Liquid Chromatography

Applications

3rd Edition - April 19, 2023
Editors: Salvatore Fanali, Bezhan Chankvetadze, Paul R. Haddad, Colin Poole, Marja-Liisa Riekkola
Language: English
Paperback ISBN:
9 7 8 - 0 - 3 2 3 - 9 9 9 6 9 - 4
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 9 8 3 0 0 - 6

Liquid Chromatography: Applications, Third Edition delivers a single source of authoritative information on all aspects of the practice of modern liquid chromatography. The text g… Read more

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Liquid Chromatography: Applications, Third Edition delivers a single source of authoritative information on all aspects of the practice of modern liquid chromatography. The text gives those working in academia and industry the opportunity to learn, refresh, and deepen their understanding of the field by covering basic and advanced theoretical concepts, recognition mechanisms, conventional and advanced instrumentation, method development, data analysis, and more. This third edition addresses new developments in the field with updated chapters from expert researchers. The book is a valuable reference for research scientists, teachers, university students, industry professionals in research and development, and quality control managers.

Cover image
Title page
Table of Contents
Copyright
Contributors
Chapter 1: Sample preparation for liquid chromatography
Abstract
1.1: Introduction
1.2: Overview
1.3: Efficient sample extraction techniques for HPLC
1.4: Conclusions
References
Chapter 2: Derivatization in liquid chromatography
Abstract
2.1: Introduction
2.2: Reagent selection
2.3: Post-column reaction detectors
References
Chapter 3: Basic principles for the selection of liquid chromatographic modes for specific applications
Abstract
3.1: Introduction
3.2: Solute properties
3.3: Solvation of stationary phases
3.4: Thermodynamics of solute-sorbent interaction
3.5: LC modes for low-molecular substances
3.6: LC modes for intermediate-sized (oligomeric) molecules
3.7: LC modes for macromolecules
3.8: Outlook and concluding remarks
References
Chapter 4: Amino acid and bioamine separations
Abstract
4.1: Introduction
4.2: Direct separation of amino acids
4.3: Indirect separation of amino acids
4.4: Enantioselective liquid chromatographic analysis of amino acids
4.5: Direct separation of biogenic amines
4.6: Indirect separation of biogenic amines
4.7: Conclusions
References
Chapter 5: Separation of lipids
Abstract
5.1: Introduction
5.2: Lipid classification and properties
5.3: Lipid structures
5.4: Sample preparation for lipid analysis
5.5: Lipid analysis by liquid chromatography
5.6: Concluding remarks
References
Chapter 6: Hyphenated liquid chromatography techniques in analysis of neurotransmitters and their metabolites
Abstract
6.1: Introduction
6.2: Conclusions
References
Chapter 7: Analysis of natural toxins by liquid chromatography
Abstract
Acknowledgments
7.1: Introduction
7.2: Mycotoxins
7.3: Lipophilic marine toxins
7.4: Cyanotoxins
7.5: Tetrodotoxin
7.6: Saxitoxin and analogs
References
Chapter 8: Current LC methods for the separation and analysis of peptides and proteins
Abstract
8.1: Introduction
8.2: Key modes of separation
8.3: Size-exclusion chromatography
8.4: Ion exchange chromatography
8.5: Reversed phase chromatography and its application to proteomics
8.6: Hydrophobic interaction chromatography
8.7: Hydrophilic interaction liquid chromatography
8.8: Multimodal chromatography
8.9: Affinity chromatography
8.10: Ultra-high performance liquid chromatography (UHPLC)
8.11: Capillary liquid chromatography and nano-liquid chromatography
8.12: Chip-liquid chromatography
8.13: Monolithic columns
8.14: Conclusions
References
Chapter 9: Liquid chromatography in proteomics research
Abstract
9.1: Introduction
9.2: Liquid chromatographic techniques in proteomics analysis
9.3: Conclusions
References
Chapter 10: Analysis of oligonucleotides by liquid chromatography
Abstract
10.1: Introduction
10.2: Liquid chromatography
10.3: Conclusion and perspectives
References
Chapter 11: Separation of glycans and monosaccharides
Abstract
11.1: Introduction
11.2: Types of glycans
11.3: Analysis and characterization of glycans
11.4: Quantitative monosaccharide composition analysis
11.5: Conclusion
References
Chapter 12: Metabolic phenotyping (metabonomics/metabolomics) by liquid chromatography-mass spectrometry
Abstract
12.1: Introduction
12.2: LC/MS-based approaches to metabolic phenotyping
12.3: LC/ion mobility and ion mobility spectrometry
12.4: Applying LC/MS to metabolic phenotyping
12.5: Conclusions
References
Chapter 13: Principles, current applications, and future perspectives of liquid chromatography–mass spectrometry in clinical chemistry
