Ion-Exchange Chromatography and Related Techniques

1st Edition - November 15, 2023
Editors: Pavel Nesterenko, Colin Poole, Yan Sun
Language: English
Paperback ISBN:
9 7 8 - 0 - 4 4 3 - 1 5 3 6 9 - 3
eBook ISBN:
9 7 8 - 0 - 4 4 3 - 1 5 3 7 0 - 9

Ion-Exchange Chromatography and Related Techniques defines the current state-of-the-art in ion-exchange chromatography and related techniques and their implementation in labora… Read more

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Ion-Exchange Chromatography and Related Techniques defines the current state-of-the-art in ion-exchange chromatography and related techniques and their implementation in laboratory and industrial practice. This book provides a compact source of information to facilitate the transfer of knowledge and experience acquired by separation science specialists to colleagues from diverse backgrounds who need to acquire fundamental and practical information to facilitate progress in research and management functions reliant on information acquired by separation. Individual chapters written by recognized experts lending credibility to the work will allow this book to serve as a high value reference source of current information for analytical and biopharmaceutical chemists.

Cover image
Title page
Table of Contents
Copyright
Contributors
Chapter 1 Concepts and milestones in the development of ion-exchange chromatography
Abstract
1 Introduction
2 Fundamentals
3 Column chromatography
4 Large-scale ion-exchange separations
5 Landmark developments in biotechnology for downstream processing using ion-exchange chromatography
References
Chapter 2 Equilibria and kinetics of ion-exchange of biopolymers
Abstract
1 Introduction
2 Dynamic models
3 Kinetic equations of adsorption-desorption rate
4 Adsorption-desorption equilibria: Isotherm equations
5 Causes of misinterpretation of the elution data
6 Procedure for design of IEX process
References
Chapter 3 Stationary phases for ion separations
Abstract
1 Ion-exchange terminology
2 Classification of ion-exchangers
References
Chapter 4 Stationary phases for the separation of biopolymers by ion-exchange chromatography
Abstract
1 Introduction
2 Uniform agarose-based ion-exchange chromatographic media
3 Gigaporous ion-exchange chromatographic media
4 Other ion-exchange stationary phases for bioseparations
5 Summary and outlook
References
Chapter 5 Ion-exchange separations of biomacromolecules on grafted and surface-modified polymers
Abstract
1 Introduction
2 Stationary phases
3 Adsorption and uptake theory
4 Applications
References
Chapter 6 Extraction chromatography of actinides
Abstract
1 Introduction
2 Extractants for actinide separation
3 Ligand impregnated resins for actinides
4 Room temperature ionic liquids in extraction chromatography
5 Ligand grafted resins for actinides
6 Composite beads for extraction chromatography
7 Perspectives
References
Chapter 7 Ion-exchange membrane chromatography
Abstract
1 Introduction
2 Transport phenomena in membrane chromatography
3 Module design
4 Promising ion-exchange membranes for bioseparations
5 Conclusions
References
Chapter 8 Ion-exclusion chromatography
Abstract
1 Principle
2 Apparatus
3 Ion-exchange resin columns used in ICE
4 Eluent conditions
5 Detection methods
6 Separations of nonionized substances
7 Separation of ammonium and amines
8 Vacancy ion-exclusion chromatography
9 Ion-exclusion/cation-exchange chromatography
10 Ion-exclusion/anion-exchange chromatography
References
Chapter 9 Chelation ion chromatography
Abstract
1 Introduction
2 Theoretical aspects of complexation in liquid chromatography
3 Ion-exchange chromatography with the complex formation in the mobile phase
4 Chelating phases for ion-exchange chromatography
5 Application areas of chelating ion-exchangers
References
Chapter 10 Displacement chromatography with ion-exchangers
Abstract
1 Principles of displacement chromatography
2 Ion-exchange displacers
3 Applications of ion-exchange displacement chromatography
4 Prospects and outlook
References
Chapter 11 Instrumentation for ion chromatography
Abstract
1 Solvent delivery systems for IC applications
2 Detectors for IC
3 Injection system
4 Column oven
5 Column hardware
References
Chapter 12 Instrument platforms for large-scale ion-exchange separations of biomolecules
Abstract
1 Introduction
2 Chromatography columns
3 Ion exchange matrices
4 Chromatography equipment
5 Scale-up of ion-exchange processes
References
Chapter 13 Method development for large molecules IEX separations
Abstract
1 Introduction
2 Column, stationary phase, and instrumentation considerations
3 Elution modes
4 IEX mode for nucleic acid separations
5 On-off mechanism of retention
6 Need for IEX platform methods
