
Laboratory Methods in Microbiology and Molecular Biology
Methods in Molecular Microbiology
- 1st Edition - June 13, 2023
- Imprint: Academic Press
- Authors: Richa Salwan, Vivek Sharma
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 5 0 7 8 - 7
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 5 0 7 9 - 4
Laboratory Methods in Microbiology and Molecular Biology describes various microbiological, biochemical, and molecular methods employed for the characterization, identific… Read more

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Request a sales quoteLaboratory Methods in Microbiology and Molecular Biology describes various microbiological, biochemical, and molecular methods employed for the characterization, identification, and analysis of actinomycetes, bacteria and fungi. The book details general guidelines, expectations, and responsibilities for good lab practices and consists of chapters that covers basic microbiological, physiological, biochemical, and molecular aspects as well as in silico analysis using various bioinformatic tools. Other topics in the book include how to make solutions, microscopy and imaging of microorganisms, sero-diagnostics, and basic concepts of phylogeny, physiology, biotechnology, soil, food, and environmental microbiology while working in laboratory. Laboratory Methods in Microbiology and Molecular Biology is an informative update to current practices and future perspectives for the field of microbial biotechnology. It aims to facilitate professors, researchers, and graduate students in monitoring the precision and accuracy of the qualitative and quantitative methods in their research.
- Involves various procedures in diverse disciplines, from microbiology to genetics, molecular biology and biochemistry
- Lists the principles and facts underlying practical applications of bacteria and fungi which have prospects in various technologies
- Includes the questions ‘how’ and ‘why’ as an explanation for novice students and researchers to modify protocols
- Facilitates students, teachers and researchers to monitor the precision and accuracy of their qualitative and quantitative methods practically
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributor
- Authors' biographies
- Preface
- Acknowledgments
- Chapter 1. General guidelines, expectations, and responsibilities for inculcating good lab practices
- 1. Distributing/sharing lab responsibilities
- 2. Awareing students/researchers for research ethics/Authorship claims
- 3. Celebrations
- 4. Lab meetings
- 5. Reproducibility and data management
- 6. Scientific collection/work life balance
- 7. Handling reagents/Solutions and visiting fields
- 8. Laboratory safety
- 9. Personal safety
- 10. Preparing standard solutions
- Chapter 2. Preparations of laboratory solutions
- 1. Guidelines for preparing reagents, buffers, etc
- 2. General considerations for working with RNA
- 3. General terms and description for the preparation of reagents
- 4. Selection and preparation of buffer solution
- Chapter 3. Basic concepts of microbiology
- 1. Aim: preparation of culture media for the routine cultivation of bacteria
- 2. Aim: general techniques for the isolation and cultivation of bacteria
- 3. Aim: to preserve bacterial and fungal cultures by using different methods
- Chapter 4. Microscopy and imaging analysis of microorganisms
- 1. Aim: to prepare wet mount slide of the given sample
- 2. Aim: to observe motility of bacteria using hanging drop experiment
- 3. Aim: preparation of bacterial smear
- 4. Aim: to prepare a slide for simple staining
- 5. Aim: to perform negative staining of bacteria
- 6. Aim: to prepare a slide for Gram's staining
- 7. Aim: capsule staining by anthony staining method
- 8. Aim: to perform capsule staining of the bacterial culture
- 9. Aim: to stain flagella of a bacterial culture
- 10. Aim: to perform spore staining of the given culture
- 11. Aim: perform spore staining using Dorner's method
- 12. Aim: to stain the nuclear material and study the detailed morphology of the nucleus
- 13. Aim: to perform lactophenol cotton blue staining of the give fungal culture
- 14. Aim: to perform staining of a given actinomycetes culture
