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Laboratory Methods in Enzymology: RNA
1st Edition - September 11, 2013
Author: Jon Lorsch
eBook ISBN:
9 7 8 - 0 - 1 2 - 4 2 0 0 5 1 - 7
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and… Read more
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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.
In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.
- Indispensable tool for the researcher
- Carefully written and edited by experts to contain step-by-step protocols
- In this volume we have brought together a number of core protocols concentrating on RNA
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
Computational Prediction of RNA Secondary Structure
Reverse-transcription PCR (RT-PCR)
Northern blotting
Explanatory Chapter: Nuclease Protection Assays
In Vitro Transcription from Plasmid or PCR-amplified DNA
Measurement of In Vivo RNA Synthesis Rates
Method for measuring mRNA decay rate in Saccharomyces cerevisiae
Analysis of polysomes from bacteria
Polysome Profile Analysis – Yeast
Polysome analysis of mammalian cells
Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae
Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates
Circularized RT-PCR (cRT-PCR): analysis of the 5′ ends, 3′; ends, and poly(A) tails of RNA
RNA Radiolabeling
Fluorescently Labeling Synthetic RNAs
Analysis of RNA by analytical polyacrylamide gel electrophoresis
RNA purification by preparative polyacrylamide gel electrophoresis
Explanatory Chapter: Nucleic Acid Concentration Determination
RNA purification – precipitation methods
Reverse Transcriptase Dideoxy Sequencing of RNA
RNA Structure Experimental Analysis – Chemical Modification
Structural Analysis of RNA Backbone Using In-Line Probing
- No. of pages: 472
- Language: English
- Published: September 11, 2013
- Imprint: Academic Press
- eBook ISBN: 9780124200517
JL
Jon Lorsch
Affiliations and expertise
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA