Mechanotransduction

1st Edition, Volume 126 - July 23, 2014
Imprint: Academic Press
Editors: Adam J. Engler, Sanjay Kumar
Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 3 9 4 6 2 4 - 9
eBook ISBN:
9 7 8 - 0 - 1 2 - 3 9 8 3 2 7 - 5

Progress in Molecular Biology and Translational Science provides a forum for discussion of new discoveries, approaches, and ideas in molecular biology. It contains contribut… Read more

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Progress in Molecular Biology and Translational Science provides a forum for discussion of new discoveries, approaches, and ideas in molecular biology. It contains contributions from leaders in their fields and abundant references.

Volume 126 features in-depth reviews that focus on the tools required to investigate mechanotransduction. Additional chapters focus on how we can use these tools to answer fundamental questions about the interaction of physical forces with cell biology, morphogenesis, and function of mature structures. Chapters in the volume are authored by a unique combination of cell biologists and engineers, providing a range of perspectives on mechanotransduction.

Preface
Part One: Subcellular Tools for Activating and Measuring Mechanotransductive Signaling
- Chapter One: The Detection and Role of Molecular Tension in Focal Adhesion Dynamics
  - Abstract
  - 1 Brief Introduction to Mechanobiology
  - 2 Focal Adhesions in Mechanosensing
  - 3 Design and Use of Optically Based Molecular Tension Sensors
  - 4 The Role of Molecular Tension in Focal Adhesion Dynamics
  - 5 Future Outlook
  - Acknowledgments
- Chapter Two: Single-Cell Imaging of Mechanotransduction in Endothelial Cells
  - Abstract
  - 1 Introduction
  - 2 Atherosclerosis, EC Wound Healing, and Mechanotransduction
  - 3 Signaling Molecules Involved in Mechanosensing and Mechanotransduction
  - 4 The Effect of Subcellular Structure on Mechanotransduction
  - 5 Focal Adhesion and FAK
  - 6 Tools to Monitor Signal Transduction in Live Cells
  - 7 Conclusion
Part Two: Focal Adhesions as Sensors
- Chapter Three: Focal Adhesions Function as a Mechanosensor
  - Abstract
  - 1 Introduction: The Basic Organization of Focal Adhesions
  - 2 Mechanosensitivity of Focal Adhesions
  - 3 Focal Adhesions and the Effects of Environmental Parameters
  - 4 Focal Adhesion Signals and Cell Migration
  - Acknowledgments
- Chapter Four: Mechanosensation: A Basic Cellular Process
  - Abstract
  - 1 Introduction
  - 2 Focal Adhesions
  - 3 Conclusions
  - Acknowledgments
- Chapter Five: Mechanical Cues Direct Focal Adhesion Dynamics
  - Abstract
  - 1 Introduction
  - 2 Form and Function of Focal Adhesions
  - 3 AFM as a Tool to Stimulate a Cellular Response
  - 4 Future Directions
  - Acknowledgments
- Chapter Six: Molecular Mechanisms Underlying the Force-Dependent Regulation of Actin-to-ECM Linkage at the Focal Adhesions
  - Abstract
  - 1 Introduction
  - 2 Molecular Assembly in the Actin–Integrin–ECM Linkage
  - 3 Force-Sensing/Transducing Molecules in the Regulation of the Actin–Integrin–ECM Linkage
  - 4 Dynamic Aspect of the Actin–Integrin–ECM Linkage: Molecular Clutch
  - 5 Concluding Remarks
  - Acknowledgments
Part Three: Nuclear Mechanisms of Sensing
- Chapter Seven: The Cellular Mastermind(?)—Mechanotransduction and the Nucleus
  - Abstract
  - 1 Introduction
  - 2 Overview of Nuclear Structure and Organization
  - 3 Mechanically Induced Changes in Nuclear Structure
  - 4 Potential Mechanisms for Direct Nuclear Mechanosensing
  - 5 Mechanotransduction Signaling in the Nucleus
  - 6 Functional Consequences of Impaired Mechanotransduction and Disease
  - 7 Open Questions and Future Research Directions
  - 8 Conclusions
  - Acknowledgments
- Chapter Eight: Nuclear Forces and Cell Mechanosensing
  - Abstract
  - 1 Introduction
  - 2 Cytoskeletal Forces are Exerted on the Nucleus
  - 3 The LINC Complex Transmits Cytoskeletal Forces to the Nuclear Surface
  - 4 The Role of the Nucleus in Cell Mechanosensing
  - 5 Conclusions
  - Acknowledgments
Part Four: Mechano-Sensing in Stem Cells
- Chapter Nine: From Stem Cells to Cardiomyocytes: The Role of Forces in Cardiac Maturation, Aging, and Disease
  - Abstract
  - 1 Introduction
  - 2 Cardiac Morphogenesis During the Lifespan of the Heart
  - 3 Mechanosensitive Compartments in Cardiomyocytes
  - 4 The Sarcomere
  - 5 Other Intracellular Mechanosensitive Structures
  - 6 ECM and Mechanosensing
  - 7 The Influence of Mechanotransduction on Applications of Cardiac Regeneration
  - 8 Conclusion
- Chapter Ten: Matrix Regulation of Tumor-Initiating Cells
  - Abstract
  - 1 Introduction
  - 2 Identification and Isolation of TICs
  - 3 Role of Extracellular Matrix and Mechanical Signals in Regulating TIC Function
  - 4 Conclusion
- Chapter Eleven: Biomaterials Approaches in Stem Cell Mechanobiology
  - Abstract
  - 1 Introduction
  - 2 Mechanical Regulation of Stem Cell Fate
  - 3 Mechanosensing and Mechanotransduction
  - 4 Pushing Ahead: Biomaterials Approaches to Probe Stem Cell Mechanobiology
  - 5 Summary and Outlook
Part Five: Multi-Cellular Sensing
- Chapter Twelve: Mechanotransduction in C. elegans Morphogenesis and Tissue Function
  - Abstract
  - 1 Intracellular Sensation and Response to Mechanical Input
  - 2 Mechanical Influences in Embryonic Development
  - 3 Mechanical Influences in Larval Development and Tissue Function
  - 4 Tools for Manipulation and Imaging
  - 5 Future Prospects
  - Acknowledgments
- Chapter Thirteen: Mechanical Force Sensing in Tissues
  - Abstract
  - 1 Introduction: Molecular Mechanisms of Multicellular Force Sensing
  - 2 Multicellular Sensing During Tissue Growth
  - 3 Multicellular Sensing During Tissue Morphogenesis
  - Acknowledgments
Index

