Ion Channels: Channel Biochemistry, Reconstitution, and Function

Preface

1. Correlating ion channel structure and function

2. TRPV3 Expression and Purification for Structure Determination by Cryo-EM

3. Methods to study phosphoinositide regulation of ion channels

4. Recombinant Expression and Purification of Pentameric Ligand-Gated Ion Channels for Cryo-EM Structural Studies

Sudha Chakarpani

5. Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography

6. Structural Dynamics of Channels and Transporters by High-Speed Atomic Force Microscopy

7. Crosslinking for gating motions in GluRs

8. Single molecule FRET methodology for investigating glutamate receptors

9. Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues

10. A quantitative flux assay for the study of Cl- channels and transporters

11. Ion channels reconstitution in lipid bilayers

12. Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers

13. The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture