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Integrated Methods in Protein Biochemistry: Part A

  • 1st Edition, Volume 675 - October 9, 2022
  • Latest edition
  • Editor: Arun K. Shukla
  • Language: English

Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume pr… Read more

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Description

Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more.

Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.

Key features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Updated release includes the latest information on Integrated Methods in Protein Biochemistry

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of contents

1. DNA and protein engineering to create protein bioswitches with new functions
Stewart Loh

2. Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases
Susan Michaelis

3. An experimental protocol to study lipid transfer proteins
Haijia Yu

4. Synthesis of small heat shock proteins
Matthew Pratt

5. Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins
Guang-Yu Yang

6. An experimental protocol for glycoconjugate analysis
Yifa Zhou

7. Methods for proximity-based biotinylation combined with Mass Spectrometry
Ralph Kehlenbach

8. Synthetic antibody fragments as conformational sensors of protein activation and trafficking
Arun K. Shukla

9. Expression, purification, functional analysis and crystallization of Rag GTPase
Kuang Shen

10. Purification of bacterial transcription elongation complexes by photoreversible immobilization
Eric Strobel

11. Inhibition of c-Myc-MAX heterodimerization
Neha Garg

12. Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases
Markus C. Wahl

13. Phosphorylation and subcellular localization of human phospholipase A1
Atsushi Yamashita

14. Integrated methods in studying light-sensitive proteins
Lars-Oliver Essen

15. Structure and biochemistry of thermophilic protein
Ankan Banerjee

16. Using hydrogen/deuterium exchange mass spectrometry to probe protein structure
Gert Bange

17. Nanopore force spectroscopy to probe structural dynamics of proteins
Sonja Schmid

18. Structural analysis of knotted proteins
Shang-Te "Danny" Hsu

19. Mutation-induced structural changes in the SARC-CoV2 spike protein
Shang-Te "Danny" Hsu

20. Preparing metabolite capped RNA substrates using RNA polymerases
Jeremy G. Bird

Product details

  • Edition: 1
  • Latest edition
  • Volume: 675
  • Published: October 9, 2022
  • Language: English

About the editor

AS

Arun K. Shukla

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.
Affiliations and expertise
Indian Institute of Technology, Kanpur, India

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