7 Systematic method development
8 Practical advice for systematic method optimization
9 Perspectives
Declaration of competing interest
References
Chapter 14 Sample preparation for ion-exchange separations
Abstract
1 Introduction
2 Liquid-phase extraction
3 Solid-phase extraction
4 Membrane-based extraction
References
Chapter 15 Separation of ions by ion chromatography
Abstract
1 Anion-exchange chromatography
2 Cation-exchange chromatography
References
Chapter 16 Applications of ion chromatography in environmental analysis
Abstract
1 Introduction
2 Ion chromatography and related techniques
3 IC development perspectives
References
Chapter 17 Applications of ion chromatography in food analysis
Abstract
1 Introduction
2 Inorganic anions
3 Inorganic cations
4 Carbohydrates
5 Organic acids
6 Food safety issues
References
Chapter 18 Separation of saccharides by ion-exchange chromatography
Abstract
1 Introduction
2 Separation of saccharides by HPAEC
3 Separation of saccharides by ligand exchange chromatography
4 Ion exclusion chromatography
5 Ion-pair chromatography
References
Chapter 19 Separation of oligonucleotides by ion-exchange chromatography
Abstract
1 Introduction
2 Column packings for the ion-exchange separations
3 Nucleotides and sugar nucleotides
4 Oligonucleotides
5 Phosphorothioated oligonucleotides
6 Mixed mode stationary phases
7 Multidimensional chromatography
8 Purification of oligonucleotides
References
Chapter 20 Separation of oligonucleotides by ion-exchange and ion-pair chromatography
Abstract
1 Introduction
2 Types of oligonucleotides
3 Endogenous RNAs
4 Therapeutic oligonucleotides
5 Charged-based separations of oligonucleotides
6 Nonspecific adsorption
7 Sample preparation for oligonucleotides
8 Liquid chromatography-mass spectrometry of oligonucleotides
9 Selected applications of ion-exchange and ion-pair chromatography to the determination of oligonucleotides
10 Future directions
References
Chapter 21 Separation of proteins by ion-exchange chromatography
Abstract
1 Introduction
2 Adsorption: Principles and models
3 Evolution of resins
4 Investigational approaches
5 Common modes of operation
6 Perspectives
References
Chapter 22 Separation of proteins by mixed-mode chromatography
Abstract
1 Introduction
2 Mixed mode ligands
3 Effect of salt and pH on mixed-mode chromatography
4 The third dimension
5 High-throughput screening of mixed-mode resins
6 Monoclonal antibody purification
7 Conclusion
References
Chapter 23 Process modeling of protein separations by ion-exchange chromatography
Abstract
1 Introduction
2 Mechanistic modeling of chromatography
3 Plate height and related variables in isocratic elution
4 Resolution Rs in isocratic elution
5 Ion-exchange equilibria
6 Linear gradient elution
7 Applications of the mechanistic models and the simplified methods
8 Capture process design
References
Chapter 24 Applications of ion-exchange chromatography for the purification of antibodies
Abstract
Acknowledgment
1 Antibodies: Introduction, recombinant expression, manufacturing, and purification
2 Cation-exchange chromatography of antibodies
3 Anion-exchange chromatography of antibodies
4 Commercial ion-exchange resins
5 Scale-up and scale-down of ion-exchange chromatography
6 Tips and tricks
References
Chapter 25 Conformational changes of biomolecules in ion-exchange chromatography
Abstract
1 Introduction
2 Mechanisms
3 Detection of protein conformational changes
4 Key contributing factors
References
Chapter 26 Continuous ion-exchange chromatography for protein polishing and enrichment
Abstract
1 Introduction
2 Limits of batch chromatography
3 Continuous chromatography for protein purification
4 Process design for continuous purification
5 Applications for protein polishing using ion-exchange
6 Conclusion
References
Chapter 27 Separation of bio-particles by ion-exchange chromatography
Abstract
Acknowledgment
1 Introduction
2 The principles of separating bio-particles by IEC
3 Challenges and coping strategies for separating bio-particles by IEC
4 Design of IEC-based bio-particle separations
5 Future prospects
References
Chapter 28 Virus removal in bioprocessing using charged media
Abstract
1 Introduction
2 Virus physicochemical properties
3 Protein interference
4 Chromatography format
5 Cation exchange and multimodal ligands
6 Conclusions
References
Chapter 29 Role of temperature in ion-exchange processes of separation and purification
Abstract
1 Influence of temperature on the stability of ion-exchange resins
2 Influence of temperature on the kinetics and dynamics of ion exchange
3 Influence of temperature on the ion-exchange equilibrium
4 The role of temperature in traditional ion-exchange processes using auxiliary reagents
References
Index

