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Instrumental Thin-Layer Chromatography
1st Edition - September 22, 2014
Author: Colin Poole
Hardback ISBN:9780124172234
9 7 8 - 0 - 1 2 - 4 1 7 2 2 3 - 4
eBook ISBN:9780124172845
9 7 8 - 0 - 1 2 - 4 1 7 2 8 4 - 5
Instrumental Thin-Layer Chromatography delivers comprehensive coverage of this separation tool with particular emphasis on how this tool can be used in advanced laboratories and… Read more
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Instrumental Thin-Layer Chromatography delivers comprehensive coverage of this separation tool with particular emphasis on how this tool can be used in advanced laboratories and integrated into problem-solving scenarios. Significant improvements in instrumentation have outpaced the development of information resources that describe the latest state-of-the-art and demonstrate the full capabilities of TLC. This book provides a contemporary picture of the fundamentals and practical applications of TLC at a level suitable for the needs of professional scientists with interests in project management where TLC is a common tool. Compact, highly focused chapters convey essential information that defines modern TLC and how it can be effectively implemented in most areas of laboratory science. Numerous figures and tables provide access to material not normally found in a single source yet are required by working scientists.
Contributions written by recognized authoritative and visionary experts
Focuses on state-of-the-art instrumental thin-layer chromatography and advanced applications across many areas
Provides guidance on the analysis of complex, dirty mixtures of compounds
Offers a cost-effective analytic technique for laboratories working under strict budgets
Analytical chemists, scientists, and researchers working in drug discovery, food and plant science, environmental science, and forensics; graduate students in these disciplines
Chapter 1. Milestones, Core Concepts, and Contrasts
Chapter 12. Solvent Selection and Method Development
12.1. Introduction
12.2. Problem Definition
12.3. Mode Selection
12.4. Mobile Phase Selection
12.5. Conclusions
Chapter 13. Validation of Thin Layer Chromatographic Methods
13.1. Introduction
13.2. Method Validation Using the Classic Approach
13.3. Alternative Method Validation Approach Using Accuracy Profiles
13.4. Conclusion
Chapter 14. Separation of (Phospho)Lipids by Thin-Layer Chromatography
14.1. Introduction
14.2. Separation of Lipids by TLC
14.3. Applications
14.4. MALDI for MS Detection
14.5. Summary and Outlook
Chapter 15. Applications in Food Analysis
15.1. Introduction
15.2. HPTLC in Food Analysis
15.3. Sample Preparation, Hyphenation, and New Possibilities
15.4. Suitability and Capabilities
15.5. Conclusion
Chapter 16. Environmental Applications
16.1. Introduction
16.2. HPTLC in Environmental Analysis
16.3. Conclusion
Chapter 17. Pharmaceutical Applications of High Performance Thin Layer Chromatography
17.1. Introduction
17.2. Quantitative Analysis
17.3. Pharmaceutical Applications
17.4. Conclusions
Chapter 18. Utility of Thin-Layer Chromatography in the Assessment of the Quality of Botanicals
18.1. Introduction
18.2. Method Validation
18.3. Hyphenated Techniques and Chemometrics in Herbal Analysis
18.4. Applications of TLC in the Field of Herbal Products/Botanicals
18.5. Use of HPTLC by the American Herbal Pharmacopoeia
18.6. Recent Advances in HPTLC-based Quality Control of Botanicals and Dietary Supplements
18.7. Conclusions
Chapter 19. Analysis of Plant Material
19.1. Introduction
19.2. Single Development
19.3. Special Development Techniques
19.4. Coupling Planar Chromatography with Column Chromatography
19.5. Forced-Flow Development
19.6. Chemical Fingerprinting of Plant Materials
19.7. Effect-Directed Analysis of Plant Materials
19.8. Image Processing
Chapter 20. Analysis of Dyes and Inks
20.1. Introduction
20.2. Dyes
20.3. Inks
20.4. Conclusion
Chapter 21. Analysis of Dietary Supplements
21.1. Introduction
21.2. Analytical Challenges
21.3. Instrumental TLC
21.4. Analysis of Bioactive Ingredients
21.5. Fingerprinting and Detection of Adulterants
21.6. Conclusions
Index
No. of pages: 652
Language: English
Published: September 22, 2014
Imprint: Elsevier
Hardback ISBN: 9780124172234
eBook ISBN: 9780124172845
CP
Colin Poole
Professor Colin Poole is internationally known in the field of thin-layer chromatography and is an editor of the Journal of Chromatography and former editor of the Journal of Planar Chromatography – Modern TLC. He has authored several books on chromatography, recent examples being The Essence of Chromatography published by Elsevier (2003), and Gas Chromatography published by Elsevier (2012). He is the author of approximately 400 research articles, many of which deal with thin-layer chromatography, and is co-chair of the biennial “International Symposium on High-Performance Thin-Layer Chromatography”.
Affiliations and expertise
Department of Chemistry, Wayne State University, Detroit, MI, USA