Integrated Methods in Protein Biochemistry: Part B

1. Precise modification of native proteins, purification, and analysis of bioconjugates
Vishal Rai

2. NanoBiT-based methods to monitor the activation and modulation of RTKs
Andy Chevigne

3. The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling
Ajith Karunarathne

4. Structure and function of bacterial secretion system
Florian Schubot

5. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase
Renwick Dobson

6. Tools and protocols for probing protein sumoylation
Ram Kumar Mishra

7. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase
Thomas C. Brunold and Joshua Miller

8. DeGlyPHER: MS-based analysis of viral spike N-glycoforms
John Yates

9. Covalent protein painting: MS-based protein footprinting
John Yates

10. Characterization of GPCR signaling complexes using negative-staining electron microscopy
Arun K. Shukla

11. Probing protein misfolding and dissociation with free electron laser
Kazuhiro Nakamura and Hisashi Okumura

12. Optimized protocol for the characterization of Cas12a activities
Rakhi Rajan

13. Proximity proteomics for the identification and characterization of extracellular vesicles
Norihiro Kotani

14. Structural and Functional characterization of lytic polysaccharide monooxygenases
Vincent Eijsink

15. Characterization of RRE domain in RiPP biosynthesis
Douglas A. Mitchell

16. Structural and mechanistic insights into ferrous iron import and export systems
Aaron T. Smith

17. Testing anti-cancer drugs with holographic Incoherent light source quantitative phase imaging
Daniel Zicha

18. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry
Nobuaki Takemori

19. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins
Shawn Chen

20. Integrated studies on ageing-related proteins extending life span in yeast
Hiroyui Osada and Makoto Muroi

21. An optimized pipeline for global selenoproteome analysis
Chu Wang

22. High-throughput screening and evolution of carbohydrate-active enzymes
Shishir PS Chundawat

23. Regulation of enzymes with light using unnatural amino acids
Andrea Kneuttinger

24. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies
Sven Eyckerman

25. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics
Emily E. Weinert

26. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors
Nicholas Alexander Graham

27. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules
Joanna F. McGouran

28. Reconstitution of the MLL1 complex and probing methyltransferase activity
Yali Dou

29. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy
Geert van den Bogaart

30. Structural and functional analysis of mono-copper enzymes from fungal species
Vincent Eijsink