Laboratory Practices in Microbiology
- 1st Edition - February 6, 2021
- Imprint: Academic Press
- Author: Osman Erkmen
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 1 0 1 7 - 0
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 0 0 1 5 - 7
Laboratory Practices in Microbiology provides updated insights on methods of isolation and cultivation, morphology of microorganisms, the determination of biochemical activitie… Read more
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Request a sales quoteLaboratory Practices in Microbiology provides updated insights on methods of isolation and cultivation, morphology of microorganisms, the determination of biochemical activities of microorganisms, and physical and chemical effects on microorganisms. Sections cover methods of preparation of media and their sterilization, microorganisms in environment, aseptic techniques, pure culture techniques, preservation of cultures, morphological characteristics of microorganisms, wet-mount and hanging-drop techniques, different staining techniques, cultural and biochemical characteristics of bacteria, antimicrobial effects of agents on microorganisms, hand scrubbing in the removal of microorganisms, characteristics of fungi, uses of bacteriophages in different applications, and more.
Applications are designed to be common, complete with equipment, minimal expense and quick to the markets. Images are added to applications, helping readers better follow the expressions and make them more understandable. This is an essential book for students and researchers in microbiology, the health sciences, food engineering and technology, and medicine, as well as anyone working in a laboratory setting with microorganisms.
- Gives complete explanations for all steps in experiments, thus helping readers easily understand experimental procedures
- Includes certain subjects that tend to be disregarded in other microbiology laboratory books, including microorganisms in the environment, pure culture methods, wet-mount and hanging drop methods, biochemical characteristics of microorganisms, osmotic pressure effects on microorganisms, antiseptic and disinfectants effects on microorganisms, and more
- Provides groupings and characterizations of microorganisms
- Functions as a representative reference book for the field of microbiology in the laboratory
Students and teachers in the field of microbiology, biology and food science. Students, researchers, teachers, in laboratory techniques. It can be used by researchers and microbial analysis laboratories in the industry, biology, medicine and health sciences
- Cover image
- Title page
- Table of Contents
- Copyright
- About the author
- Preface
- Acknowledgments
- Introduction to the student
- Materials to be provided by student
- General laboratory rules
- Laboratory safety
- Section I: Microbiological media and cultural techniques
- Introduction
- Practice 1: Preparation of media and sterilization techniques
- Abstract
- 1.1: Theoretical information
- 1.2: Techniques in the preparation of media
- Practice 2: Microorganisms in environment
- Abstract
- 2.1: Theoretical information
- 2.2: Microbial detection techniques from environment
- Practice 3: Aseptic techniques
- Abstracts
- 3.1: Theoretical information
- 3.2: Types of disinfectants and antiseptics
- 3.3: Aseptic transfer techniques in microbiological researches
- Practice 4: Pure culture techniques
- Abstract
- 4.1: Theoretical information
- 4.2: Plate culture techniques
- Practice 5: Culture preservation techniques
- Abstract
- 5.1: Theoretical information
- 5.2: Preservation techniques
- Section II: Techniques on morphological examination of bacteria
- Introduction
- Practice 6: Microscope for study of microorganisms
- Abstract
- 6.1: Light microscope
- 6.2: Uses of microscope
- Practice 7: Microbial cell measurement technique
- Abstract
- 7.1: Theoretical information
- 7.2: Cell measurement technique
- Practice 8: Wet-mount and hanging-drop techniques
- Abstract
- 8.1: Theoretical information
- 8.2: Microscopic techniques
- Practice 9: Smear preparation and simple staining techniques
- Abstract
- 9.1: Theoretical information
- 9.2: Staining techniques
- Practice 10: Gram staining technique
- Abstract
- 10.1: Theoretical information
- 10.2: Gram staining
- Practice 11: Bacterial endospore staining techniques
- Abstract
- 11.1: Theoretical information
- 11.2: Endospore staining techniques
