Instrumental Thin-Layer Chromatography
- 1st Edition - September 24, 2014
- Author: Colin Poole
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 4 1 7 2 2 3 - 4
- eBook ISBN:9 7 8 - 0 - 1 2 - 4 1 7 2 8 4 - 5
Instrumental Thin-Layer Chromatography delivers comprehensive coverage of this separation tool with particular emphasis on how this tool can be used in advanced laboratories and in… Read more
Instrumental Thin-Layer Chromatography delivers comprehensive coverage of this separation tool with particular emphasis on how this tool can be used in advanced laboratories and integrated into problem-solving scenarios. Significant improvements in instrumentation have outpaced the development of information resources that describe the latest state-of-the-art and demonstrate the full capabilities of TLC. This book provides a contemporary picture of the fundamentals and practical applications of TLC at a level suitable for the needs of professional scientists with interests in project management where TLC is a common tool. Compact, highly focused chapters convey essential information that defines modern TLC and how it can be effectively implemented in most areas of laboratory science. Numerous figures and tables provide access to material not normally found in a single source yet are required by working scientists.
- Contributions written by recognized authoritative and visionary experts
- Focuses on state-of-the-art instrumental thin-layer chromatography and advanced applications across many areas
- Provides guidance on the analysis of complex, dirty mixtures of compounds
- Offers a cost-effective analytic technique for laboratories working under strict budgets
Analytical chemists, scientists, and researchers working in drug discovery, food and plant science, environmental science, and forensics; graduate students in these disciplines
- Chapter 1. Milestones, Core Concepts, and Contrasts
- 1.1. Introduction
- 1.2. Milestones
- 1.3. Attributes of a Planar Format
- 1.4. Consequences of Capillary-Controlled Flow
- 1.5. Solvent-Strength Gradients
- 1.6. Multidimensional Separations
- 1.7. Conclusions
- Chapter 2. High-Performance Precoated Stationary Phases
- 2.1. Introduction
- 2.2. Inorganic Oxide Sorbents
- 2.3. Chemically Bonded Sorbents
- 2.4. Cellulose
- 2.5. Chiral Sorbents
- 2.6. Conclusions
- Chapter 3. Ultrathin and Nanostructured Stationary Phases
- 3.1. Introduction
- 3.2. Monolithic Silica Gels
- 3.3. Monolithic Polymers
- 3.4. Electrospun Nanofibers
- 3.5. Nanostructured Thin Films
- 3.6. Carbon Nanotube-Templated Layers
- 3.7. Advanced Instrumentation and Techniques
- 3.8. Conclusions
- Chapter 4. Automated Multiple Development
- 4.1. Introduction
- 4.2. Applications
- 4.3. Future Trends
- 4.4. Conclusions
- Chapter 5. Forced-Flow Development in Overpressured Layer Chromatography
- 5.1. Introduction
- 5.2. Analytical and Preparative OPLC Processes
- 5.3. Instruments and Layers for OPLC
- 5.4. Main Characteristics of OPLC
- 5.5. Analytical and Preparative Applications
- 5.6. Conclusion
- Chapter 6. Pressurized Planar Electrochromatography
- 6.1. Introduction
- 6.2. Theoretical Background
- 6.3. Development of Equipment for PPEC
- 6.4. Advantages of PPEC
- 6.5. Applications
- 6.6. Challenges and Conclusions
- Chapter 7. Theory and Instrumentation for In situ Detection
- 7.1. Introduction
- 7.2. Theory for In situ Densitometric Detection
- 7.3. Instrumentation for In situ Densitometric Detection
- 7.4. In situ Mass-Spectrometry
- 7.5. In situ Radioisotope Detection
- Chapter 8. Staining and Derivatization Techniques for Visualization in Planar Chromatography
- 8.1. Problem Overview
- 8.2. Reagent Application, Equipment, and Protocols
- 8.3. Techniques for Heating or Drying Layers after Development
- 8.4. Common Detection Protocols for Target Compounds
- 8.5. Conclusions