Abstract
Dedication
13.1: Introduction
13.2: LC-MS in the routine clinical laboratory
13.3: Sample preparation in clinical LC-MS
13.4: Applications
13.5: Perspectives: Clinical lipidomics
13.6: Final remarks
References
Chapter 14: Application of liquid chromatography for anti-doping testing. Part 1: Anabolic agents and glucocorticoids
Abstract
14.1: Introduction
14.2: Recent trends in HPLC testing for androgenic anabolic steroids
14.3: Recent trends in the HPLC testing for glucocorticoids
14.4: Potential of microsampling and miniaturization in the anti-doping testing of anabolic androgenic steroids and glucocorticoids
References
Chapter 15: Environmental analysis: Atmospheric samples
Abstract
Acknowledgments
15.1: Introduction
15.2: Sampling and sample preparation
15.3: Off-line techniques
15.4: On-line and on-site techniques
15.5: Conclusions
References
Chapter 16: Environmental analysis: Emerging pollutants
Abstract
16.1: Introduction
16.2: General trends
16.3: Target analysis of specific contaminant groups using LC–MS
16.4: Conclusions
References
Chapter 17: Application of liquid chromatography in studies of microplastics
Abstract
Acknowledgments
17.1: Introduction
17.2: Liquid chromatography for microplastic-related contaminant determination
17.3: Analysis of contaminants in microplastics
17.4: Conclusions
References
Chapter 18: Environmental analysis: Persistent organic pollutants
Abstract
Acknowledgments
18.1: Introduction
18.2: Analysis of POPs via liquid chromatography
18.3: Conclusions
References
Chapter 19: Foodomics: LC and LC–MS-based omics strategies in food science and nutrition
Abstract
Acknowledgments
19.1: Introduction
19.2: Fundamentals of omics approaches based on LC
19.3: LC-based Foodomics applications
References
Chapter 20: Compositional analysis of foods
Abstract
Acknowledgments
20.1: Introduction
20.2: Carbohydrates
20.3: Vitamins
20.4: Amino acids, peptides, and proteins
20.5: Lipids
20.6: Minor components of food
20.7: Food additives
20.8: Conclusions and future trends
References
Chapter 21: Multiresidue methods for determination of pesticides and related contaminants in food by liquid chromatography
Abstract
21.1: Introduction
21.2: Regulation and monitoring programs
21.3: Extraction methods
21.4: Method validation
21.5: LC methods
21.6: Analysis of specific compounds
21.7: New advances and future perspectives in multiresidue analysis
References
Chapter 22: Analysis of vitamins by liquid chromatography
Abstract
22.1: Introduction
22.2: Liquid chromatographic determination of water-soluble vitamins
22.3: Liquid chromatographic determination of fat-soluble vitamins
22.4: Multivitamin methods
References
Chapter 23: Determination of veterinary drug residues in foods by liquid chromatography–mass spectrometry: An updated overview of the most recent applications
Abstract
23.1: Introduction
23.2: Options in veterinary residue analysis using LC–MS
23.3: Conclusions
References
Chapter 24: Application of enantioselective liquid chromatography
Abstract
24.1: Introduction
24.2: Analytical applications
24.3: Preparative and product scale separation of enantiomers
24.4: Conclusions
References
Chapter 25: Liquid chromatography in the pharmaceutical industry
Abstract
Acknowledgments
25.1: The role of separation science in pharmaceutical drug development
25.2: Increasing chromatographic resolution
25.3: Chromatographic method development: RPLC
References
Chapter 26: Analysis of pharmaceutically and biologically relevant protein variants
Abstract
Acknowledgments
26.1: Introduction
26.2: Size variants
26.3: Charge variants
26.4: Hydrophobicity variants and amino acid modifications
26.5: Higher order structures
26.6: Analysis of construct specific isoforms
26.7: Advances in the analysis of protein isoforms: Multidimensional/attribute/level methods
26.8: Summary and outlook
References
Chapter 27: Liquid chromatography in forensic toxicology
Abstract
27.1: Introduction
27.2: Compounds of interest in forensic toxicology
27.3: Latest generation techniques
References
Chapter 28: Analysis of natural products by liquid chromatography
Abstract
Acknowledgments
28.1: Introduction
28.2: Discovery of quality markers for TCMs
28.3: Qualitative analysis for TCMs
28.4: Quantitative determination of phytochemicals in TCMs
28.5: Conclusion and perspectives
References
Chapter 29: Speciation and element-specific detection
Abstract
29.1: Introduction
29.2: Sampling
29.3: Sample storage and processing
29.4: Speciation approaches: Direct methods or hyphenated techniques
29.5: Interfacing: nebulizers, use of internal standard, and post-column dilution
29.6: Element-selective detection
29.7: Quantification and quality control
References
Index