- Practice 12: Bacterial capsule staining techniques
- Abstract
- 12.1: Theoretical information
- 12.2: Capsule staining techniques
- Practice 13: Motility tests
- Abstract
- 13.1: Theoretical information
- 13.2: Motility indication techniques
- Section III: Biochemical and cultural characteristics of microorganisms
- Introduction
- Practice 14: Cultural characteristics of bacteria
- Abstract
- 14.1: Theoretical information
- 14.2: Techniques used in the indication of cultural characteristics
- Practice 15: Oxidation and fermentation
- Abstract
- 15.1: Theoretical information
- 15.2: Fermentation and oxidation tests
- Practice 16: Hydrolytic reactions
- Abstract
- 16.1: Theoretical information
- 16.2: Hydrolytic reactions on different substrates
- 16.3: Hydrolytic reaction tests
- Section IV: Chemical and physical effects on microorganisms
- Introduction
- Practice 17: Antiseptic and disinfectant effects on microorganisms
- Abstract
- 17.1: Theoretical information
- 17.2: Antiseptics and disinfectants antimicrobial sensitivity test techniques
- Practice 18: Antibiotic sensitivity test technique
- Abstract
- 18.1: Theoretical information
- 18.2: Kirby-Bauer antibiotic sensitivity test technique
- Practice 19: Effect of hand scrubbing on removal of microorganisms
- Abstract
- 19.1: Theoretical information
- 19.2: Hand scrubbing
- Practice 20: Temperature effects on microorganisms
- Abstract
- 20.1: Theoretical information
- 20.2: Indication of temperature effects on microorganisms
- Practice 21: Osmotic pressure and pH effects on microorganisms
- Abstract
- 21.1: Theoretical information
- 21.2: Effects of osmotic pressure and pH on microorganisms
- Practice 22: Ultraviolet light effects on microorganisms
- Abstract
- 22.1: Theoretical information
- 22.2: Ultraviolet light application
- Practice 23: Oxygen effects on microorganisms
- Abstract
- 23.1: Theoretical information
- 23.2: Determination of oxygen effects on microorganisms
- Section V: Fungi and bacteriophages
- Introduction
- Practice 24: Morphological characteristics of yeasts and molds
- Abstract
- 24.1: Theoretical information
- 24.2: Morphological characteristics fungi
- Practice 25: Isolation, enumeration, and uses of bacteriophages
- Abstract
- 25.1: Theoretical information
- 25.2: Uses of bacteriophages
- 25.3: Life cycle of bacteriophages
- 25.4: Bacteriophage culturing techniques
- Appendix: Preparation of culture media, stains, and solutions
- A.1: Preparation of culture media
- A.2: Preparation of stains
- A.3: Preparation of solutions
- Bibliography
- Index
- Edition: 1
- Published: February 6, 2021
- Imprint: Academic Press
- No. of pages: 292
- Language: English
- Paperback ISBN: 9780323910170
- eBook ISBN: 9780323900157
OE
Osman Erkmen
Osman Erkmen is professor of food microbiology in the Department of Food Engineering, Gaziantep University. Gaziantep, Turkey. He started his career as a research assistant at the Department of Food Engineering in 1985 and later became an assistant professor in 1994 and associate professor of food microbiology in 1999. He has been working as a professor in this department since 2004, where he teaches courses in general microbiology, food microbiology, food sanitation, and food toxicology. His research focuses on the uses of nonthermal processes and natural antimicrobials in food preservation; the production of fermented foods; the microbial production of lycopene, thiamin, alcohol, and citric acid from industrial wastes; and microbial inactivation and modeling. He studies the combined effect of nonthermal processes, natural antimicrobials in the destruction of microbial cells and spores, and its application in food preservation, as well as characteristics of white and red wines production from Gaziantep grapes. Professor Erkmen has published over 150 research articles, reviews, book chapters, proceeding articles, and popular articles, edited two books, and authored three books in the fields of food microbiology, general microbiology, food toxicology and food sanitation, with more than 3,500 citations. He has more than 10 patents, organized more than 20 international scientific symposiums, and participated in more than 65 international symposiums.
Affiliations and expertise
Department of Food Engineering, Faculty of Engineering, University of Gaziantep, Gaziantep, TurkeyRead Laboratory Practices in Microbiology on ScienceDirect