Methodology

List of Contributors

General Preface

Preface to Volume 13


1 Immunochemistry for Enzymology

I. Introduction

II. Production of Antibodies

III. Immunochemical Studies of Proteins

IV. Application of Immunochemistry to Studies of Enzyme Induction and Degradation

V. Immunochemical Studies of Low-Molecular-Weight Metabolites

VI. Conclusions

References


2 The Use of Mutants for the Study of Plant Metabolism

I. Introduction

II. Analysis of Metabolic Pathways

III. Analysis of Regulatory Mechanisms

IV. New Techniques

V. Concluding Remarks

References


3 The Use of Plant Cell Cultures in Studies of Metabolism

I. Introduction

II. Isolation and Characterization of Variant Cell Lines in Culture

III. Metabolism of Amino Acids

IV. Metabolism of Amino Acid-Derived Secondary Products

V. Conclusions

References


4 The Application of Mass Spectrometry to Biochemical and Physiological Studies

I. Introduction

II. Isotopic Discrimination

III. Studies with 18O (with Asides on Other Isotopes of O)

IV. Studies with 2H (D) with a Brief Comparison with 3H (T)

V. Studies with, 15N (with Comments on 13N, C2H2, 36CIO3-, and 14CH3NH3+)

VI. Studies with 13C (with Comments on 11C and 14C)

VII. Conclusions

References


5 NMR in Plant Biochemistry

I. Introduction

II. Theory of Fourier-Transform NMR

III. Measurements Possible with NMR Spectroscopy

IV. Practical Aspects of In Vivo NMR

V. In Vivo 31 P-NMR Spectroscopy of Plants

VI. Studies of Ion Transport and Metabolism

VII. Studies of Metabolic Pathways

References


6 Electron Spin Resonance

I. Introduction to the ESR Method

II. Naturally Occurring Paramagnetic Centers

III. Applications to Plant Metabolism

IV. Concluding Remarks

V. Glossary

References

Index

Contents of Other Volumes