Instrumental Thin-Layer Chromatography
- 2nd Edition - January 1, 2023
- Imprint: Elsevier
- Editor: Colin Poole
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 9 9 7 0 - 0
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 8 3 0 1 - 3
Instrumental Thin-Layer Chromatography, Second Edition offers a comprehensive source of authoritative information on all aspects of instrumental thin-layer chromatog… Read more
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Request a sales quoteInstrumental Thin-Layer Chromatography, Second Edition offers a comprehensive source of authoritative information on all aspects of instrumental thin-layer chromatography. The use of short, topic-focused chapters facilitates identifying information of immediate interest for familiar or emerging uses of thin-layer chromatography. The book gives those working in both academia and industry the opportunity to learn, refresh, or deepen their understanding of fundamental and instrumental aspects of thin-layer chromatography, as well as the tools to interpret and manage chromatographic data. The book serves as a practical consolidated guide to the selection of separation conditions and the use of auxiliary techniques.
This fully updated new edition restores the contemporary character of the book for those involved in advancing the technology, analyzing data produced, or applying the technique to new application areas. Some chapters have been consolidated to make room for topics not covered in the first edition, reflecting general changes in the field of thin-layer chromatography, especially in effects-directed detection, convenient interfaces for advanced spectroscopic detection, and greater automation possibilities. This book is a valuable reference for anyone who needs to acquire fundamental and practical information to facilitate progress in research and management functions utilizing information acquired by thin-layer chromatography.
- Features individual chapters written by recognized authoritative and visionary experts in the field
- Provides an overview and focused treatment of a single topic
- Provides tables and diagrams with commonly used data to facilitate practical work, comparison of results, and decision-making
- Places modern developments in the research literature into a general context not always apparent to inexperienced users of the technique
- Offers comprehensive updates to all chapters
- Includes new chapters on instrument platforms, effects-directed detection, data analysis tools, small-scale and microfluidic planar separation systems, and applications to the separation of amino acids and peptides, the analysis of saccharides and lipids, and forensic analysis
Analytical chemists, scientists, and researchers working in drug discovery, food and plant science, environmental science, and forensics; graduate students and instructors in these disciplines
- Cover image
- Title page
- Table of Contents
- Copyright
- List of contributors
- Section 1. Fundamentals and practice of thin-layer chromatography
- Chapter 1. Milestones, core concepts, and contrasts
- 1.1. Introduction
- 1.2. Milestones
- 1.3. Attributes of a planar format
- 1.4. Consequences of capillary-controlled flow
- 1.5. Solvent-strength gradients
- 1.6. Multidimensional separations
- 1.7. Conclusions
- Chapter 2. High-performance precoated stationary phases
- 2.1. Introduction
- 2.2. Inorganic oxide sorbents
- 2.3. Chemically bonded sorbents
- 2.4. Cellulose
- 2.5. Chiral sorbents
- 2.6. Ultrathin layers
- 2.7. Conclusions
- Chapter 3. Solvent selection and method development for thin-layer chromatography
- 3.1. Introduction
- 3.2. Problem definition
- 3.3. Mode selection
- 3.4. Mobile phase selection
- 3.5. General strategies for method development
- 3.6. Conclusions
- Chapter 4. Automated multiple development
- 4.1. Introduction
- 4.2. Applications
- 4.3. Future trends
- Chapter 5. Instrument platforms for high-performance thin-layer chromatography
- 5.1. Introduction
- 5.2. Sample applicators
- 5.3. Developing chambers
- 5.4. Derivatization
- 5.5. Densitometers for evaluation
- Chapter 6. Theory and instrumentation for in situ detection
- 6.1. Introduction
- 6.2. Theory for in situ densitometric detection
- 6.3. Instrumentation for in situ densitometric detection
- 6.4. In situ mass spectrometry
- 6.5. In situ radioisotope detection
- Chapter 7. Advanced spectroscopic detectors for identification and quantification: infrared, Raman, and nuclear magnetic resonance
- 7.1. Introduction
- 7.2. Hyphenation of thin-layer chromatography with infrared spectroscopy
- 7.3. Offline methods
- 7.4. In situ infrared measurements
- 7.5. Raman spectroscopy
- 7.6. Thin-layer chromatography–surface-enhanced Raman spectroscopy
- 7.7. Dynamic surface-enhanced Raman spectroscopy
- 7.8. TLC-NMR spectroscopy
- Chapter 8. Advanced spectroscopic detectors for identification and quantification: mass spectrometry
- 8.1. Introduction
- 8.2. Classification of TLC-MS techniques
- 8.3. Indirect sampling TLC-MS
- 8.4. Direct sampling TLC-MS