Pavel Nesterenko

Prof. Pavel Nesterenko holds a position of Leading Researcher in the Laboratory of Adsorption and Gas Chromatography at the Department of Chemistry M.V. Lomonosov Moscow State University. Received MSc (1979) in petroleum chemistry and organic catalysis, PhD (1984) and DSc degrees (2000) in analytical chemistry from M.V. Lomonosov Moscow State University (Moscow, Russia). Senior Researcher (1988), Leading Researcher (2001) and Professor of Analytical Chemistry (2003). In 2006-2018 was awarded Quantum Leap Professor and New Star Professor positions at Australian Centre for Research on Separation Science (ACROSS) and worked at the University of Tasmania, Hobart, Australia. Author of more than 380 scientific publications including 4 books, 14 Chapters in books, 350 regular papers and 13 patents. Research interests are associated with development and investigation of new advanced materials with focus on their selectivity and efficiency for various separation and detection technologies.

Affiliations and expertise

Leading Researcher, Moscow State University, Russia, Laboratory of Adsorption and Gas Chromatography, Chemistry Department Lomonosov Moscow State University, Moscow, Russia

Colin Poole

Professor Colin Poole is internationally known in the field of thin-layer chromatography and is an editor of the Journal of Chromatography and former editor of the Journal of Planar Chromatography – Modern TLC. He has authored several books on chromatography, recent examples being The Essence of Chromatography published by Elsevier (2003), and Gas Chromatography published by Elsevier (2012). He is the author of approximately 400 research articles, many of which deal with thin-layer chromatography, and is co-chair of the biennial “International Symposium on High-Performance Thin-Layer Chromatography”.

Affiliations and expertise

Department of Chemistry, Wayne State University, Detroit, MI, USA

Yan Sun

Professor Yan Sun received Doctor of Engineering Degree in the University of Tokyo in 1990. He started his teaching and research career in Tianjin University (TJU) in 1991 and became a full Professor in 1993. He is now a Chair Professor of TJU. In 2000, he was appointed to the Cheung Kong Professorship by the Ministry of Education (MOE) of China. In 2011, he received the Young Asian Biochemical Engineers’ Community Award from the Asian Federation of Biotechnology, and during 2002 to 2012 he received three academic research awards on bioseparations in China. His research interests include bioseparations and biocatalysis. He is an Editor of the Journal Chromatography A, an Associate Editor of Biochemical Engineering Journal, and an Associate Editor of Chinese Journal of Chemical Engineering. His work has produced over 400 research papers published in peer-reviewed international journals and one book (Bioseparations Engineering, in Chinese).

Affiliations and expertise

Chair Professor and Cheung Kong Professor, Biochemical Engineering, Tianjin University, Tianjin, China