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Methods in Psychobiology
Specialized Laboratory Techniques in Neuropsychology and Neurobiology
1st Edition - January 1, 1972
Editor: R. D. Myers
eBook ISBN:9781483268293
9 7 8 - 1 - 4 8 3 2 - 6 8 2 9 - 3
Methods in Psychobiology, Volume 2, Specialized Laboratory Techniques in Neuropsychology and Neurobiology is intended for the beginning ""student"" in physiological, neuro-,… Read more
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Methods in Psychobiology, Volume 2, Specialized Laboratory Techniques in Neuropsychology and Neurobiology is intended for the beginning ""student"" in physiological, neuro-, bio-psychology, or whatever label one wishes to attach to the exciting interdisciplinary field which weds the brain and behavior. In contrast to Volume 1, somewhat more emphasis is given in the selection of topics to a number of difficult behavioral methods that are used frequently by individuals in the more traditional neurosciences. The book begins with a discussion of the measurement of behavioral activity. This is followed by separate chapters on techniques such as electric shock motivation; aversive learning; methods of assessing the behavioral effects of drugs; long-term intravenous infusions; and perfusion of different parts of the brain. Subsequent chapters deal with the assay of pharmacologically active substances; the split-brain technique; using microknives in brain lesion studies and the production of isolated brain-stem islands; the functional decortication technique; and recording evoked potentials.
List of Contributors
Preface
Chapter 1 Measuring Behavioral Activity
I. Introduction
II. Methods of Measurement
III. The Revolving Wheel
IV. Stabilimeter and Stationary-Cage Recording
V. Conclusions
Acknowledgment
References
Appendix
Chapter 2 Techniques of Electric Shock Motivation
I. Apparatus for Delivering the Shock to the Subject
II. Shock Sources: General Considerations
III. Types of Shock Sources
IV. Shock Scramblers
V. Factors Which Can Attenuate Grid Floor Shock
VI. Basic Techniques for the Measurement and Control of Electric Shock Motivation
References
Chapter 3 Aversive Learning Situations: Apparatus and Procedures
I. Introduction
II. Instrumental Aversive Situations
References
Appendix
Chapter 4 Assessing the Effect of Drugs
I. Introduction
II. Pharmacological Considerations
III. Behavioral Considerations
References
Appendix
Chapter 5 Manipulation of the Oral and Gastric Environments
I. Origin and Application of Techniques
II. Methods
III. Future Applications
Acknowledgments
References
Appendix
Chapter 6 Long-Term Intravenous Infusion
I. Introduction
II. Saddle and Feedthrough Swivel
III. Cannula Construction
IV. Surgical Procedure
V. Trouble Shooting and Special Arts
References
Appendix
Chapter 7 Methods for Perfusing Different Structures of the Brain
I. Introduction
II. Perfusing the Cerebral Ventricles
III. Isolated Tissue Perfusion with Push-Pull Cannulae
IV. Perfusion of the Cortex
V. Theoretical Considerations
References
Appendix
Chapter 8 The Neurobiological Assay
I. Introduction
II. Identification by Pharmacological Analysis
III. Recording the Contractions of Isolated Smooth and Striated Muscles
IV. The Bioassay of Acetylcholine
V. The Bioassay of 5-Hydroxytryptamine (Serotonin)
VI. The Bioassay of Catecholamines
References
Appendix
Chapter 9 Specialized Lesions: the Split-Brain Technique
I. Historical Introduction
II. Surgical Methods
III. Post-Surgical Care
IV. Histological Verification of Surgery
V. Fabrication of Instruments for Split-Brain Surgery
VI. Behavioral Testing Methods
References
Appendix
Chapter 10 Specialized Lesions: "Cerveau Isolé" and "Encephale Isolé"
I. Introduction
II. The "Cerveau Isolé"
III. The "Encephale Isolé"
IV. Maintenance and Nursing Care
V. The Use of the Preparations for Psychobiology
References
Appendix
Chapter 11 The Use of Microknives in Brain Lesion Studies and the Production of Isolated Brain-Stem Islands
I. Brain Lesions: General Considerations
II. The Usefulness of Microknives
III. The Hypothalamic Island
IV. Design and Construction of Knives
V. The Hypothalamic Island: Procedures and Post-Operative Care
References
Appendix
Chapter 12 Inducing Cortical Spreading Depression
I. Introduction
II. Semi-Chronic Technique
III. Chronic Technique
IV. Main Fields of Application
References
Appendix
Chapter 13 Recording Evoked Potentials
I. Introduction
II. The Recording Situation
III. Stimulus Generators
IV. Installation of Electrodes in Humans and Animals