Salvatore Fanali

Salvatore Fanali is Director of Research at the Institute of Chemical Methodologies, Italian National Research Council (C.N.R.) in Monterotondo (Rome), Italy, and head of the Capillary Electromigration and Chromatographic Methods Unit at the same Institute. His research activity is mainly focused on separation science including the development of modern miniaturized techniques (electrodriven and liquid chromatography). He also studies hyphenation with mass spectrometry and development of new stationary phases. Separation methods developed are currently applied to food, pharmaceuticals, chiral environment, and biomedical analysis. He is Editor of the Journal of Chromatography A and a member of the advisory editorial board of seven international scientific journals. Fanali is the author of about 300 publications including some book chapters. He received several awards including the “Liberti Medal” in Separation Science from the Italian Chemical Society.

Affiliations and expertise

Committee of the Ph.D. School in Nanoscience and Advanced Technologies, University of Verona, Verona, Italy

Bezhan Chankvetadze

Bezhan Chankvetadze is professor of Analytical Chemistry at Tbilisi State University, Editor of Journal of Pharmaceutical and Biomedical Analysis, and is well recognized in the field of separation science. He is one of the leaders in chromatography and chiral separations with a large number of publications.

Affiliations and expertise

Professor, Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia

Paul R. Haddad

Paul Haddad is currently a Distinguished Professor of Chemistry and Australian Research Council Federation Fellow at the University of Tasmania, as well as Director of the Pfizer Analytical Research Centre. He has more than 500 publications in this field and has presented in excess of 450 papers at local and international scientific meetings. He is an editor of Journal of Chromatography A, a contributing editor for Trends in Analytical Chemistry, and was an editor of Analytica Chimica Acta for 6 years. He is currently a member of the editorial boards of 10 other journals of analytical chemistry or separation science. He is the recipient of several national and international awards, including the ACS Award in Chromatography, the Marcel Golay Award, the AJP Martin Gold Medal awarded by the Chromatographic Society, the Royal Society of Chemistry Analytical Separation Methods Award, the RACI HG Smith and Analytical Division medals, and more.

Affiliations and expertise

Distinguished Professor, School of Natural Sciences, University of Tasmania, Hobart, Australia

Colin Poole

Professor Colin Poole is internationally known in the field of thin-layer chromatography and is an editor of the Journal of Chromatography and former editor of the Journal of Planar Chromatography – Modern TLC. He has authored several books on chromatography, recent examples being The Essence of Chromatography published by Elsevier (2003), and Gas Chromatography published by Elsevier (2012). He is the author of approximately 400 research articles, many of which deal with thin-layer chromatography, and is co-chair of the biennial “International Symposium on High-Performance Thin-Layer Chromatography”.

Affiliations and expertise

Department of Chemistry, Wayne State University, Detroit, MI, USA

Marja-Liisa Riekkola

Marja-Liisa Riekkola is a professor of Analytical Chemistry at Helsinki University, Helsinki, Finland. She is well recognized in the field of separation science. She serves as Editor of Journal of Chromatography A. Prof. Riekkola is one of the leaders in chromatography with a large number of publications.

Affiliations and expertise

Professor, Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Finland