Immuno-oncology and immunotherapy Part B
- 1st Edition, Volume 190 - November 7, 2024
- Imprint: Academic Press
- Editors: Lorenzo Galluzzi, Norma Bloy, Maud Charpentier
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 2 9 6 1 6 - 1
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 2 9 6 1 7 - 8
Immuno-Oncology And Immunotherapy – Part B Volume 190, in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leade… Read more
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Request a sales quoteImmuno-Oncology And Immunotherapy – Part B Volume 190, in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. In this update, chapters include Machine learning methods to assess metastatic burden, Development of pre-clinical models, Tumor microenvironment analysis by multiparametric flow cytometry or/and mass cytometry, Measure of therapy induced cell senescence, and much more.
- Authored by established and active cell biologists and immunologist and drawn from international sources.
- Includes in-depth coverage and detailed protocols.
- Present a highly specialized group of topics that delve deep into new updates and future prospects.
This volume is addressed to experts in the field who may want to expand their technical horizons and to newcomers who need detailed introductions to basic techniques.
- Immuno-Oncology and Immunotherapy - Part B
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Chapter One Flow cytometry analysis of myeloid derived suppressor cells using 6 color labeling
- Abstract
- Keywords
- 1 Introduction
- 2 Materials
- 2.1 Common consumables
- 2.2 Samples and reagents
- 2.3 Equipment
- 2.4 Software
- 3 Methods
- 3.1 Blood sample preparation
- 3.2 Flow cytometry acquisition
- 3.3 Data analysis
- 4 Concluding remarks
- References
- Chapter Two In vitro screening methods of novel immune checkpoint inhibitors related to T cell infiltration and anti-PD-1 resistance
- Abstract
- Keyword
- 1 Introduction
- 2 Materials
- 2.1 Common disposables
- 2.2 Cells and reagents
- 2.3 Equipments
- 2.4 Software
- 3 Methods
- 3.1 Cell culture
- 3.2 Primary lymphocytes extraction and CFSE labeling
- 3.3 In vitro CD8+ T cell migration assay
- 3.4 Immune cells infiltration assay in tumor sphere
- 4 Data analysis
- 4.1 In vitro CD8+ T cell migration assay
- 4.2 Immune cells infiltration assay in tumor sphere
- 5 Notes
- 6 Concluding remarks
- Conflict of interest
- References
- Chapter Three Machine learning approach to assess brain metastatic burden in preclinical models
- Abstract
- Keywords
- 1 Introduction
- 2 Materials
- 2.1 Common disposables
- 2.2 Reagents
- 2.3 Equipment
- 2.4 Software
- 3 Methods
- 3.1 Material preparation
- 3.2 Sample collection
- 3.3 Imaging
- 3.4 Machine learning algorithm development
- 3.5 Application of the algorithm
- 4 Notes
- 5 Concluding remarks
- Acknowledgments
- References
- Chapter Four Cytofluorometric analysis of the maturation and activation of bone marrow-derived dendritic cells to assess immunogenic cell death
- Abstract
- Keywords
- 1 Introduction
- 2 Materials
- 2.1 Cells
- 2.2 Materials and reagents
- 2.3 Equipment
- 2.4 Software
- 3 Protocol
- 3.1 Isolation and culture of murine BMDCs
- 3.2 Culture of MCA205 fibrosarcoma cells
- 3.3 MCA205 ICD induction
- 3.4 Analysis of the maturation and activation status of DCs in the presence of MTX-induced cancer cells
- 3.5 Flow cytometry data acquisition
- 3.6 Gating strategy and data analysis
- 4 Notes
- 5 Concluding Remarks
- Competing interests
- References
- Chapter Five Functional evaluation of circulating anti-cancer antibodies with a 3D tumor cell growth inhibition assay
- Abstract
- Keywords
- 1 Introduction
- 2 Materials
- 2.1 Common disposables
- 2.2 Cells and reagents
- 2.3 Equipment
- 2.4 Software
- 3 Methods
- 3.1 Combinations/treatments to test
- 3.2 Assay procedures
- 4 Notes
- 5 Concluding remarks
- Conflicts of interest
- References
- Chapter Six Spatial immunophenotyping of FFPE tissues by imaging mass cytometry
- Abstract
- Keywords
- 1 Introduction
- 2 Materials
- 2.1 Common disposables
- 2.2 Reagents and tissues
- 2.3 Equipment
- 2.4 Software
- 3 Methods
- 3.1 Antigen retrieval
- 3.2 Antibody preparation and staining
- 3.3 DNA staining
- 3.4 Imaging
- 3.5 Image analysis
- 4 Notes
- 5 Concluding remarks
- Acknowledgments
- Conflicts of interest
- References
- Further-reading
- Chapter Seven Processing human colon cancer specimens for in vitro cytotoxicity assays
- Abstract
- Keywords
- 1 Introduction
- 2 Materials
- 2.1 Common disposables
- 2.2 Reagents
- 2.3 Preparation of solutions
- 2.4 Equipment
- 2.5 Software
- 3 Methods
- 3.1 Tumor collection and processing for cryopreservation