- Chapter 9. Advanced Spectroscopic Detectors for Identification and Quantification: UV—Visible, Fluorescence, and Infrared Spectroscopy
- 9.1. Introduction
- 9.2. Advanced Spectroscopic Detectors for Identification and Quantification
- 9.3. Conclusion
- Chapter 10. Advanced Spectroscopic Detectors for Identification and Quantification: Mass Spectrometry
- 10.1. Introduction
- 10.2. Classification of TLC-MS Techniques
- 10.3. Indirect Sampling TLC-MS
- 10.4. Direct Sampling TLC-MS
- 10.5. High-Throughput TLC-MS Devices and Quantification Analysis
- 10.6. Conclusion
- Chapter 11. Effects-Directed Biological Detection: Bioautography
- 11.1. Introduction
- 11.2. Applications of Bioautographic Tests
- 11.3. Perspectives
- Chapter 12. Solvent Selection and Method Development
- 12.1. Introduction
- 12.2. Problem Definition
- 12.3. Mode Selection
- 12.4. Mobile Phase Selection
- 12.5. Conclusions
- Chapter 13. Validation of Thin Layer Chromatographic Methods
- 13.1. Introduction
- 13.2. Method Validation Using the Classic Approach
- 13.3. Alternative Method Validation Approach Using Accuracy Profiles
- 13.4. Conclusion
- Chapter 14. Separation of (Phospho)Lipids by Thin-Layer Chromatography
- 14.1. Introduction
- 14.2. Separation of Lipids by TLC
- 14.3. Applications
- 14.4. MALDI for MS Detection
- 14.5. Summary and Outlook
- Chapter 15. Applications in Food Analysis
- 15.1. Introduction
- 15.2. HPTLC in Food Analysis
- 15.3. Sample Preparation, Hyphenation, and New Possibilities
- 15.4. Suitability and Capabilities
- 15.5. Conclusion
- Chapter 16. Environmental Applications
- 16.1. Introduction
- 16.2. HPTLC in Environmental Analysis
- 16.3. Conclusion
- Chapter 17. Pharmaceutical Applications of High Performance Thin Layer Chromatography
- 17.1. Introduction
- 17.2. Quantitative Analysis
- 17.3. Pharmaceutical Applications
- 17.4. Conclusions
- Chapter 18. Utility of Thin-Layer Chromatography in the Assessment of the Quality of Botanicals
- 18.1. Introduction
- 18.2. Method Validation
- 18.3. Hyphenated Techniques and Chemometrics in Herbal Analysis
- 18.4. Applications of TLC in the Field of Herbal Products/Botanicals
- 18.5. Use of HPTLC by the American Herbal Pharmacopoeia
- 18.6. Recent Advances in HPTLC-based Quality Control of Botanicals and Dietary Supplements
- 18.7. Conclusions
- Chapter 19. Analysis of Plant Material
- 19.1. Introduction
- 19.2. Single Development
- 19.3. Special Development Techniques
- 19.4. Coupling Planar Chromatography with Column Chromatography
- 19.5. Forced-Flow Development
- 19.6. Chemical Fingerprinting of Plant Materials
- 19.7. Effect-Directed Analysis of Plant Materials
- 19.8. Image Processing
- Chapter 20. Analysis of Dyes and Inks
- 20.1. Introduction
- 20.2. Dyes
- 20.3. Inks
- 20.4. Conclusion
- Chapter 21. Analysis of Dietary Supplements
- 21.1. Introduction
- 21.2. Analytical Challenges
- 21.3. Instrumental TLC
- 21.4. Analysis of Bioactive Ingredients
- 21.5. Fingerprinting and Detection of Adulterants
- 21.6. Conclusions
- Index
- Edition: 1
- Published: September 24, 2014
- Language: English
CP
Colin Poole
Professor Colin Poole is internationally known in the field of thin-layer chromatography and is an editor of the Journal of Chromatography and former editor of the Journal of Planar Chromatography – Modern TLC. He has authored several books on chromatography, recent examples being The Essence of Chromatography published by Elsevier (2003), and Gas Chromatography published by Elsevier (2012). He is the author of approximately 400 research articles, many of which deal with thin-layer chromatography, and is co-chair of the biennial “International Symposium on High-Performance Thin-Layer Chromatography”.
Affiliations and expertise
Department of Chemistry, Wayne State University, Detroit, MI, USARead Instrumental Thin-Layer Chromatography on ScienceDirect