Adam J. Engler

Adam J. Engler is a professor of Bioengineering at UC San Diego and is affiliated with the Material Science and Biomedical Sciences Programs. He also is a resident scientist at the Sanford Consortium for Regenerative Medicine. His research focuses on how physical properties of the niche influence stem cell function and misregulate muscle function and heart performance during disease and aging. Dr. Engler earned his B.S.E. degree in bioengineering and a Ph.D. in mechanical engineering and applied mechanics at the University of Pennsylvania in the lab of Dr. Dennis Discher. Dr. Engler then moved to Princeton University's Department of Molecular Biology as a Postdoctoral Research Fellow in the lab of Dr. Jean Schwarzbauer where his work was funded by the National Cancer Institute. Dr. Engler is the 2008 recipient of the Rupert Timpl and Rita Schaffer Young Investigator Awards from the International Society for Matrix Biology and the Biomedical Engineering Society, respectively. He is also a 2009 NIH Innovator Award recipient, a 2010 Young Investigator Awardee from the Human Frontier Science Program, and a 2013 IDEA Awardee from the Dept. of Defense.

Affiliations and expertise

University of California, San Diego, USA

Sanjay Kumar

Sanjay Kumar is Associate Professor of Bioengineering, Chair of the UC Berkeley & UCSF Graduate Program at UC Berkeley, Faculty Scientist at Lawrence Berkeley National Laboratory and Faculty Director of the UC Berkeley & UCSF Master of Translational Medicine Program. He earned a B.S. in chemical engineering (1996) from the University of Minnesota and then moved on to Johns Hopkins University, where he earned an M.D. (2003) and a Ph.D. in molecular biophysics (2003). Since joining the UC Berkeley faculty in 2005, Dr. Kumar has been fortunate to receive a number of honors, including the Presidential Early Career Award for Scientists and Engineers (PECASE), The NIH Director's New Innovator Award, The Arnold and Mabel Beckman Young Investigator Award, the NSF CAREER Award, the Hellman Family Faculty Fund Award, and the Stem Cells Young Investigator Award.

Affiliations and expertise

University of California, Berkeley, USA

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