Methods in iPSC Technology
- 1st Edition - June 2, 2021
- Imprint: Academic Press
- Editor: Alexander Birbrair
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 8 5 7 6 6 - 6
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 8 5 6 4 4 - 7
The series Advances in Stem Cell Biology is a timely and expansive collection of comprehensive information and new discoveries in the field of stem cell biology. Method… Read more
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Request a sales quoteThe series Advances in Stem Cell Biology is a timely and expansive collection of comprehensive information and new discoveries in the field of stem cell biology.
Methods in iPSC Technology, Volume 9 addresses the different methods used for induced pluripotent stem cell (iPSC) formation, maintenance, expansion, and differentiation.
The ability to reprogram different cell types to iPSCs offers an opportunity to generate pluripotent patient-specific cell lines that can help in the understanding of multiple human disorders. This volume addresses a variety of current methods used in the generation and manipulation of iPSCs, such as magnetic nanoparticles, piggyBac vectors, lentiviral vectors, bioscaffolds, somatic cell nuclear transfer, CRISPR/Cas9, bacteria, and much more.
This volume is written for researchers and scientists interested in stem cell therapy, cell biology, regenerative medicine, and organ transplantation and is contributed by world-renowned authors in the field.
Methods in iPSC Technology, Volume 9 addresses the different methods used for induced pluripotent stem cell (iPSC) formation, maintenance, expansion, and differentiation.
The ability to reprogram different cell types to iPSCs offers an opportunity to generate pluripotent patient-specific cell lines that can help in the understanding of multiple human disorders. This volume addresses a variety of current methods used in the generation and manipulation of iPSCs, such as magnetic nanoparticles, piggyBac vectors, lentiviral vectors, bioscaffolds, somatic cell nuclear transfer, CRISPR/Cas9, bacteria, and much more.
This volume is written for researchers and scientists interested in stem cell therapy, cell biology, regenerative medicine, and organ transplantation and is contributed by world-renowned authors in the field.
- Provides overview of the fast-moving field of stem cell biology and function, regenerative medicine, and therapeutics
- Covers the different methods used for iPSC formation, maintenance, expansion, and differentiation
- Is contributed by world-renowned experts in the field
Researchers and scientists in stem cell therapy, cell biology, regenerative medicine, and organ transplantation
Graduate and undergraduate students in the above fields
Graduate and undergraduate students in the above fields
- Cover image
- Title page
- Table of Contents
- Advances in Stem Cell Biology
- Copyright
- Dedication
- Contributors
- About the editor
- Preface
- Chapter 1. Current reprogramming methods to generate high-quality iPSCs
- Introduction
- Reprogramming methods (Table 1.1)
- Reprogramming factors
- Commentary
- Chapter 2. Using magnetic nanoparticles in iPSCs
- Introduction
- Nanotechnology and nanoparticles
- Biomedical applications of magnetic nanoparticles
- Transgene delivery to hiPSCs using magnetic nanoparticles
- Transfection of hiPSC-CMs using magnetic nanoparticles
- Conclusions and perspectives
- Chapter 3. PiggyBac vectors in pluripotent stem cell research and applications
- From a moth transposon to the piggyBac vector
- Improved piggyBac systems for transgene expression in PSCs
- Use of piggyBac vectors in PSCs
- Concluding remarks and future perspectives: piggyBac in the genome editing era
- Chapter 4. Lentiviral vectors as the delivery vehicles for transduction into iPSCs: shortcomings and benefits
- Chapter 5. Decellularized liver extracellular matrix for iPSC-based liver engineering
- Introduction
- Development of decellularized liver matrix
- iPSCs as a cell source for liver engineering
- Liver engineering with decellularized matrix and iPSCs
- Future directions
- Chapter 6. Combining bioscaffolds and iPSCs in the treatment of neural trauma and Alzheimer’s disease
- Introduction
- Background of induced pluripotent stem cells
- The investigation of bioscaffolds and iPSC in spinal cord and peripheral nerve injuries
- Induced pluripotent stem cell and bioscaffolds for the therapy for Alzheimer’s disease
- Chapter 7. Emerging strategies for scalable human induced pluripotent stem cell expansion and differentiation
- Introduction
- General considerations for hiPSC bioreactor culture
- Novel bioreactors for hiPSC expansion and differentiation
- Conclusions and future trends
- Chapter 8. One plus one could be greater than two: combining the powers of somatic cell nuclear transfer with Yamanaka’s factors in generating clinical grade human pluripotent stem cells
- A brief history of somatic cell nuclear transfer
- The “Magic” egg
- Epigenetics: memories make a cell
- Transcription factors: you are what you express!
- Embryonic stem cells
- Induced pluripotent stem cells
- Are iPSCs suitable for human applications?
- Regeneration and cancer: both sides of a coin
- One plus one could be greater than two: iPSC nucleus for nuclear transfer
- iPSCNT and future prognostications
- Chapter 9. Bacteria to form induced pluripotent stem cells
- Introduction
- Origin of eukaryotic cells
- Bacterial influence on cellular homeostasis and plasticity
- Cell reprogramming by lactic acid bacteria (LAB) in vitro
- Prospects
- Conclusion
- Chapter 10. CRISPR/Cas9 technologies to manipulate human induced pluripotent stem cells
- Introduction
- Methodologies for CRISPR/Cas9-mediated genome editing
- Applications of the CRISPR/Cas9 system in human pluripotent stem cells
- Conclusion and future directions
- Chapter 11. Scalable expansion of human pluripotent stem cells for biomanufacturing cellular therapeutics
- Introduction
- Cultivation environment and hPSC physiology
- Challenges and conclusions
- Chapter 12. Xeno-free cultivation of human induced pluripotent stem cells for clinical applications
- Introduction
- Historical overview of stem cells
- Human pluripotent stem cells
- Culture of human pluripotent stem cells
- Clinical manufacturing
- Clinical trials
- Cell banks
- Future prospects
- Index
- Edition: 1
- Published: June 2, 2021
- No. of pages (Paperback): 370
- No. of pages (eBook): 370
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9780323857666
- eBook ISBN: 9780323856447
AB
Alexander Birbrair
Dr. Alexander Birbrair received his bachelor’s biomedical degree from Santa Cruz State University in Brazil. He completed his PhD in Neuroscience, in the field of stem cell biology, at the Wake Forest School of Medicine under the mentorship of Osvaldo Delbono. Then, he joined as a postdoc in stem cell biology at Paul Frenette’s laboratory at Albert Einstein School of Medicine in New York. In 2016, he was appointed faculty at Federal University of Minas Gerais in Brazil, where he started his own lab. His laboratory is interested in understanding how the cellular components of different tissues function and control disease progression. His group explores the roles of specific cell populations in the tissue microenvironment by using state-of-the-art techniques. His research is funded by the Serrapilheira Institute, CNPq, CAPES, and FAPEMIG. In 2018, Alexander was elected affiliate member of the Brazilian Academy of Sciences (ABC), and, in 2019, he was elected member of the Global Young Academy (GYA), and in 2021, he was elected affiliate member of The World Academy of Sciences (TWAS). He is the Founding Editor and Editor-in-Chief of Current Tissue Microenvironment Reports, and Associate Editor of Molecular Biotechnology. Alexander also serves in the editorial board of several other international journals: Stem Cell Reviews and Reports, Stem Cell Research, Stem Cells and Development, and Histology and Histopathology.
Affiliations and expertise
Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
Department of Radiology, Columbia University Medical Center, Medical Center, USARead Methods in iPSC Technology on ScienceDirect