- 15. Aim: to perform acid-fast staining of a given culture
- Chapter 5. Characterization of microbes using molecular methods
- 1. Morphological characterization using Biolog
- 2. Microbial identification based on FAME
- 3. Electron microscopy
- 4. Molecular characterization of bacteria
- Chapter 6. Soil microbiology
- 1. Aim: Isolation of Rhizobium from the given sample
- 2. Aim: To isolate Actinomycetes from the given soil sample
- 3. Aim: Isolation of Azotobacter from soil
- 4. Aim: To estimate soil microbial biomass by Chloroform fumigation extraction method
- 5. Aim: To estimate the quantity of various enzymes in soil samples
- 6. Aim: To study the effect of pH on the growth of microbes
- 7. Aim: To study the effect of temperature on growth of microbes
- 8. Aim: To study the effect of salt concentration on growth of microbes
- 9. Aim: To perform dual culture assay or direct confrontation assay
- Chapter 7. Basic experiments in food microbiology
- 1. Aim: Evaluation of milk quality using methylene blue reduction test (MBRT) assay
- 2. Aim: Isolation of lactic acid bacteria
- 3. Aim: To prepare yogurt under laboratory conditions
- 4. Aim: Preparation of bread from wheat flour
- 5. Aim: To prepare alcohol from grape juice under laboratory conditions
- 6. Aim: To prepare sauerkraut from cabbage
- 7. Aim: To prepare cheese under laboratory conditions
- Chapter 8. Basic experiments in microbial biochemistry
- 1. Aim: Qualitative screening of bacteria for amylase production
- 2. Aim: Qualitative screening of bacteria for lipase production
- 3. Aim: Qualitative screening of bacteria for protease production
- 4. Aim: Qualitative screening of bacteria for indole production
- 5. Aim: To perform methyl red (MR) test for detection of coliforms
- 6. Aim: To perform Voges-Proskauer test
- 7. Aim: Assessment of bacteria for utilization of sodium citrate as carbon source
- 8. Aim: To perform catalase test
- 9. Aim: Qualitative assessment of oxidase production
- 10. Aim: Assessment of carbohydrate fermentation
- 11. Aim: To perform oxidation-fermentation test
- 12. Aim: To perform nitrate reduction test
- 13. Aim: Qualitative assessment of urea hydrolysis
- 14. Aim: To perform triple sugar iron agar (TSIA) test
- Chapter 9. Analytical techniques in microbial genomics
- 1. Isolation of nucleic acid (DNA and RNA) from microorganisms
- 2. Analysis of nucleic acid (DNA and RNA) from microorganisms
- Chapter 10. Analytical techniques in molecular biology-proteomics
- 1. Basics of protein electrophoresis
- 2. SDS-PAGE
- 3. Consumables
- 4. Equipments
- 5. SDS-PAGE staining using Coomassie R250 or G250
- 6. Silver staining
- 7. Chemicals
- 8. Equipments and per-requisite
- 9. Method
- 10. Fluorescent silver staining
- 11. Chemicals
- 12. Equipments and per-requisite
- 13. Method
- Chapter 11. Analytical techniques in proteomics
- 1. Aim: Isolation and characterization of extracellular protein
- 2. Protein dialysis and ultrafiltration
- 3. Protein fractionation and desalting using gel filtration chromatography
- 4. Use of bioinformatics in planning a protein purification
- Chapter 12. Expression of recombinant proteins in prokaryotic host
- 1. Aim: Cloning and transformation of gene of interest in expression vector
- 2. Background of pET vector system and expression host
- 3. Equipment and reagents for cloning and expression of genes
- 4. Protocol
- 5. Optimization of insert: vector molar ratios
- Chapter 13. Sero-diagnostics in microbiology
- 1. Enzyme-linked immunosorbent assay (ELISA) and variants of ELISA
- 2. Western blotting
- 3. Immunoprecipitation/agglutination assay using blood sample
- 4. To perform ouchterlony double diffusion or passive double immunodiffusion
- 5. To perform radial immunodiffusion assay
- Chapter 14. Basic tools for next-generation DNA sequencing of bacterial genome
- 1. Background
- 2. Protocol
- 3. Reagents for illumina
- 4. Equipments
- 5. Protocol
- 6. Bioinformatic analyses
- Index
- Edition: 1
- Published: June 13, 2023
- Imprint: Academic Press
- No. of pages: 206
- Language: English
- Paperback ISBN: 9780323950787
- eBook ISBN: 9780323950794
RS
Richa Salwan
VS