- 3.2 Tumor thawing and digestion
- 3.3 Depletion of CD45+ cells (optional)
- 3.4 Cytotoxicity assay preparation
- 3.5 Cell staining and analysis
- 4 Notes
- 5 Concluding remarks
- Conflict of interest
- References
- Chapter Eight An orthotopic metastatic xenograft model of colorectal cancer
- Abstract
- Keywords
- 1 Introduction
- 2 Materials
- 2.1 Common disposables
- 2.2 Cells and reagents
- 2.3 Preparation of solutions
- 2.4 Mouse strains
- 2.5 Equipment
- 2.6 Software
- 3 Methods
- 3.1 Generation of luciferase/GFP+ cell lines
- 3.2 Cell culture and treatments
- 3.3 Intracaecal tumor implantation (surgery)
- 3.4 In vivo tumor growth monitoring
- 3.5 Euthanasia and sample collection for histopathology assessment
- 4 Notes
- 5 Concluding remarks
- Conflict of interest
- References
- Chapter Nine A preclinical mouse model of hepatic metastasis to instruct effective treatment modalities
- Abstract
- Keywords
- 1 Introduction
- 2 Rationale
- 3 Preclinical mouse model of hepatic metastasis
- 3.1 Materials, reagents and equipment
- 3.2 Protocol
- 3.3 Identify and monitor metastatic dissemination in the liver
- 3.4 Example of model exploitation
- 4 Concluding remarks
- Acknowledgments
- References
- Chapter Ten Myeloid-derived suppressor cells: Identification and function
- Abstract
- Keywords
- 1 Introduction
- 1.1 PMN-MDSC and immune cell interactions
- 1.2 PMN-MDSC in tumors
- 1.3 Identification of PMN-MDSC
- 1.4 Materials
- 2 Isolation of MDSC
- 3 Purification of PMN-MDSC
- 4 Flow cytometric phenotype of PMN-MDSC
- 5 Antibodies for human PMN-MDSC identification
- 6 Functional evaluation of PMN-MDSC
- 7 Concluding remarks
- Conflict of interest
- References
- Chapter Eleven Measuring the impact of therapy-induced senescence on NK cell phenotypes in cancer
- Abstract
- Keywords
- 1 Introduction
- 2 Materials
- 2.1 Common disposables
- 2.2 Mice, cell lines, and reagents
- 2.3 Equipment
- 2.4 Software
- 3 Methods
- 3.1 Generation of pancreatic cancer mouse model (see Notes 3, 4, and 10)
- 3.2 Senescence-inducing treatment regimens (see Note 22)
- 3.3 Flow cytometry analysis of live tumor tissue (see Note 26)
- 3.4 Flow cytometry analysis after ex vivo stimulation (see Note 33)
- 3.5 NK cell migration assays
- 3.6 NK cell cytotoxicity assays (see Note 40)
- 4 Notes
- 5 Concluding remarks
- Conflicts of interest
- References
- Chapter Twelve Selective expansion of anti-tumor innate lymphocytes in long-term cultures after a single BCG pulse
- Abstract
- Keywords
- 1 Introduction
- 2 Materials
- 3 Methods
- 3.1 PBMC co-culture with BCG stimulated with minimal-dose cytokines after 7 days in culture
- 3.2 Phenotype characterization
- 3.3 Functional assays
- 4 Conclusion
- 5 Notes
- References
- Edition: 1
- Volume: 190
- Published: November 7, 2024
- Imprint: Academic Press
- No. of pages: 318
- Language: English
- Hardback ISBN: 9780443296161
- eBook ISBN: 9780443296178
LG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USANB
Norma Bloy
Norma received her master’s degree in health biology from Paris Saclay. In 2013, she joined the laboratory of Dr. Guido Kroemer at the Centre de Recherche des Cordeliers (Paris, France) and at Gustave Roussy (Villejuif, France), the largest center for oncological patients in Europe. She worked on several projects linked to immunosurveillance, culminating with her obtaining her PhD in 2017 with an original work on “Immunogenic stress and death of cancer cells: Contribution of antigenicity vs adjuvanticity to immunosurveillance”. She then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Galluzzi. Her current research is focused on investigating resistance to immunotherapy in a mouse model of HR+ breast cancer.
Affiliations and expertise
Weill Cornell Medical College, USAMC
Maud Charpentier
Maud Charpentier received her M.Sc. in Cellular Biology and her Ph.D. in Immunology from the University of Nantes, France. She chose to pursue an academic career and continued her postdoctoral training in the United States. She joined the Department of Radiation Oncology at Weill Cornell Medicine under the mentorship of Dr. Sandra Demaria. Maud has a long-standing interest in the anti-tumor immune response and its role in controlling cancer progression and treatment outcomes. Her research focuses on understanding the synergy between radiation therapy and immunotherapy in solid tumors, with the aim of overcoming resistance to treatment and developing innovative therapeutic approaches in preclinical models.
Affiliations and expertise
Weill Cornell Medicine, USARead Immuno-oncology and immunotherapy Part B on ScienceDirect