating of culture plates
- 6: Reverse transfection of siRNAs
- 7: ALDEFLUOR assay
- 8: Image acquisition and analysis
- 9: Data pre-processing and candidate selection
- 10: Notes
- References
- Chapter 7: Clonal analysis of human clonogenic keratinocytes
- Abstract
- 1: Introduction: The meaning and importance of the clonal analysis
- 2: Materials
- 3: Methods
- 4: Concluding remarks
- Acknowledgments
- References
- Chapter 8: Immunofluorescence analysis of myogenic differentiation
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Immunofluorescence staining of the cells
- 5: Notes
- Acknowledgments
- References
- Chapter 9: Generating pancreatic beta-like cells from human pluripotent stem cells
- Abstract
- 1: Introduction
- 2: Culture and differentiation of hPSCs
- 3: Transplantation into kidney capsule of NOD/SCID mice for in vivo maturation
- 4: Glucose-stimulated insulin secretion (GSIS) assay
- 5: Concluding remarks
- 6: Notes
- References
- Chapter 10: In vitro preparation of human Dental Pulp Stem Cell grafts with biodegradable polymer scaffolds for nerve tissue engineering
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- 5: Concluding remarks
- References
- Chapter 11: Identification of progenitor cells and their progenies in adult Drosophila midgut
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Assessment of proliferation and differentiation capability of ISCs
- 5: Concluding remarks
- 6: Notes
- Acknowledgment
- References
- Chapter 12: Generation and characterization of human mesenchymal stem/stromal cells for cell therapy applications
- Abstract
- 1: Introduction
- 2: General considerations
- 3: Isolation of MSCs from bone marrow
- 4: Isolation of MSCs from human umbilical cord
- 5: Cryopreservation and thawing MSCs
- 6: Human MSCs characterization
- 7: Expected results
- References
- Chapter 13: Generation of sex-reversed female clonal mice via CRISPR/Cas9-mediated Y chromosome deletion in male embryonic stem cells
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- 5: Concluding remarks
- References
- No. of pages: 226
- Language: English
- Edition: 1
- Volume: 170
- Published: July 7, 2022
- Imprint: Academic Press
- Hardback ISBN: 9780323900201
- eBook ISBN: 9780323900218
IV
Ilio Vitale
Dr Ilio Vitale is an esteemed oncologist. He is a member of the University of Rome and associated to the Regina Elena National Cancer Institute based in Italy.
Affiliations and expertise
Regina Elena National Cancer Institute, Rome and Department of Biology, University of Rome, ItalyGM
Gwenola Manic
Gwenola Manic received her Ph.D. from the University of Paris Sud/Paris XI (France) in 2012 studying the impact of the design of HIV-1-derived vectors and DNA repair machinery on lentiviral expression. At present she is working as a postdoctoral fellow at the Department of Biology at the Università degli Studi di Roma “Tor Vergata” in Rome (Italy) on the contribution of chromosomal instability in tumorigenesis and the therapeutic targeting of cancer stem cells.
Affiliations and expertise
Candiolo Cancer Institute, FPO -IRCCs, Candiolo, ItalyLG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USARead Methods in Stem Cell Biology - Part A on ScienceDirect