Instrumental Thin-Layer Chromatography

1st Edition - September 22, 2014

Author: Colin Poole

Hardback ISBN:

9 7 8 - 0 - 1 2 - 4 1 7 2 2 3 - 4

eBook ISBN:

9 7 8 - 0 - 1 2 - 4 1 7 2 8 4 - 5

Instrumental Thin-Layer Chromatography delivers comprehensive coverage of this separation tool with particular emphasis on how this tool can be used in advanced laboratories and… Read more

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Instrumental Thin-Layer Chromatography delivers comprehensive coverage of this separation tool with particular emphasis on how this tool can be used in advanced laboratories and integrated into problem-solving scenarios. Significant improvements in instrumentation have outpaced the development of information resources that describe the latest state-of-the-art and demonstrate the full capabilities of TLC. This book provides a contemporary picture of the fundamentals and practical applications of TLC at a level suitable for the needs of professional scientists with interests in project management where TLC is a common tool. Compact, highly focused chapters convey essential information that defines modern TLC and how it can be effectively implemented in most areas of laboratory science. Numerous figures and tables provide access to material not normally found in a single source yet are required by working scientists.

Chapter 1. Milestones, Core Concepts, and Contrasts
- 1.1. Introduction
- 1.2. Milestones
- 1.3. Attributes of a Planar Format
- 1.4. Consequences of Capillary-Controlled Flow
- 1.5. Solvent-Strength Gradients
- 1.6. Multidimensional Separations
- 1.7. Conclusions
Chapter 2. High-Performance Precoated Stationary Phases
- 2.1. Introduction
- 2.2. Inorganic Oxide Sorbents
- 2.3. Chemically Bonded Sorbents
- 2.4. Cellulose
- 2.5. Chiral Sorbents
- 2.6. Conclusions
Chapter 3. Ultrathin and Nanostructured Stationary Phases
- 3.1. Introduction
- 3.2. Monolithic Silica Gels
- 3.3. Monolithic Polymers
- 3.4. Electrospun Nanofibers
- 3.5. Nanostructured Thin Films
- 3.6. Carbon Nanotube-Templated Layers
- 3.7. Advanced Instrumentation and Techniques
- 3.8. Conclusions
Chapter 4. Automated Multiple Development
- 4.1. Introduction
- 4.2. Applications
- 4.3. Future Trends
- 4.4. Conclusions
Chapter 5. Forced-Flow Development in Overpressured Layer Chromatography
- 5.1. Introduction
- 5.2. Analytical and Preparative OPLC Processes
- 5.3. Instruments and Layers for OPLC
- 5.4. Main Characteristics of OPLC
- 5.5. Analytical and Preparative Applications
- 5.6. Conclusion
Chapter 6. Pressurized Planar Electrochromatography
- 6.1. Introduction
- 6.2. Theoretical Background
- 6.3. Development of Equipment for PPEC
- 6.4. Advantages of PPEC
- 6.5. Applications
- 6.6. Challenges and Conclusions
Chapter 7. Theory and Instrumentation for In situ Detection
- 7.1. Introduction
- 7.2. Theory for In situ Densitometric Detection
- 7.3. Instrumentation for In situ Densitometric Detection
- 7.4. In situ Mass-Spectrometry
- 7.5. In situ Radioisotope Detection
Chapter 8. Staining and Derivatization Techniques for Visualization in Planar Chromatography
- 8.1. Problem Overview
- 8.2. Reagent Application, Equipment, and Protocols
- 8.3. Techniques for Heating or Drying Layers after Development
- 8.4. Common Detection Protocols for Target Compounds
- 8.5. Conclusions
Chapter 9. Advanced Spectroscopic Detectors for Identification and Quantification: UV—Visible, Fluorescence, and Infrared Spectroscopy
- 9.1. Introduction
- 9.2. Advanced Spectroscopic Detectors for Identification and Quantification
- 9.3. Conclusion
Chapter 10. Advanced Spectroscopic Detectors for Identification and Quantification: Mass Spectrometry
- 10.1. Introduction
- 10.2. Classification of TLC-MS Techniques
- 10.3. Indirect Sampling TLC-MS
- 10.4. Direct Sampling TLC-MS
- 10.5. High-Throughput TLC-MS Devices and Quantification Analysis
- 10.6. Conclusion
Chapter 11. Effects-Directed Biological Detection: Bioautography
- 11.1. Introduction
- 11.2. Applications of Bioautographic Tests
- 11.3. Perspectives
Chapter 12. Solvent Selection and Method Development
- 12.1. Introduction
- 12.2. Problem Definition
- 12.3. Mode Selection
- 12.4. Mobile Phase Selection
- 12.5. Conclusions
Chapter 13. Validation of Thin Layer Chromatographic Methods
- 13.1. Introduction
- 13.2. Method Validation Using the Classic Approach
- 13.3. Alternative Method Validation Approach Using Accuracy Profiles
- 13.4. Conclusion
Chapter 14. Separation of (Phospho)Lipids by Thin-Layer Chromatography
- 14.1. Introduction
- 14.2. Separation of Lipids by TLC
- 14.3. Applications
- 14.4. MALDI for MS Detection
- 14.5. Summary and Outlook
Chapter 15. Applications in Food Analysis
- 15.1. Introduction
- 15.2. HPTLC in Food Analysis
- 15.3. Sample Preparation, Hyphenation, and New Possibilities
- 15.4. Suitability and Capabilities
- 15.5. Conclusion
Chapter 16. Environmental Applications
- 16.1. Introduction
- 16.2. HPTLC in Environmental Analysis
- 16.3. Conclusion
Chapter 17. Pharmaceutical Applications of High Performance Thin Layer Chromatography
- 17.1. Introduction
- 17.2. Quantitative Analysis
- 17.3. Pharmaceutical Applications
- 17.4. Conclusions
Chapter 18. Utility of Thin-Layer Chromatography in the Assessment of the Quality of Botanicals
- 18.1. Introduction
- 18.2. Method Validation
- 18.3. Hyphenated Techniques and Chemometrics in Herbal Analysis
- 18.4. Applications of TLC in the Field of Herbal Products/Botanicals
- 18.5. Use of HPTLC by the American Herbal Pharmacopoeia
- 18.6. Recent Advances in HPTLC-based Quality Control of Botanicals and Dietary Supplements
- 18.7. Conclusions
Chapter 19. Analysis of Plant Material
- 19.1. Introduction
- 19.2. Single Development
- 19.3. Special Development Techniques
- 19.4. Coupling Planar Chromatography with Column Chromatography
- 19.5. Forced-Flow Development
- 19.6. Chemical Fingerprinting of Plant Materials
- 19.7. Effect-Directed Analysis of Plant Materials
- 19.8. Image Processing
Chapter 20. Analysis of Dyes and Inks
- 20.1. Introduction
- 20.2. Dyes
- 20.3. Inks
- 20.4. Conclusion
Chapter 21. Analysis of Dietary Supplements
- 21.1. Introduction
- 21.2. Analytical Challenges
- 21.3. Instrumental TLC
- 21.4. Analysis of Bioactive Ingredients
- 21.5. Fingerprinting and Detection of Adulterants
- 21.6. Conclusions
Index