
Introductory Microbiology Lab Skills and Techniques in Food Science
- 1st Edition - November 2, 2021
- Imprint: Academic Press
- Authors: Cangliang Shen, Yifan Zhang
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 8 2 1 6 7 8 - 1
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 3 2 4 3 - 9
Introductory Microbiology Lab Skills and Techniques in Food Science covers topics on isolation, identification, numeration and observation of microorganisms, biochemistry tes… Read more

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Request a sales quoteIntroductory Microbiology Lab Skills and Techniques in Food Science covers topics on isolation, identification, numeration and observation of microorganisms, biochemistry tests, case studies, clinical lab tasks, and basic applied microbiology. The book is written technically with figures and photos showing details of every lab procedure. This is a resource that is skills-based focusing on lab technique training. It is introductory in nature, but encourages critical thinking based on real case studies of what happens in labs every day and includes self-evaluation learning questions after each lab section. This is an excellent guide for anyone who needs to understand how to apply microbiology to the lab in a practical setting.
- Presents step-by-step lab procedures with photos in lab setting.
- Includes case studies of microorganism causing infectious disease.
- Provides clinical microbial lab tasks to mimic real-life situations applicable to industry.
Anyone in industry and academia needing a basic understanding or refresher of microbial techniques in the lab. A supplement material for food microbiology lab and other microbial lab courses
- Cover Image
- Title Page
- Copyright
- Dedication
- Table of Contents
- Contributors
- Preface
- Acknowledgments
- Chapter 1 Introduction of microbial and chemical safety and lab notebook record
- Abstract
- Chapter Outline
- 1.1 Lab safety rules
- Chapter 2 Practice of bright-field microscope
- Abstract
- Chapter Outline
- 2.1 Materials
- 2.2 Introduction
- 2.3 Terminologies of bright-field microscope
- 2.4 Procedure of focusing prepared slides
- 2.5 After class question
- Chapter 3 Preparation of smears and simple stain practice
- Abstract
- Chapter Outline
- 3.1 Materials
- 3.2 Introduction
- 3.3 Morphology of bacterial cells in this lab practice
- 3.4 Procedure
- 3.5 After class questions
- Chapter 4 Gram-stain practice
- Abstract
- Chapter Outline
- 4.1 Materials
- 4.2 Introduction
- 4.3 Gram-stain results of bacterial cells in this lab practice
- 4.4 Procedure
- 4.5 After class questions
- Chapter 5 Acid-fast stain practice
- Abstract
- Chapter Outline
- 5.1 Materials
- 5.2 Introduction
- 5.3 Clinical diagnose procedure
- 5.4 Procedure
- 5.5 After class questions
- Chapter 6 Endospore stain practice
- Abstract
- Chapter Outline
- 6.1 Materials
- 6.2 Introduction
- 6.3 Procedure
- 6.4 After class questions
- Chapter 7 Aseptic technique-bacteria transfer and streak plating
- Abstract
- Chapter Outline
- 7.1 Materials
- 7.2 Introduction
- 7.3 Procedure
- 7.4 After class questions
- Chapter 8 Lab practice test 1 – identify unknown bacteria using Gram-stain
- Abstract
- Chapter Outline
- 8.1 Introduction
- 8.2 Procedure
- 8.3 Lab report (1–2 pages, typed)
- Chapter 9 Introduction of bacteria medium, nutritional requirements (synthetic and complex media), selective & differential media (MacConkey, mannitol salt, blood agar)
- Abstract
- Chapter Outline
- 9.1 Introduction
- 9.2 MacConkey agar
- 9.3 Mannitol salt agar
- 9.4 Blood agar
- 9.5 Procedure
- 9.6 After class questions
- Chapter 10 Lab practice test 2 – dilution technology and quiz
- Abstract
- Chapter Outline
- 10.1 Introduction
- 10.2 Dilution technique figures (assume 0.1 mL of each dilution tube adding onto agar plates)
- 10.3 Procedure of calculating bacterial concentration
- 10.4 After class questions
- Chapter 11 Numeration bacteria population by pour plating
- Abstract
- Chapter Outline
- 11.1 Materials
- 11.2 Introduction
- 11.3 Dilution schedule as shown in Fig. 11.1
- 11.4 After class question
- Chapter 12 Numeration bacteria population by spread plating
- Abstract
- Chapter Outline
- 12.1 Materials
- 12.2 Introduction
- 12.3 Dilution schedule as shown in Fig. 12.1
- 12.4 After class question
- Chapter 13 External conditions for bacterial growth (temperature, pH, oxygen, osmotic)
