MCB: CAR T Cells: Development, Characterization and Applications
- 1st Edition, Volume 167 - February 11, 2022
- Imprint: Academic Press
- Editors: Lorenzo Galluzzi, Sheila Spada
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 8 5 5 0 1 - 3
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 8 5 5 0 2 - 0
MCB: CAR T Cells: Development, Characterization and Applications, Volume 167 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume pr… Read more
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Request a sales quoteMCB: CAR T Cells: Development, Characterization and Applications, Volume 167 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy, Determination of the Biodistribution of Chimeric Antigen Receptor-Modified T Cells against CD19 in NSG Mice, Generation of CAR-T cells using lentiviral vectors, Generation of CAR T-cells using γ-retroviral vector, Flow cytometry detection and quantification of CAR T cells into solid tumors, Evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Cell Biology series
- Includes the latest information on the topic of development, characterization and applications in CAR T Cells
Academic, researchers, government and industrial sectors
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Chapter 1: High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR-T cell therapy
- Abstract
- 1: Introduction
- 2: Construction of AAV transgene vector
- 3: Production of AAV with high titer
- 4: In vitro transcription of Cpf1 mRNA
- 5: Generation of CAR-T cells
- 6: Functional assays
- 7: Concluding remarks
- 8: Notes
- Acknowledgments
- References
- Chapter 2: Determination of the biodistribution of chimeric antigen receptor-modified T cells against CD19 in NSG mice
- Abstract
- 1: Introduction
- 2: Study design
- 3: In vivo optical imaging
- 4: FCM for detecting CD3+/CAR+ cells
- 5: Pathological examination of the tissue distribution of CAR-T cells
- 6: Quantitative PCR for detecting the sequence of CAR-T DNA in tissues
- 7: Statistical analysis
- 8: Conclusion remarks
- Acknowledgments
- References
- Chapter 3: Generation of CAR-T cells using lentiviral vectors
- Abstract
- 1: Introduction
- 2: Selection of targets for CARs
- 3: Design of CARs
- 4: Gene delivery systems for the generation of CAR-T cells
- 5: Generation of CAR-T cells
- 6: Detection of activation/cytotoxicity of CAR-T cells
- 7: Protocols
- Acknowledgments
- References
- Chapter 4: Phenotyping of CAR T cells
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- References
- Chapter 5: Evaluation of CAR-T cell cytotoxicity: Real-time impedance-based analysis
- Abstract
- 1: Introduction
- 2: CAR-T cell cytotoxicity assays
- 3: xCELLigence assay
- 4: Data analysis
- 5: Trouble shooting
- Acknowledgments
- References
- Chapter 6: Flow cytometry detection and quantification of CAR T cells into solid tumors
- Abstract
- 1: Background
- 2: Results
- 3: Discussion
- 4: Conclusion
- 5: Methods
- 6: Preparation of required solutions
- 7: Experimental procedure
- References
- Chapter 7: Generation and functional characterization of CAR exosomes
- Abstract
- 1: Introduction
- 2: Activation, expansion, and transduction of T cells
- 3: Generation of CAR-exosomes
- 4: Characterization of CAR-exosomes
- 5: Cytolytic activity of CAR exosomes
- 6: In vivo study
- References
- Chapter 8: Preclinical testing of CAR T cells in zebrafish xenografts
- Abstract
- 1: Introduction
- 2: Cell culture
- 3: Preparation of lentiviral particles and transduction of cell lines
- 4: Preparation of cell solutions
- 5: Analysis of CAR T cell-mediated killing of cancer cells in vitro
- 6: Preparing zebrafish embryos for xenotransplantation
- 7: Xenotransplantation of Nalm-6 and CAR T cells into circulation of zebrafish embryos
- 8: Imaging of xenotransplanted zebrafish embryos
- 9: Analysis of CAR T cell-mediated killing of cancer cells
- 10: Whole-mount immunostaining of xenotransplanted zebrafish larvae
- 11: Concluding remarks
- 12: Notes
- Acknowledgments
- References
- Chapter 9: In vivo experimental mouse model to test CD19CAR T cells generated with different methods
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- Acknowledgments
- References
- Chapter 10: Non-invasive fluorescence imaging for tracking immune cells in preclinical models of immunotherapy
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- Acknowledgments
- References
- Chapter 11: Generation of CAR T-cells using γ-retroviral vector
- Abstract
- 1: Introduction
- 2: Cell culture and maintenance of 293T cells
- 3: Production of retroviral supernatant (transfection of retroviral construct into 293T cells)
- 4: Retrovirus transduction of T cells
- 5: Concluding remarks
- 6: Notes
- Acknowledgments
- References
- Chapter 12: Methods to monitor in vivo expansion and efficacy of CAR-T cells in preclinical models
- Abstract
- 1: Introduction
- 2: Why monitoring CAR-T expansion and efficacy is significant?
- 3: Monitoring CAR-T persistence and efficacy
- 4: Conclusion
- References
- Chapter 13: Strategies for manufacturing cell therapy products aligned with patient needs
- Abstract
- 1: Introduction
- 2: CAR-T cell manufacturing processes
- 3: Leukapheresis and cell washing
- 4: Enrichment and depletion
- 5: Activation
- 6: Genetic modification of T cells
- 7: Expansion of CAR-T cells
- 8: Harvesting and cryopreservation
- 9: Assessment of CAR-T production methods used in clinical trials
- 10: Challenges and potential solutions in CAR-T design and manufacturing
- 11: Conclusion
- References
- Edition: 1
- Volume: 167
- Published: February 11, 2022
- No. of pages (Hardback): 242
- No. of pages (eBook): 242
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780323855013
- eBook ISBN: 9780323855020
LG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USASS
Sheila Spada
Sheila Spada is at Weill Cornell Medical College, NY, USA
Affiliations and expertise
Department of Radiation Oncology, Weill Cornell Medicine, New York, NY, USARead MCB: CAR T Cells: Development, Characterization and Applications on ScienceDirect