Magnetic Tweezers for the Study of Protein Structure and Function
- 1st Edition, Volume 694 - March 14, 2024
- Imprint: Academic Press
- Editors: Min Ju Shon, Tae-Young Yoon
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 2 2 2 2 9 - 0
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 2 2 2 3 0 - 6
Magnetic Tweezers for the Study of Protein Structure and Function, Volume 694 in the Methods in Enzymology serial highlights new advances in the field, with this new volume presen… Read more
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Request a sales quoteMagnetic Tweezers for the Study of Protein Structure and Function, Volume 694 in the Methods in Enzymology serial highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Single membrane protein tethering for magnetic tweezer experiments, Membrane protein folding studies using a robust magnetic tweezer method, Magnetic tweezers in cell mechanics, and more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology serials
- Updated release includes the latest information on Magnetic Tweezers for the Study of Protein Structure and Function
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- May the force be with proteins
- Chapter One: Magnetic tweezers principles and promises
- Abstract
- 1 Introduction
- 2 Conclusion
- References
- Chapter Two: Use of DNA forceps to measure receptor-ligand dissociation equilibrium constants in a single-molecule competition assay
- Abstract
- 1 Introduction
- 2 Theory
- 3 Numerical simulations
- 4 Single-molecule measurements
- 5 Dissociation equilibrium constant determination
- 6 Discussion
- Acknowledgements
- References
- Chapter Three: Construction and operation of high-resolution magnetic tape head tweezers for measuring single-protein dynamics under force
- Abstract
- 1 Introduction
- 2 Overview of single-molecule magnetic tweezers force spectroscopy
- 3 Construction of the magnetic tape-head tweezers setup
- 4 Operation of the magnetic tape head tweezers software
- 5 Calibration of the setup
- 6 Summary and conclusions
- Acknowledgements
- References
- Chapter Four: High-speed measurements of SNARE–complexin interactions using magnetic tweezers
- Abstract
- 1 Introduction
- 2 Preparation of SNARE–DNA construct
- 3 Magnetic tweezers assay
- 4 Representative results
- 5 Discussion
- Acknowledgment
- References
- Chapter Five: Force-fluorescence setup for observing protein–DNA interactions under load
- Abstract
- 1 Introduction
- 2 Force-fluorescence setup
- 3 Sample preparation
- 4 Force-fluorescence measurements
- 5 Discussion
- Acknowledgments
- References
- Chapter Six: Integrating magnetic tweezers and single-molecule FRET: A comprehensive approach to studying local and global conformational changes simultaneously
- Abstract
- 1 Introduction
- 2 Materials and methods
- 3 Protocols and procedures
- 4 Analysis
- 5 Example
- 6 Note
- Acknowledgments
- References
- Chapter Seven: Horizontal magnetic tweezers and its applications in single molecule micromanipulation experiments
- Abstract
- 1 Introduction
- 2 Instrument configuration
- 3 Method and materials
- Acknowledgments
- References
- Chapter Eight: Magnetic tweezers characterization of the entropic elasticity of intrinsically disordered proteins and peptoids
- Abstract
- 1 Introduction
- 2 Design, synthesis, and attachment of biopolymers for magnetic tweezers experiments
- 3 Force-extension measurements using magnetic tweezers
- 4 Application to the study of a disordered neurofilament subunit protein tail region
- 5 Conclusions
- References
- Chapter Nine: Exploring the free energy landscape of proteins using magnetic tweezers
- Abstract
- 1 Introduction
- 2 Instrumentation
- 3 Design and preparation of protein constructs
- 4 Magnetic tweezers measurement
- 5 Data analysis
- 6 Conclusions and perspectives
- Acknowledgments
- References
- Chapter Ten: Single-molecule tethering methods for membrane proteins
- Abstract
- 1 Introduction
- 2 Overall scheme
- 3 Protein expression and purification
- 4 DNA handle attachment to membrane proteins
- 5 Passivation of coverslip surfaces
- 6 Making single-molecule sample chambers
- 7 Conclusion
- Acknowledgments
- References
- Chapter Eleven: Robust magnetic tweezers for membrane protein folding studies
- Abstract
- 1 Introduction
- 2 Robust membrane protein tweezers
- 3 Two modes of (un)folding experiments
- 4 (Un)folding studies using constant-speed mode
- 5 (Un)folding studies using constant-force mode
- 6 Conclusion
- Acknowledgments
- References
- Chapter Twelve: Magnetic nano-tweezer for interrogating mechanosensitive signaling proteins in space and time
- Abstract
- 1 Introduction
- 2 Chemical synthesis and functionalization of magneto-fluorescent nanoparticles
- 3 Efficient and specific labeling of target cell-surface receptors with magneto-fluorescent nanoparticles
- 4 Spatial and mechanical control of targeted receptors and cell signaling using a micro-magnetic tweezer
- 5 Summary
- Funding source
- References
- Chapter Thirteen: Magnetic tweezers in cell mechanics
- Abstract
- 1 Introduction
- 2 History and principles of the magnetic tweezer
- 3 Mechanosensitive proteins
- 4 3D applications of magnetic tweezers in cells
- 5 Conclusions and future directions
- References
- Edition: 1
- Volume: 694
- Published: March 14, 2024
- No. of pages (Hardback): 372
- No. of pages (eBook): 412
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780443222290
- eBook ISBN: 9780443222306
MS
Min Ju Shon
Min Ju Shon works in the Department of Physics, School of Interdisciplinary Bioscience and Bioengineering at Pohang University of Science and Technology, South Korea.
Affiliations and expertise
Department of Physics, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, South KoreaTY
Tae-Young Yoon
Tae-Young Yoon works in the School of Biological Sciences, Institute for Molecular Biology and Genetics at Seoul National University, South Korea.
Affiliations and expertise
School of Biological Sciences, Institute for Molecular Biology and Genetics, Seoul National University, South KoreaRead Magnetic Tweezers for the Study of Protein Structure and Function on ScienceDirect