- Abstract
- Chapter Outline
- 13.1 Materials
- 13.2 Introduction
- 13.3 Exercise 1. Optimal growth requirements (temperature)
- 13.4 Exercise 2. Physiological effects of temperature
- 13.5 Exercise 3. Cultivation of aerobic, facultative, and anaerobic bacteria in thioglycolate broth
- 13.6 Exercise 4. Impact of osmotic pressure on bacterial growth
- 13.7 Exercise 5. Impact of pH on bacterial growth
- 13.8 After class questions
- Chapter 14 Cultivation of anaerobic bacteria
- Abstract
- Chapter Outline
- 14.1 Introduction
- 14.2 Procedure
- 14.3 After class questions
- Chapter 15 Biochemistry test of bacteria-1 (urease test, carbohydrate fermentation, catalase test, oxidase test)
- Abstract
- Chapter Outline
- 15.1 Exercise 1. Urease test: One urea broth tube/student
- 15.2 Exercise 2. Fermentation of carbohydrates: One of each carbohydrate fermentation tube/student
- 15.3 Exercise 3. Catalase test: One glass slide/student
- 15.4 Exercise 4. Oxidase test
- 15.5 After class questions
- Chapter 16 Biochemistry test of bacteria-2 (nitrate reduction, decarboxylation, & deamination of amino acids, IMViC test, hydrogen sulfide, motility, and indole test)
- Abstract
- Chapter Outline
- 16.1 Exercise 1. Sulfate indole motility test: One sulfate indole motility medium tube/student
- 16.2 Exercise 2. Phenylalanine deaminase test
- 16.3 Exercise 3. Methyl Red Voges-Proskauer (MRVP) testing: Two MRVP tubes/group
- 16.4 Exercise 4. Nitrate reduction test: One tube of nitrate broth/student
- 16.5 After class questions
- Chapter 17 Biochemistry test of bacteria-3 (API20e and Enterotube II)
- Abstract
- Chapter Outline
- 17.1 Materials
- 17.2 Introduction
- 17.3 Exercise 1. Use Enterobute II to identify Escherichia coli
- 17.4 Exercise 2. Use API 20e
- 17.5 Validation after 24 h at 35°C
- 17.6 After class questions
- Chapter 18 Extracellular enzymatic activities of microorganisms
- Abstract
- Chapter Outline
- 18.1 Materials
- 18.2 Exercise 1. Lipase test: One spirit blue agar plate/student
- 18.3 Exercise 2. Starch Hydrolysis: One starch agar plate/student
- 18.4 Exercise 3. Protein hydrolysis (protease test): One skim milk agar plate/student
- 18.5 After class questions
- Chapter 19 Case study 1 (bacteria cause upper respiratory tract diseases)
- Abstract
- Chapter Outline
- 19.1 Introduction
- 19.2 Lancefield groups
- 19.3 Streptococci
- 19.4 Staphylococci
- 19.5 Procedure
- Chapter 20 Clinical lab practice - urine sample bacteria isolation and numeration
- Abstract
- Chapter Outline
- 20.1 Introduction
- 20.2 Exercise 1. Dip stick urine test
- 20.3 Exercise 2. Qualitative test of urine samples
- 20.4 Exercise 3. Quantitative test of urine samples
- 20.5 After class questions
- Chapter 21 Case study 2 (bacteria cause intestinal tract diseases)
- Abstract
- Chapter Outline
- 21.1 Introduction
- 21.2 Clostridium difficile
- 21.3 Clostridium botulinum
- 21.4 Clostridium tetani
- 21.5 Clostridium perfringens
- 21.6 Klebsiella pneumoniae
- 21.7 Enterococcus
- 21.8 Exercise 1
- Chapter 22 Isolation of phage and plaque forming units determination
- Abstract
- Chapter Outline
- 22.1 Warm-up questions
- 22.2 Objectives
- 22.3 Major materials
- 22.4 Introduction
- 22.5 Lab practice procedures
- 22.6 After class questions/readings
- Chapter 23 Antibiotic susceptibility testing and evaluation of antiseptics/disinfectants
- Abstract
- Chapter Outline
- 23.1 Materials
- 23.2 Introduction
- 23.3 Exercise 1. The Kirby-Bauer disk diffusion assay
- 23.4 Exercise 2. Evaluation of antimicrobials (filter paper disk method)
- 23.5 After class questions/readings
- Chapter 24 Microbial counts of food product
- Abstract
- Chapter Outline
- 24.1 Materials
- 24.2 Introduction
- 24.3 Exercise 1. Bacterial count on food, spread plate technique
- 24.4 Procedures for preparing food samples before analyzing
- 24.5 After class questions
- Chapter 25 Total plate counts & coliform counts of pond water
- Abstract
- Chapter Outline
- 25.1 Materials
- 25.2 Introduction
- 25.3 Coliform vs fecal coliform
- 25.4 Exercise 1. The membrane filter method
- 25.5 Exercise 2. The petri-film method
- 25.6 After class questions
- Appendices
- Index
- Edition: 1
- Published: November 2, 2021
- Imprint: Academic Press
- No. of pages: 174
- Language: English
- Paperback ISBN: 9780128216781
- eBook ISBN: 9780128232439
CS
Cangliang Shen
YZ