- 8.5. High-throughput TLC-MS devices and quantification analysis
- 8.6. Conclusion
- Chapter 9. Staining and derivatization techniques for visualization in planar chromatography
- 9.1. Problem overview
- 9.2. Reagent application, equipment, and protocols
- 9.3. Techniques for heating or drying layers after development
- 9.4. Common detection protocols for target compounds
- 9.5. Simple devices for planar chromatograms digitalization
- 9.6. Conclusions
- Chapter 10. Effects-directed detection: cell-based assays
- 10.1. Introduction
- 10.2. Thin-layer chromatography—antimicrobial detection
- 10.3. (HP)TLC-hormonal, pathogenicity, and genotoxicity bioassays
- 10.4. Applications
- 10.5. Conclusions
- Chapter 11. Effect-directed detection: chemical and enzyme-based assays
- 11.1. Introduction
- 11.2. Enzyme inhibitors
- 11.3. Antioxidant activity
- Chapter 12. Validation of thin-layer chromatographic methods
- 12.1. Introduction
- 12.2. Classical and combined methodologies: common validation characteristics
- 12.3. Classical method validation: performance characteristics and their possible improvements
- 12.4. Combined method validation
- 12.5. Examples of combined validation design application to TLC/HPTLC methods
- 12.6. Conclusion
- Chapter 13. Data analysis tools in thin-layer chromatography
- 13.1. Introduction
- 13.2. Software for evaluation of thin-layer chromatograms
- 13.3. Signal preprocessing—connection between the image of a thin-layer chromatogram and chemometric evaluation
- 13.4. Chemometric techniques for evaluation of TLC data
- 13.5. HPTLC image–based quantitative analysis
- Chapter 14. Microfluidic and small-scale planar separation systems
- 14.1. Important trends influencing separation science
- 14.2. Microfluidic planar separation systems
- 14.3. Small-scale planar separation systems
- 14.4. Outlook and perspectives
- Section 2. Applications of thin-layer chromatography
- Chapter 15. Application of thin-layer chromatography to the analysis of saccharides
- 15.1. Introduction
- 15.2. Visualization reagents
- 15.3. Separation of mono- and disaccharides
- 15.4. Separation of oligosaccharides
- 15.5. Saccharide mapping
- Chapter 16. Applications of thin-layer chromatography to the analysis of lipids
- 16.1. Introduction
- 16.2. Thin-layer chromatographic lipid analysis
- 16.3. Applications of TLC for lipid separations
- 16.4. Coupling TLC with mass spectrometric detection
- 16.5. Summary and outlook
- Chapter 17. Applications in food analysis
- 17.1. Complexity of food samples
- 17.2. Conventional applications
- 17.3. Simplicity and sustainability
- 17.4. Benefits of multidetection
- 17.5. Method performance
- 17.6. Powerful hyphenations and multiplex assays
- 17.7. Portable food analysis system
- 17.8. Conclusions
- Chapter 18. Applications of thin-layer chromatography in environmental analysis
- 18.1. Introduction
- 18.2. Target compound methods
- 18.3. Effect-directed methods
- 18.4. Planar solid-phase extraction
- Chapter 19. Applications of thin-layer chromatography in the pharmaceutical industry
- 19.1. Introduction
- 19.2. Cleaning validation of pharmaceutical equipment
- 19.3. Enantioseparation, determination and purity evaluation of chiral drugs
- 19.4. Analysis of mono-component, two-component and multicomponent pharmaceuticals
- 19.5. Biopharmaceutical and preformulation analysis of pharmaceuticals
- Chapter 20. Applications of thin-layer chromatography in the quality control of botanicals
- 20.1. Introduction
- 20.2. Sample preparation
- 20.3. Thin-layer chromatography in authentication of botanicals
- 20.4. Low-temperature thin-layer chromatography as a new old option
- 20.5. Effect-directed detection in quality control of botanicals
- 20.6. Thin-layer chromatography for selection of underquality botanicals
- 20.7. Conclusion
- Chapter 21. Applications of thin-layer chromatography to the quality control of dietary supplements
- 21.1. Introduction
- 21.2. Analytical challenges
- 21.3. Instrumental TLC
- 21.4. Analysis of bioactive ingredients
- 21.5. Fingerprinting and detection of adulterants
- Index
- Edition: 2
- Published: January 1, 2023
- Imprint: Elsevier
- No. of pages: 690
- Language: English
- Paperback ISBN: 9780323999700
- eBook ISBN: 9780323983013
CP
Colin Poole
Professor Colin Poole is internationally known in the field of thin-layer chromatography and is an editor of the Journal of Chromatography and former editor of the Journal of Planar Chromatography – Modern TLC. He has authored several books on chromatography, recent examples being The Essence of Chromatography published by Elsevier (2003), and Gas Chromatography published by Elsevier (2012). He is the author of approximately 400 research articles, many of which deal with thin-layer chromatography, and is co-chair of the biennial “International Symposium on High-Performance Thin-Layer Chromatography”.
Affiliations and expertise
Department of Chemistry, Wayne State University, Detroit, MI, USARead Instrumental Thin-Layer Chromatography on ScienceDirect